Documents & Support

Could you suggest references for cDNA libraries and RT-PCR using Dynabeads magnetic beads? Product FAQ

Answer

These are some references on cDNA libraries and RT-PCR:

Jakobsen KS, Haugen M, Sæbøe-Larssen S, Hollung K, Espelund M, Hornes E. Direct mRNA isolation using Magnetic Oligo (dT) Beads: A protocol for all types of cell cultures, animal and plant tissues. In Advances in Biomagnetic Separation, Ed Uhlén, M., Hornes, E., Olsvik Ø., Eaton Publishing. 1994:61-72

Raineri I, Moroni C, Senn HP. Improved efficiency for single-sided PCR by creating a reusable pool of first-strand cDNA coupled to a solid phase. Nucleic Acids Research 1991;19:4010

Raineri I, Senn HP. HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts. Nucleic Acids Res. 1992;20:6261-6266

Sharma P, Lönneborg A, Stougaard P. PCR-based construction of subtractive cDNA library using magnetic beads. BioTechniques 1993;15:610-611

Lee Y-H, Vacquier VD. Reusable cDNA libraries coupled to magnetic beads. Anal. Biochem. 1992;206:206-207

Lambert KN, Williamson VM. cDNA library construction from small amounts of RNA using paramagnetic beads and PCR. Nucleic Acids Research 1993;21:775-776

Aasheim H-C, Deggerdal A, Smeland EB, Hornes E. A simple subtraction method for the isolation of cell- specific genes using magnetic monodisperse polymer particles. BioTechniques 1994;16:716-721

Coche T, Dewez M, Beckers M-C. Generation of an unlimited supply of a subtracted probe using magnetic beads and PCR. Nucleic Acid Research 1994;22:1322-1323

Rodriguez IR, Chader GJ. A novel method for the isolation of tissue-specific genes. Nucleic Acids Research 1992;20:3528

Schraml P, Shipman R, Stulz P, Ludwig CU. cDNA subtraction library construction using a magnet-assisted subtraction technique (MAST). Trends in Genetics 1993;3:70-71

Wada H, Asada M, Miyazaki M, Ilda S, Mizutani S. Application of oligo (dT) Dynabeads for the molecular diagnosis of human leukemia. The John Uglestad Conference I: Magnetic separation techniques applied to cellular and molecular biology, 1991

Larsen F, Solheim J, Kristensen T, Kolstø AB, Prydz H. A tight cluster of five unrelated human genes on chromosome 16q22.1. Human Molecular Genetics 1993;2:1589-1595

Answer Id:: E6181

Was this answer helpful?

Yes No

Thank you for your response

What is included in the Ion AmpliSeq Kit for Chef DL8? Do I have to get any additional reagents? Product FAQ

Answer

The Ion AmpliSeq Kit for Chef DL8 enables robust, automated Ion AmpliSeq library preparation from genomic DNA or RNA using the Ion Chef Instrument and 1- or 2-pool Ion AmpliSeq primer panels. The kit contains sufficient reagents and consumables to prepare up to 32 barcoded and equalized libraries that are ready for template preparation using either the Ion Chef System or the Ion OneTouch 2 System. Library preparation from RNA additionally requires the SuperScript VILO cDNA Synthesis Kit, ordered separately. The kit components are as follows:

- Ion AmpliSeq Chef Reagents DL8 (4 cartridges)
- Ion AmpliSeq Chef Solutions DL8 (4 cartridges)
- Ion AmpliSeq Chef Supplies DL8 (1 box with 4 inserts)
Per insert:
-- Ion AmpliSeq Tip Cartridge DL8
-- Frames PCR Foil Seal
-- Enrichment Cartridge

- IonCode 0101-0132 in 96-well PCR Plates (dried) (1 set of 4 plates):
- IonCode 0101-0108 in 96 Well PCR Plate (red)
- IonCode 0109-0116 in 96 Well PCR Plate (yellow)
- IonCode 0117-0124 in 96 Well PCR Plate (green)
- IonCode 0125-0132 in 96 Well PCR Plate (blue)

Answer Id:: E11505

Was this answer helpful?

Yes No

Thank you for your response

What is the difference between the regular BigDye (Terminator) kits and the BigDye Direct Cycle Sequencing Kit? Product FAQ

Answer

BigDye Direct Cycle Sequencing Kit supplies reagents for the complete cycle sequencing workflow, including the PCR reaction, reaction cleanup, and sequencing the PCR product. The post-PCR and cycle sequencing process is combined into a single step, and the workflow requires that the initial PCR primers be designed with an M13 tail, since M13 primers will be used for the sequencing reaction. BigDye Terminator Cycle Sequencing Kits are designed so that the initial PCR and the cleanup are performed as separate reactions. This allows the user more flexibility in primer design and the option to break up the workflow. For the comparative studies that we performed in-house, we found sequencing data obtained using these two kits to be concordant and of at least equivalent quality (typically the BigDye Direct Cycle Sequencing Kit produced better results).

Answer Id:: E6278

Was this answer helpful?

Yes No

Thank you for your response

3D Cell Culture Handbook Product Literature

Supplemental Instructions: VetMAX C. burnetii Feces Kit (English) Manual / Product Insert

  • Version: A.0
Catalog # FQPE

Protocol: ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit: Protocol (English ) Manual / Product Insert

  • Version: 23 Jul 2010
Catalog #

The first documented case of Aspergillus cardiac surgical site infection in Sweden: an epidemiology study using arbitrarily primed PCR Citations & References

  • Authors: Bergman, A; Lignell, A; Melhus, A
  • Journal: APMIS 2009 8:568-574
Catalog #
  • 4339386
  • 4413750
  • N8050002(Discontinued)
  • N8050200(Discontinued)
  • N8050400(Discontinued)
  • 4441166
  • N8050002R
  • ZGEXSC9700384W
  • ZGEXSC9700384W3Y

The defined combination of growth factors controls generation of long-term-replicating islet progenitor-like cells from cultures of adult mouse pancreas. Citations & References

  • Authors: Ta M; Choi Y; Atouf F; Park CH; Lumelsky N
  • Journal: Stem Cells (Dayton, Ohio) 2006 7:1738-1749
Catalog #

User Guide: Axiom Propel XPRES 384HT Workflow Manual / Product Insert

  • Version: B.0

Triple Combination of Oseltamivir, Amantadine, and Ribavirin Displays Synergistic Activity against Multiple Influenza Virus Strains In Vitro Citations & References

  • Authors: Nguyen, JT; Hoopes, JD; Smee, DF; Prichard, MN; Driebe, EM; Engelthaler, DM; Le, MH; Keim, PS; Spence, RP; Went, GT
  • Journal: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 2009 10:4115-4126
Catalog #

Catalog: NCC Cancer Therapeutic - Aug 2020 - Singapore Product Literature

User Guide: QuantStudio 3D Digital PCR System Manual / Product Insert

  • Version: E.0
Catalog #

Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve. Citations & References

  • Authors: Kummer W; Shigemoto R; Haberberger R
  • Journal: Neuroscience Letters 1999 2:119-122
Catalog #
  • 4318739(Discontinued)
  • 4327058(Discontinued)
  • 4327059(Discontinued)
Results per page
    spinner