A three-part series of review articles that helps to clarify these issues has recently been published in Current Protocols in Cytometry. Authored by our R&D scientists Jason Kilgore and Nick Dolman, who have developed and tested many Molecular Probes® organelle stains, as well as imaging guru Michael Davidson of Florida State University, this series pulls together a wealth of references and personal knowledge that covers the most common fluorescent reagents for nearly every cellular structure or organelle, focusing primarily on microscopic analysis. Each article is broken into sections by target structure or organelle. The discussion includes a short history on the different reagents that have been used and their characteristics. A featured reagent is highlighted, along with a validated protocol for that reagent with an estimate on protocol time, a troubleshooting guide in table format, an image example, and relevant recipes for stock and final solutions.
Learn More Probes for Visualizing Secretory Transport and Autophagy: Illuminating Organelle Dynamics in Live Cells
Examining cellular pathways in both their spatial and temporal context is critical for understanding cell development and functioning. Molecular \r\nProbes® CellLight™ reagents provide a simple and effective method for introducing targeted intracellular labels into live cells, enabling \r\nunambiguous visualization of organelles and other cellular structures in live mammalian cells by fluorescence microscopy. The secretory \r\npathway involves a series of complex and highly regulated steps that ultimately leads to the delivery of proteins out of the cell. The endoplasmic reticulum (ER) and Golgi apparatus are the key membrane-bound intracellular compartments involved in the processing and posttranslational modification of proteins prior to their delivery. These compartments exist in a dynamic equilibrium mediated by anterograde traffic from the ER to the Golgi, and reciprocal retrograde trafficking from the Golgi to the ER. This transport is mediated by small GTPases, which participate in the budding of transport vesicles. Similar processes govern lysosome-mediated organelle degradation (autophagy). When CellLight™ reagents are combined with Molecular Probes® classic organelle stains, these dynamic organelle processes can be studied in their native cellular context.\r\n
Learn More FilmTracer Biofilm Stains
Our FilmTracer biofilm stains enable the observation of cells in the context of their EPS.
Learn More Secondary Antibodies
Secondary antibodies significant advantages are increased sensitivity through the signal amplification and greater flexibility in labeling and detection options.
Learn More Structured Illumination Microscopy (SIM)
Find Molecular Probes® fluorescence labels for the generation of structured illumination microscopy (SIM) images, useful for thicker sections and for 3D and live-cell imaging.
Learn More Fluorescent Secondary Antibodies
Fluorescent-dye conjugated secondary antibodies provide a much needed tool for identifying proteins in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more.
Learn More Fluorescence Microscopy Reagents & Assays
Getting publication-quality images and data is critical to every researcher. Thermo Fisher Scientific offers the highest quality fluorescence microscopy reagents and assays including cell structure and function dyes and stains, antifades and mountants, instrument calibration and set-up tools and much more.
Learn More Imaging Cell Structure
Find high-quality Molecular Probes® organelle-specific stains and antibody conjugates for fluorescence imaging of live and fixed cells and tissues.
Learn More HCS Toolbox Reagents for Image Segmentation
Image segmentation is a key aspect of image-based high-content screening (HCS) assays and is the first step of automated image acquisition and analysis. Image segmentation involves separating objects of interest (cells) from background or other features not relevant to the analysis and allows determination of items of interest in their appropriate spatial context such as translocation of biomarkers or changes in biomarker levels within particular cellular compartments. Key approaches for image segmentation involve using fluorescent stains selective for specific parts of the cell—nucleus, cytoplasm, plasma membrane, or other organelles— to delineate the whole cell. Invitrogen's Molecular Probes® fluorescent stains and dyes are validated for high-content analysis and provide you with the tools you need for accurate cell analysis. Please read further for products for nuclear image segmentation, cytoplasm image segmentation, plasma membrane image segmentation or organelle image segmentation.
Learn More Alexa Fluor Plus Secondary Antibodies
A new family of 4x brighter fluorescent secondary antibody conjugates based on Alexa Fluor dyes 488, 555, 594, 647, 680, and 800.
Learn More Cell Imaging Reagent Guide and Protocols App
The Molecular Probes® Cell Imaging Reagent Guide & Protocols App. This mobile app is designed to help you find fluorescent dyes, reagents and protocols for cell biology related fluorescence microscopy applications. Available for iPhone, iPod touch, and Android devices.
Here we highlight a few of the most-referenced Molecular Probes reagents for investigating mitochondria in both live- and fixed-cell imaging applications.
Learn More Integrin Cell Signaling Pathway
Interactions between integrins and the extracellular matrix mediate intracellular signaling mechanisms to regulate cell survival, differentiation, cell cycle progression, and inflammatory responses.
Learn More Alexa Fluor Secondary Antibodies
Alexa Fluor conjugated secondary antibodies exhibit superior brightness and photostability, outperforming conventional fluorescent secondary antibodies.
Learn More Plasma Membrane
Find fluorescent reagents for plasma membrane staining in live and fixed cells.