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PIK3CA/PIK3R3 (p110 alpha /p55 gamma) Recombinant Human Protein (Invitrogen™)

This PIK3CA/PIK3R3 (p110 alpha /p55 gamma) recombinant human protein (full length) was expressed in insect cells. PIK3CA/PIK3R3 lipid kinase (p110 alpha /p55 gamma) or Phosphatidylinositol 3-kinase (PIK3) phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which serves as second messenger in growth signaling pathways. PIK3CA comprises a 110 kDa catalytic subunit and a regulatory subunit of either 85, 55, or 50 kDa. PIK3CA plays an important role in the metabolic actions of insulin, and a mutation in the PIK3CA has been associated with insulin resistance and plays an important role in glucose homeostasis in vivo. PIK3CA also plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses. PIK3CA/PIK3R3 (p110 alpha /p55 gamma) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PIK3CA/PIK3R1 (p110 alpha/p85 alpha) Recombinant Human Protein

PIK3CA (p110 alpha) is known to be involved in the PI 3-kinase⁄AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. PIK3CA is coexpressed and copurified with the regulatory subunit PI3KR1.

PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) Recombinant Human Protein (Invitrogen™)

This PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein (full length) was expressed in insect cells. PIK3CA (E545K)/PIK3R1 lipid kinase (p110 alpha E545K/p85 alpha) consists of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The PIK3CA catalytic subunit is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of PIK3CA is essential for vascular development and inactivation of PIK3Ca leads to severe defects in angiogenic sprouting and vascular remodeling. PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) Recombinant Human Protein (Invitrogen™)

This PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) recombinant human protein (full length) was expressed in insect cells. PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) lipid kinase is comprised of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. PIK3CA is frequently mutated or amplified in a variety of cancers including ovarian and colon and this kinase is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of PIK3CA is essential for vascular development and inactivation of PIK3CA leads to severe defects in angiogenic sprouting and vascular remodeling. PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PIK3CA Monoclonal Antibody (4F3) (Invitrogen™)

PIK3CA Monoclonal Antibody for Western Blot, IF, ICC, Flow

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for IHC (P)

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for IHC, pep-ELISA

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for Western Blot, IF, ICC, IHC (P)

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for Western Blot, IHC (P)

PIK3CA Monoclonal Antibody (H.843.0) (Invitrogen™)

PIK3CA Monoclonal Antibody for Western Blot, IHC (P), IP

PIK3CA Monoclonal Antibody (OTI6D1), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for Western Blot

PIK3CA Monoclonal Antibody (OTI6D9), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Monoclonal Antibody (OTI7A7), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Monoclonal Antibody (OTI8D6), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for Western Blot, IHC

PIK3CA Monoclonal Antibody (OTI7A7), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for Western Blot, IF, IHC

PIK3CA Monoclonal Antibody (OTI8D6), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Recombinant Rabbit Monoclonal Antibody (SJ0186) (Invitrogen™)

PIK3CA Recombinant Monoclonal Antibody for Western Blot, IF, ICC

PIK3CA Polyclonal Antibody (Invitrogen™)

PIK3CA Polyclonal Antibody for Western Blot

PIK3CA Monoclonal Antibody (OTI6D9), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

PIK3CA Monoclonal Antibody (OTI6D1), TrueMAB™ (OriGene)

PIK3CA Monoclonal Antibody for IHC (P)

CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line

The CellSensor® T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression (DBE-bla), along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs. Addition of doxycycline to these cells allows for FOXO3 transcription factor expression and subsequent beta-lactamase expression, which in turn can be down-regulated by insulin-mediated activation of the PI3K/AKT signaling pathway which leads to phosphorylation and inactivation of FOXO3 and concomitant suppression of beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of assay parameters, including: cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of insulin to antagonize doxycycline- induced FOXO3 activity (Figure 1). Additional testing data using RNAi and alternate stimuli are also available.

WORTMANNIN (Invitrogen™)

Wortmannin, a fungal metabolite, is a potent and selective inhibitor of phosphatidylinositol 3-kinase.

Format/Formulation: Lyophilized

TaqMan™ Mutation Detection Assay (castPCR) (Applied Biosystems™)

TaqMan Mutation Detection Assays are powered by competitive allele-specific TaqMan PCR (castPCR) technology to detect and measure somatic mutations in genes associated with cancer research. castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh-frozen tissue samples.

castPCR technology
Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.

Somatic mutation detection
By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays provide:

Higher specificity—designed to block the wild-type and amplify only the mutant
Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
Faster workflow—3 hours from sample to result

With 819 assays for 47 known cancer research genes available today like KRAS, BRAF, KIT, JAK2, the TaqMan Mutation Detection Assays provide an innovative tool for your cancer research.

All TaqMan Mutation Detection Assays have undergone extensive testing to ensure verify high sensitivity and specificity.