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Methods and reagents – Carriers for precipitating nucleic acids Citations & References

  • Authors: P. N. Hengen.
  • Journal: TIBS
  • PubMed ID: 0

What is different about the MagMAX CORE Nucleic Acid Purification Kit compared to the other MagMAX kits? Product FAQ

Answer

The MagMAX CORE Nucleic Acid Purification Kit offers pre-mixed, room-temperature reagents, which greatly improve product handling and storage. Specifically, the CORE kit decreases processing times by having pre-mixed washes that do not require the addition of alcohol, a single protocol adapted on multiple KingFisher magnetic particle processors, and a complete protocol that addresses 14 unique sample types.

Answer Id:: E16164

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Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids. Citations & References

  • Authors: Markovits J, Roques BP, Le Pecq JB
  • Journal: Anal Biochem
  • PubMed ID: 464292
Catalog #

Could you provide an overview of the ULYSIS Nucleic Acid Labeling Kits? Product FAQ

Answer

The ULYSIS oligonucleotide chemical labeling reagents allow you to rapidly and easily couple our fluorescent dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), is based on the use of a platinum coordination complex that forms a stable adduct at the N7 position of guanine. ULYSIS labeled probes are used in fluorescence in situ hybridization (FISH) and numerous other applications.

Answer Id:: E8090

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Is propidium iodide (PI) fixable with glutaraldehyde or paraformaldehyde (PFA)? Product FAQ

Answer

PI is not fixable with glutaraldehyde or PFA. Both reagents fix by crosslinking amines. PI and other nucleic acid stains do not inherently bind covalently to nucleic acids and these fixatives do not crosslink the dyes to nucleic acids.
The one fixable nucleic acid stain is Ethidium Monoazide Bromide (EMA), Cat no. E1374); it covalently binds to nucleic acids upon activation by exposure to light.

Answer Id:: E18024

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How many samples can I prepare with the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay? Product FAQ

Answer

The Oncomine Lung Cell-Free Total Nucleic Acid Research Assay contains sufficient reagents to prepare 8 libraries from cell-free total nucleic acid.

Answer Id:: E17072

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Efficient gene silencing by delivery of locked nucleic acid antisense oligonucleotides, unassisted by transfection reagents. Citations & References

  • Authors: Stein CA, Hansen JB, Lai J, Wu S, Voskresenskiy A, Hog A, Worm J, Hedtjarn M, Souleimanian N, Miller P, Soifer HS, Castanotto D, Benimetskaya L, Orum H, Koch T
  • Journal: Nucleic Acids Res
  • PubMed ID: 19854938

Dendritic nucleic acid structures. Citations & References

  • Authors: Nilsen TW, Grayzel J, Prensky W
  • Journal: J Theor Biol
  • PubMed ID: 9237897
Catalog #
  • U24832
  • C29031

Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism. Citations & References

  • Authors: Pin S, Chen H, Lein PJ, Wang MM
  • Journal: J Neurochem
  • PubMed ID: 16441515
Catalog #

Denaturation of nucleic acids induced by intercalating agents. Biochemical and biophysical properties of acridine orange-DNA complexes. Citations & References

  • Authors: Kapuscinski J, Darzynkiewicz Z
  • Journal: J Biomol Struct Dyn
  • PubMed ID: 6400830
Catalog #

[Real time PCR methodology for quantification of nucleic acids] Citations & References

  • Authors: Tse C, Capeau J
  • Journal: Ann Biol Clin (Paris)
  • PubMed ID: 12805005
Catalog #

Stimulation of proteinase K action by denaturing agents: application to the isolation of nucleic acids and the degradation of “masked” proteins Citations & References

  • Authors: H. Hilz et al.
  • Journal: Eur J Biochem
  • PubMed ID: 0

How does TRIzol Reagent work for RNA isolation? Product FAQ

Answer

TRIzol Reagent contains phenol and guanidine isothiocyanate, which allow for isolation of nucleic acids from proteins. Further partitioning of the nucleic acids occurs in a pH dependent manner. At pH 7.0 or higher, DNA and RNA partition into the aqueous phase. At an acidic pH, below 7.0, DNA is denatured and will move into the organic phase (interphase) yet the RNA remains in the aqueous phase.TRIzol Reagent has pH about 5.

Answer Id:: E4541

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Competitive PCR for precise nucleic acid quantification Citations & References

  • Authors: Zentilin, L; Giacca, M
  • Journal: Nature Protocols
Catalog #
  • 4318739(Discontinued)
  • 4327059(Discontinued)
  • 4327058(Discontinued)

The Pluripotent Stem Cell Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain? Product FAQ

Answer

If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

Answer Id:: E19006

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