5% somatic variant detection using Sanger sequencing
Applied Biosystems Minor Variant Finder Software is new software developed for the detection and reporting of minor mutations by Sanger sequencing.
Pan Ras Monoclonal Antibody (Ras10) (Invitrogen™)
TaqMan™ Mutation Detection Assay (castPCR) (Applied Biosystems™)
Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.
Somatic mutation detection
By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays provide:
• Higher specificity—designed to block the wild-type and amplify only the mutant
• Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
• Faster workflow—3 hours from sample to result
With 819 assays for 47 known cancer research genes available today like KRAS, BRAF, KIT, JAK2, the TaqMan Mutation Detection Assays provide an innovative tool for your cancer research.
All TaqMan Mutation Detection Assays have undergone extensive testing to ensure verify high sensitivity and specificity.