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Analysis and identification of XPS spectra of organic polymer materials Product Literature

What is the basic structure of a Qdot nanocrystal? Product FAQ

Answer

A Qdot nanocrystal is comprises four basic layers. Listed from inner core to outer shell, these are:

1) Core nanocrystal (CdSe or CdSeTe): Determines the color of the Qdot nanocrystal
2) Inorganic shell (ZnS): Improves brightness and stability of the Qdot nanocrystal
3) Organic/polymer coating: Provides water solubility and/or functional groups for conjugation
4) Biomolecule: Covalently attached to the polymer shell and can include antibodies, streptavidin, receptor ligands, or oligonucleotides.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E12217

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How do Phasemaker Tubes work? Product FAQ

Answer

Phasemaker Tubes work on the principle of density gradient centrifugation. Phasemaker Tubes contain a thick liquid polymer that is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation, the polymer positions itself between these two layers and forms an exclusive physical barrier. It separates and completely isolates the upper aqueous phase from the organic phase underneath, making it very easy to aspirate the upper phase containing the RNA.

Answer Id: E15197

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Are Dynabeads magnetic beads compatible with fluorescence activated cell sorting (FACS)? Product FAQ

Answer

All Dynabeads magnetic beads show some degree of autofluorescence. The polymer core matrix, the organic chemistry used on the surface, and the magnetic oxides contribute to the autofluorescence. When excited at 488 nm using an argon laser, the beads emit both green and red fluorescence that overlaps the fluorescence from FITC and PE, respectively. This will make the interpretation of fluorescence histograms difficult or, in some cases, impossible.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E6203

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How does the protocol for staining with Super Bright-conjugated antibodies differ from staining with conventional organic fluorochrome and eVolve conjugated antibodies? Product FAQ

Answer

Antibodies conjugated to Super Bright dyes can be used similarly to traditional fluorochromes. If multiple Super Bright dyes are used in combination with each other or in combination with other polymer dyes (i.e., Brilliant Violet dyes), then the use of Super Bright Staining Buffer (Cat. No. SB-4400) is recommended to minimize any non-specific polymer interactions between these fluorochromes. For specific instructions for use, please refer to the product technical data sheet.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14679

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What is the difference between an ITK Qdot nanocrystal product and a standard Qdot nanocrystal product? Product FAQ

Answer

ITK Qdot nanocrystals use the original formulation of outer polymer provided in the first generation of the Qdot products; except for the Amine-PEG products, the outer polymer does not include PEG. The outer polymer of the standard Qdot nanocrystals includes PEG.

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Answer Id: E12228

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What is the temperature range in which Qdot nanocrystals are stable? Product FAQ

Answer

When stored at 4 degrees C, Qdot nanocrystals are stable for approximately 6 months. Qdot nanocrystals should never be frozen due to the possibility of aggregation. The temperature stability of Qdot nanocrystals is summarized below. Please note that fluorescence is not temperature dependent.

<0 degrees C: NEVER freeze Qdot nanocrystals - polymer induces aggregation at freezing temperatures.
>4 degrees C: Core/Shell/Polymer stable at 4 degrees C for ~ 6 months. May be filter sterilized using uncharged filters.
<60 degrees C: Core/Shell/Polymer stable at 60 degrees C (as in in situ hybridization).
<65 degrees C: Core/Shell/Polymer stable at 65 degrees C for only ~1 hour, beyond 1 hour, emission drops off.
<100 degrees C: Core/Shell/Polymer stable up to 100 degrees C brief exposure. OK for 5 minutes at 100 degrees C.
<360 degrees C: Only Core/Shell stable up to 360 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E12223

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How large are the Qdot nanocrystals? Product FAQ

Answer

The core/shells are only a few nanometers in diameter (some are elliptical), but with the outer polymer coatings, a fully-functionalized Qdot nanocrystal can range from 15 to 21 nm in hydrodynamic diameter, similar in size to some proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E6496

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Chelators, Calibration Buffers, Ionophores and Cell-Loading Reagents—Section 19.8 Molecular Probes Handbook

FT-IRラマンサンプリングアクセサリガイド Product Literature

Application Note: Accelerated Solvent Extraction of Plasticizers from Polyvinyl Chloride Polymer Product Literature

What is the pH range in which Qdot nanocrystals are stable? Product FAQ

Answer

Qdot nanocrystals are most stable at pH 6-9, and marginal stability of Qdot nanocrystals is shown down to a pH 5. Qdot nanocrystals should not be used at pH > 9 due to the possibility of self-aggregation and clumping, and Qdot nanocrystals should not be used pH less than 4 as the polymer and exposed core/shell will begin to dissociate. For more information on Qdot nanocrystals and recommended pH ranges, see pH Ranges for Qdot Nanocrystals (https://www.thermofisher.com/us/en/home/brands/molecular-probes/key-molecular-probes-products/qdot/qdot-reg--nanocrystal0.html)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E12222

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Organic-matter degradative potential of Halomonas glaciei isolated from frazil ice in the Ross Sea (Antarctica) Citations & References

  • Authors: Celussi, M; Balestra, C; Fabbro, C; Crevatin, E; Cataletto, B; Umani, SF; Del Negro, P
  • Journal: FEMS MICROBIOLOGY ECOLOGY

Multiplexed imaging of nucleome architectures in single cells of mammalian tissue. Citations & References

  • Authors: Liu M, Lu Y, Yang B, Chen Y, Radda JSD, Hu M, Katz SG, Wang S
  • Journal: Nat Commun
  • PubMed ID: 32518300
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Brochure: Sensititre Standard Formularies List (U.S.A. Only) Product Literature

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