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SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

SYTO™ 83 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 83 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

SYTO™ 84 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 84 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Invitrogen™)

SYTO 9 green fluorescent nucleic acid stain has been shown to stain live and dead Gram-positive and Gram-negative bacteria, and it is a component of the LIVE/DEAD BacLight Bacterial Viability Kits (L-7007, L-7012, L-13152).

SYTO™ 85 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 85 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

SYTO™ BC Green Fluorescent Nucleic Acid Stain (Invitrogen™)

SYTO BC green fluorescent nucleic acid stain is a mixture of some of our best SYTO dyes for bacterial staining and is a component of the Bacteria Counting Kit (B-7277).

SYTO™ 17 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 17 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

SYTOX Orange dye stains nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide (P-1304).

SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (S-11350) to enable researchers to find the dye that works best for a particular application.

SYTO™ 13 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 13 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTO™ Green Fluorescent Nucleic Acid Stain Sampler Kit #1 - SYTO™ dyes 11-16 - 50 µL each (Invitrogen™)

The SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 contains our collection of cell-permeant, green-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL each of SYTO 11—16 dyes.

SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 41 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (S-11350) to enable researchers to find the dye that works best for a particular application.

SYTO™ 14 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 14 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

SYTOX® Blue nucleic acid stain is an excellent blue-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. The excitation/emission maxima for the dye bound to DNA are 444/480 nm.

SYTO™ 11 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 11 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells (Invitrogen™)

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells. With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration.

Features of SYTOX Deep Red Nucleic Acid Stain include:
• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers

SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used. SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers.

SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria. It is supplied at a 1 mM concentration in DMSO (~2000X concentration). A concentration of 0.5 µM (1X) is recommended for most applications and cell types. In fluorescent imaging of various mammalian cells, a concentration range of 2.5 µM to 0.25 µM has been used. For viability testing by flow cytometry, a concentration of 1 µM to 10 nM has been used for Jurkat cells and a range of 4 µM to 0.5 µM has been used with E. coli.

Nucleic Acid Stains Dimer Sampler Kit (Invitrogen™)

The Nucleic Acid Stains Dimer Sampler Kit contains 25 µL each of the following high-affinity nucleic acid stains based upon symmetric dimers of cyanine dyes: BOBO-1 iodide (Cat. No. B3582), BOBO-3 iodide (B3586), POPO-1 iodide (P3580), POPO-3 (P3584), TOTO-1 iodide (T3600), TOTO-3 iodide (T3604), YOYO-1 iodide (Y3601), and YOYO-3 iodide (Y3603).

SYTO™ 21 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 21 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #2 (S-7554) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTO™ 59 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 59 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

SYTO™ Orange Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 80-85 - 50 µL each (Invitrogen™)

The SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit contains our collection of cell-permeant, orange-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL each of SYTO 80—85 dyes.

SYTO™ 16 Green Fluorescent Nucleic Acid Stain - 1 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 16 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45) (Invitrogen™)

The SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit contains a collection of cell-permeant, blue-fluorescent SYTO nucleic acid stains (SYTO® dyes 40, 41, 42, 45). Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL of each SYTO dye.

SYTO™ 12 Green Fluorescent Nucleic Acid Stain - 4 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 12 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTO™ Deep Red Nucleic Acid Stain, for live cells (Invitrogen™)

SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells. It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments. After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration.

• Cell-permeant dye that stains nuclei of live, dead, or fixed cells
• Detectable with deep red/Cy5 fluorecence filter set, with excitation/emission maxima of 652/669 nm
• Five times greater fluorescent signal when staining dsDNA vs RNA

SYTO Deep Red Nucleic Acid Stain can be mulitplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used. The stain increases in fluorescence with increasing concentrations of dsDNA. SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers. The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.

SYTO™ 64 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 64 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population.
 Impermeant to live cells
 Excitation/Emission: 504/523 nm
 Use with 488 Argon-ion laser
 Works with mammalian cells and both Gram-positive and Gram-negative bacteria

Simple Viability Determination
SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, but will easily penetrate compromised membranes characteristic of dead cells. Because it exhibits >500-fold fluorescence enhancement upon binding nucleic acids, briefly incubating dead cells with SYTOX® Green will result in bright green fluorescence with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450–490 nm source. The exceptional brightness of the signal produced in dead cells makes SYTOX® Green particularly useful for determining viability of not just mammalian cells, but both Gram-positive and Gram-negative bacteria.

Part of the Invitrogen Family of Cell Viability Stains and Assays
SYTOX® stains for dead cells are available in a variety of colors, including red, blue, and orange. Additionally, Invitrogen has incorporated SYTOX® stains into a number of assays for apoptosis, cell viability, and metabolism.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Find out more about all SYTOX® Products.

SYTO™ Red Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 17 and 59-64 - 50 µL each (Invitrogen™)

The SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit contains our collection of cell-permeant, red-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL each of SYTO 17 and 59—64 dyes.

SYTO™ 24 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 24 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #2 (S-7554) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

SYTO™ 63 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 63 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

SYTO™ 62 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 62 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.