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eBook: Protein Quantification and Qualification Product Literature

When using a NanoDrop spectrophotometer, when do I use the autopathlength function in the UV-Vis module? Product FAQ

Answer

Selecting autopathlength should be considered if the peak of interest is greater than 1.25 A at a 1.0 mm pathlength or to ensure that the entire sample absorbance spectrum remains within the linear response of the detector. With autopathlength deselected, the instrument uses only a 1.0 mm pathlength which is optimized for absorbance signal less than 1.25 A.

Answer Id:: E17833

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When using a NanoDrop spectrophotometer, how does data collected in UV-Vis module compare to the data collected in another application? Product FAQ

Answer

UV-Vis data is reported at a normalized 1.0 mm pathlength. As a result, absorbance values reported here will be reported 10-fold lower than those reported by the Nucleic Acid or Protein A280 applications.

Answer Id:: E17834

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Acclaro Protein Contaminant ID Detection of Protein in Nucleic Acid Samples Using the NanoDrop One Spectrophotometer Product Literature

Brochure: NanoDrop One Microvolume UV-Vis Spectrophotometers Product Literature

How are your NanoDrop One/One<sup>c</sup> Spectrophotometers different from your NanoDrop 2000/2000c? Product FAQ

Answer

The NanoDrop One/Onec Spectrophotometers are the next generation instruments in the NanoDrop series of microvolume UV-Vis Spectrophotometers and will replace the NanoDrop2000/2000c Spectrophotometers. The NanoDrop One/Onec Spectrophotometers do everything that their predecessors can and more, including:
- on-board instrument control via a touch screen display
- a broader dynamic range (up to 27,500 ng/µL dsDNA)
- expanded connectivity and data management options
- enhanced sample analysis and technical support with Acclaro Sample Intelligence technology

Answer Id:: E17809

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Which pathlength was used for my sample measurement on the NanoDrop spectrophotometer pedestal? Product FAQ

Answer

The software is programmed to determine the best pathlength for the measurement based upon the absorbance signal of the sample at the analysis wavelength for the Nucleic Acids module, Protein A280 module, and Custom methods. On the other hand, the autopathlength function in the UV-Vis module monitors the entire wavelength range to optimize pathlength selection. The absorbance signal is normalized to a either 10 mm or 1.0 mm pathlength equivalent depending on module.

Answer Id:: E17835

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What pre-configured applications are included in my NanoDrop One/One<sup>c</sup> Spectrophotometers? Product FAQ

Answer

Listed below are the pre-configured applications included in our NanoDrop One/Onec Spectrophotometers:
- Nucleic Acid A260 (ds DNA, ssDNA, oligo DNA, oligo RNA, custom factor), A260/280, A260/230 and Microarray (labeled nucleic acids)
- Protein A280 and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Proteins and labels
- OD600
- Kinetics, UV-Vis and Custom Methods

Answer Id:: E17814

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Use of Custom Methods Capability of NanoDrop Spectrophotometers for Toxicology Studies Product Literature

Quality Control of Industrial Dye Products Using Absorbance Spectroscopy Product Literature

Application Note: Quantify Chlorophyll a And Chlorophyll b With A Custom Method Product Literature

Microvolume Quantification of Proteinsby UV-Vis Absorbance or Fluorescence Product Literature

Application Note: A Custom Method for Hemoglobin Measurements Product Literature

Application Note: Quantifying Glucose Concentration Using a Hexokinase Assay Product Literature

Brochure: RNA isolation and purification (North America) Product Literature

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