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PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for IF, ICC, IHC (P)

LanthaScreen™ PDCD4 HEK 293E Cell Line

The PI3K⁄AKT⁄mTOR pathway mediates signals for cell growth and survival, transcription and translation, and regulated cell death. The significance of mTOR has been underscored recently by the identification of two distinct cellular complexes: mTORC1 (includes raptor and is rapamycin sensitive) and mTORC2 (includes rictor and is insensitive to rapamycin). Activation of the pathway by insulin (or other mitogens) leads to the phosphorylation of programmed cell death protein 4 (PDCD4) via mTORC1 (indirectly through the ribosomal protein p70 S6 kinase). LanthaScreen® PDCD4 HEK293E is a human cell line which constitutively expresses GFP-PDCD4 fusion proteins. This kinase target was introduced using lipid transfection followed by selection with Blasticidin. These cells are a clonal population isolated by FACS using GFP fluorescence as a sorting marker. A homogenous immunoassay ("addition only", no wash steps) was developed in 384-well format using a TR FRET-based detection method to monitor the phosphorylation of Ser457 on GFP-PDCD4 using a terbium-labeled phosphospecific antibody. The assay has been optimized for performance under numerous experimental conditions (e.g., cell plating density, DMSO tolerance, insulin stimulation time, etc.) and displays excellent statistical data (Z' > 0.6). Moreover, this cell line has been validated with different stimuli and known inhibitors (shows correct EC50 and IC50 values) and serves as robust cellular readout for mTORC1 signaling.

PDCD4 Antibody, ABfinity™ Rabbit Oligoclonal (Invitrogen™)

PDCD4 Oligoclonal Antibody for Western Blot, IF, ICC

PDCD4 Antibody (1H15L6), ABfinity™ Rabbit Monoclonal (Invitrogen™)

PDCD4 Monoclonal Antibody for Western Blot, ICC, Flow

PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for Western Blot, IHC (P)

LanthaScreen™ Tb-anti-PDCD4 [pSer457] Antibody Kit

A part of the PI3K/AKT Pathway, PDCD4 (programmed cell death protein 4) is phosphorylated at Ser457 indirectly via mTORC1 (through the ribosomal protein p70 S6 kinase).

For use in combination with the LanthaScreen® PDCD4 HEK293E cell line (K1593) to evaluate inhibitors in medium to high throughput screening applications..

PDCD4 Synthetic Peptide (Invitrogen™)

Human PDCD4 Synthetic Peptide for BLOCK, Ctrl

PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for Western Blot, IHC

Phospho-PDCD4 (Ser457) Polyclonal Antibody (Invitrogen™)

Phospho-PDCD4 (Ser457) Polyclonal Antibody for Western Blot, IHC (P)

PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for Western Blot, IHC (P), IP

PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for IF, ICC

Phospho-PDCD4 (Ser67) Polyclonal Antibody (Invitrogen™)

Phospho-PDCD4 (Ser67) Polyclonal Antibody for Western Blot

Phospho-PDCD4 (Ser457) Polyclonal Antibody (Invitrogen™)

Phospho-PDCD4 (Ser457) Polyclonal Antibody for Western Blot, IHC (P)

PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for Western Blot, IF, ICC

PDCD4 Polyclonal Antibody (Invitrogen™)

PDCD4 Polyclonal Antibody for Western Blot, ELISA