Learn More Modifying Enzymes–Thermo Scientific
Alkaline phosphatase and polynucleotide kinase for DNA and RNA dephosphorylation or phosphorylation., Properties of RNA polymerases for in vitro transcription, RNA labeling, synthesis of antisense RNA and siRNA, studies of RNA splicing and secondary structure.
We offer an extensive portfolio of Thermo Scientific restriction enzymes and modifying enzymes including DNA and RNA polymerases, phosphatases, kinases, and nucleases. Whatever your downstream applications are, it is critical to use reliable, high-quality, high-purity reagents in your experiments.
Learn More Modifying Enzyme Buffers
Thermo Scientific DNA/RNA modifying enzymes are supplied with optimized reaction buffers. However, it is often convenient to use these enzymes in other buffers for experiments that involve multiple enzymatic reactions.
Learn More Anza Restriction Enzymes
Introducing the Invitrogen Anza Restriction Enzyme Cloning System, a complete, one-buffer system of restriction enzymes and DNA-modifying enzymes–for beautifully simple cloning. Request a free Anza poster Download brochure One buffer for all restriction and DNA modifying enzymes One digestion...
Learn More Restriction Enzyme Digestion and Ligation
Cloning by restriction enzyme digestion and ligation is a simple and easy way of moving a fragment of double-stranded DNA from one plasmid to another. Choosing restriction enzymes whose recognition sequences flank your gene of interest Incubating the reaction for the recommended amount of time...
Thermo Fisher Scientific is a global leader in the manufacture and supply of nucleic acid modifying enzymes and polymerases. We offer a combination of proprietary as well as generic off-the-shelf enzymes, and can also custom-manufacture on your behalf.
Learn More 176 Restriction Enzymes, 1 Buffer
It's cloning time—you want to excise your insert from a vector with two different enzymes. Looking at the old enzyme poster on the fridge you notice that they don't work in the same buffer. Great! Add an extra hour to your schedule for back to back digestions.
Learn More Molecular Biology
Our Thermo Scientific molecular biology products are designed to deliver reproducibility and performance in everyday applications including reverse transcription, PCR, nucleic acid electrophoresis, restriction enzyme digests, and cloning.
Learn More CloningBench Cloning Tools App
CloningBench is designed to provide you with useful and essential tools to help guide your cloning experiments at the bench or on the move. With over 24,000 downloads to-date, join in by downloading and supercharge your cloning success at the bench.
Learn More TOPO Resources
Learn more about Invitrogen TOPO cloning technology, directional TOPO and download TOPO vector maps., pCR 2.1-TOPO (TOPO TA for subcloning) pCR 4-TOPO (TOPO TA for sequencing) in vitro, pCR-XL-2-TOPO (TOPO long fragment cloning) pCR Blunt II-TOPO (TOPO Blunt-end for subcloning) pCR 4 Blunt-TOPO...
Learn More TOPO-TA Cloning
Clone PCR-amplified DNA fragments (blunt or A-overhang) directly into a choice of over 40 subcloning, sequencing, or expression vectors in just 5 minutes—and obtain up to 95% recombinant clones. Quick— Efficient— Troubleshooting tool—, Figure 1. The TOPO cloning protocol.
Learn More Molecular Biology Resource Library
Welcome to the Thermo Scientific resource library. This page contains valuable links and supporting resources for standard molecular biology techniques, as well as specialty applications. It also provides easy access to a large variety of Thermo Scientific webinars, videos, articles, and more.
Learn More Life Science Tools for Commercial Supply
Our specialized products, innovative technologies and customized workflow solutions help expand and compliment the portfolios of life science tool providers., PCR & RT-PCR enzymes DNA/RNA modifying enzymes Restriction enzymes Molecular cloning enzymes Lyo-ready enzymes Enzyme engineering and...
Learn More In Vitro Transcription & Translation
In vitro RNA transcription reactions are generally used for two distinct purposes: the synthesis of labeled probes, and the synthesis of large amounts of unlabeled RNA. Additionally, capped RNA synthesized in transcription reactions is used for microinjection, in vitro translation, and transfection.
Learn More Directional TOPO cloning
Directional TOPO cloning enables cloning of blunt-ended PCR products in a 5´→3´ orientation directly into a expression vector using a 5-minute ligation reaction, thereby eliminating subcloning steps and saving you time.