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How long is the control PCR fragment supplied in the InsTAclone PCR Cloning kits (Cat. Nos. K1213, K1214)? Product FAQ

Answer

The control PCR fragment is 953 bp long.

Answer Id:: E13234

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What is the difference between the TaqMan Universal PCR Master Mix Cat. No. 4304437 and Cat. No. 4324018? Product FAQ

Answer

Both part numbers for TaqMan Universal PCR Master Mix are supplied at a 2X concentration and contain the following components: AmpliTaq Gold DNA polymerase, dNTPs with dUTP, Passive Reference (ROX), and optimized buffer components. However, the part number 4304437 also contains AmpErase UNG and is not suitable to be used for One Step RT PCR.

Answer Id:: E1581

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pCR8/GW/TOPO TA Cloning Kits QRC (Supply Centers) Manual / Product Insert

  • Version: 20 April 2012
Catalog #

Polymerase chain reaction and gene probe detection of the 2,4-dichlorophenoxyacetic acid degradation plasmid, pJP4. Citations & References

  • Authors: Neilson JW; Josephson KL; Pillai SD; Pepper IL
  • Journal: Applied and Environmental Microbiology
Catalog #

Can I purchase just the 10X PCR buffer that comes with Platinum Taq? Product FAQ

Answer

The 10X PCR buffer for Platinum Taq is not available as a stand-alone item. It is only supplied as part of the enzyme kit.

Answer Id:: E7625

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How are IonCode Barcode Adapters, 1153-1536 supplied? Product FAQ

Answer

IonCode Barcode Adapters, 1153-1536 are supplied in 4 separate 96-well plates containing 25 µL of each barcode:
- IonCode Barcode Adapters 1301-1396 in 96-well PCR plate (red)
- IonCode Barcode Adapters 1401-1496 in 96-well PCR plate (yellow)
- IonCode Barcode Adapters 1501-1596 in 96-well PCR plate (green)
- IonCode Barcode Adapters 1601-1696 in 96-well PCR plate (blue)

Answer Id:: E18653

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Can I connect several QuantStudio 1 instruments to the same computer? Product FAQ

Answer

Yes, it is possible. Please use local area network (LAN) connection and see page 17 of the QuantStudio 1 Real-Time PCR System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017853_QS1%20UG.pdf) for supported configuration options. However, it is not possible to connect more than one instrument directly to one computer (supplied by Thermo Fisher Scientific) using wired connection due to single Ethernet port availability on the computer supplied by us. Also, please note that starting a run simultaneously from the same computer on two connected instruments is not yet fully validated.

Answer Id:: E18831

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Efficacy of filter types for detecting Campylobacter jejuni and Campylobacter coli in environmental water samples by polymerase chain reaction. Citations & References

  • Authors: Oyofo BA; Rollins DM
  • Journal: Applied and Environmental Microbiology
Catalog #

What concentration is the vector pTZ57R/T supplied at in the InsTAclone PCR Cloning kits? Product FAQ

Answer

The vector pTZ57R/T is supplied at 55 ng/µL.

Answer Id:: E13233

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Temperature mediates vector transmission efficiency: inoculum supply and plant infection dynamics Citations & References

  • Authors: Daugherty, MP; Bosco, D; Almeida, RPP
  • Journal: ANNALS OF APPLIED BIOLOGY
Catalog #

How does TA Cloning work? Product FAQ

Answer

Taq polymerase has a non-template-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3´ ends of PCR products. The linearized vector supplied in our TA Cloning kits have single, overhanging 3´ deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Answer Id:: E4061

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With the GeneArt High-Order Genetic Assembly System, I'm getting no positive colonies detected by yeast colony PCR. Can you please offer some troubleshooting tips? Product FAQ

Answer

We would recommend trying to re-streak the colony on a fresh plate and repeat colony PCR. Do not break open the yeast cells with the beads supplied with the kit; the beads are for transformation into E. coli. Additionally, use less than 0.5 µL of diluted yeast lysate in a 50 µL PCR reaction.

Answer Id:: E6862

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Free-Living Protozoa in Two Unchlorinated Drinking Water Supplies, Identified by Phylogenic Analysis of 18S rRNA Gene Sequences Citations & References

  • Authors: Valster, RM; Wullings, BA; Bakker, G; Smidt, H; van der Kooij, D
  • Journal: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Catalog #
  • 4339386
  • 4413750
  • N8050400(Discontinued)
  • N8050200(Discontinued)
  • N8050002(Discontinued)
  • ZGEXSC9700384W
  • ZGEXSC9700384W3Y
  • N8050002R
  • 4441166

100023351 SDS

Catalog #

Comparison of two molecular methods used for subtyping of Legionella pneumophila 1 strains isolated from a hospital water supply Citations & References

  • Authors: Casini, B; Valentini, P; Baggiani, A; Torracca, F; Lorenzini, C; Frateschi, S; Matteoli, B; Privitera, G
  • Journal: WATER SCIENCE AND TECHNOLOGY
Catalog #
  • 4339386
  • 4413750
  • N8050400(Discontinued)
  • N8050200(Discontinued)
  • N8050002(Discontinued)
  • ZGEXSC9700384W
  • ZGEXSC9700384W3Y
  • N8050002R
  • 4441166
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