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A focused microarray to assess dopaminergic and glial cell differentiation from fetal tissue or embryonic stem cells. Citations & References

  • Authors: Luo Y, Schwartz C, Shin S, Zeng X, Chen N, Wang Y, Yu X, Rao MS
  • Journal: Stem Cells 2006; (24):4 865-875
  • PubMed ID: 16357341

Perivascular cells with pericyte characteristics are involved in ATP- and PGE(2)-induced relaxation of porcine retinal arterioles in vitro. Citations & References

  • Authors: Misfeldt MW, Pedersen SM, Bek T,
  • Journal: Invest Ophthalmol Vis Sci (2013) 54:3258-3264
  • PubMed ID: 23599323
Catalog # S11364

The glial cell glutamate uptake carrier countertransports pH-changing anions. Citations & References

  • Authors: Bouvier M, Szatkowski M, Amato A, Attwell D
  • Journal: Nature (1992) 360:471-474
  • PubMed ID: 1448171
Catalog #

Immunoglobulins stimulate cultured Schwann cell maturation and promote their potential to induce axonal outgrowth. Citations & References

  • Authors: Tzekova N, Heinen A, Bunk S, Hermann C, Hartung HP, Reipert B, Küry P,
  • Journal: J Neuroinflammation (2015) 12:107-107
  • PubMed ID: 26022648
Catalog # KRC2341

Co-transplantation of GDNF-overexpressing neural stem cells and fetal dopaminergic neurons mitigates motor symptoms in a rat model of Parkinson's disease. Citations & References

  • Authors: Deng X, Liang Y, Lu H, Yang Z, Liu R, Wang J, Song X, Long J, Li Y, Lei D, Feng Z
  • Journal: PLoS One 2013; (8):12 e80880-e80880
  • PubMed ID: 24312503
Catalog # 87792

Unexpected expression of alpha- and beta-globin in mesencephalic dopaminergic neurons and glial cells. Citations & References

  • Authors: Biagioli M, Pinto M, Cesselli D, Zaninello M, Lazarevic D, Roncaglia P, Simone R, Vlachouli C, Plessy C, Bertin N, Beltrami A, Kobayashi K, Gallo V, Santoro C, Ferrer I, Rivella S, Beltrami CA, Carninci P, Raviola E, Gustincich S
  • Journal: Proc Natl Acad Sci U S A 2009; (106):36 15454-15459
  • PubMed ID: 19717439

Identification of novel glial genes by single-cell transcriptional profiling of Bergmann glial cells from mouse cerebellum. Citations & References

  • Authors: Koirala S, Corfas G
  • Journal: PLoS One 2010; (5):2 e9198-e9198
  • PubMed ID: 20169146

Genome-wide analysis of Müller glial differentiation reveals a requirement for Notch signaling in postmitotic cells to maintain the glial fate. Citations & References

  • Authors: Nelson BR, Ueki Y, Reardon S, Karl MO, Georgi S, Hartman BH, Lamba DA, Reh TA
  • Journal: PLoS One 2011; (6):8 e22817-e22817
  • PubMed ID: 21829655

The transcriptome of retinal Müller glial cells. Citations & References

  • Authors: Roesch K, Jadhav AP, Trimarchi JM, Stadler MB, Roska B, Sun BB, Cepko CL
  • Journal: J Comp Neurol 2008; (509):2 225-238
  • PubMed ID: 18465787

Characterization of a cell surface adhesion molecule expressed by a subset of developing chick neurons. Citations & References

  • Authors: el-Deeb S, Thompson SC, Covault J
  • Journal: Dev Biol (1992) 149:213-227
  • PubMed ID: 1728591
Catalog # M8997(Blocked)

Binding between the neural cell adhesion molecules axonin-1 and Nr-CAM/Bravo is involved in neuron-glia interaction. Citations & References

  • Authors: Suter DM, Pollerberg GE, Buchstaller A, Giger RJ, Dreyer WJ, Sonderegger P
  • Journal: J Cell Biol (1995) 131:1067-1081
  • PubMed ID: 7490283
Catalog # M8997(Blocked)

Identification of membrane proteins that comprise the plasmalemmal junction between migrating neurons and radial glial cells. Citations & References

  • Authors: Cameron RS, Rakic P
  • Journal: J Neurosci (1994) 14:3139-3155
  • PubMed ID: 8182462
Catalog # R415

Expression of cone-like properties by chick embryo neural retina cells in glial-free monolayer cultures. Citations & References

  • Authors: Adler R, Lindsey JD, Elsner CL
  • Journal: J Cell Biol (1984) 99:1173-1178
  • PubMed ID: 6470040
Catalog # C827

Parkinson disease-associated DJ-1 is required for the expression of the glial cell line-derived neurotrophic factor receptor RET in human neuroblastoma cells. Citations & References

  • Authors: Foti R, Zucchelli S, Biagioli M, Roncaglia P, Vilotti S, Calligaris R, Krmac H, Girardini JE, Del Sal G, Gustincich S
  • Journal: J Biol Chem 2010; (285):24 18565-18574
  • PubMed ID: 20395301

Why are there no viable primary neuron cells/astrocytes/rat glial precursor cells after thawing the cell stock? Product FAQ

Answer

These cells are very fragile. We recommend that you follow the procedure in the manual and use the correct medium. Fast thawing is the key for healthy culture. There are several critical points to consider:

- Pre-rinse all materials with medium before use. Do not use PBS, DPBS, or HBSS to rinse because they do not contain proteins.
- Thaw cells quickly and do not expose cells to air.
- Transfer cells to a pre-rinsed tube first, then add slowly add medium in a drop-wise manner. Do not add the full amount of medium to the cells at once because this may lead to decreased cell viability due to osmotic shock.
- Use pre-warmed complete growth medium and correct seeding density.
- Matrix coating is required for some cell cultures.
- For primary neuron cells, do not centrifuge the cells as they are extremely fragile upon recovery from cryopreservation.

Answer Id:: E12050

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