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CellSensor™ NFAT-bla Jurkat Cell Line

The CellSensor® NFAT-bla Jurkat cell line contains a beta-lactamase reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into Jurkat cells. This cell line can be used as a parental cell line to build specific G-protein coupled receptor (GPCR) assays after transfection of additional genes of interest. NFAT-bla Jurkat cells have been shown to be responsive to phorbol 12-myristate 13-acetate (PMA) and Ionomycin stimulation. Additional testing data using alternate stimuli is available upon request. Academic and non-profit customers, please inquire for special pricing.

PKC alpha Monoclonal Antibody (MC5) (Invitrogen™)

PKC alpha Monoclonal Antibody for Western Blot, IF, ICC, IHC (P), IP

PRKCA (PKC alpha) Recombinant Human Protein (Invitrogen™)

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

Phospho-PKC alpha (Thr638) Polyclonal Antibody (Invitrogen™)

Phospho-PKC alpha (Thr638) Polyclonal Antibody for Western Blot, IF, ICC

CellSensor™ NFAT bla HEK 293T Cell Line

The CellSensor® NFAT-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at fixed concentration of PMA. The CellSensor® NFAT-bla HEK 293T cell line responds to agonist treatment as expected from the literature and can be adapted for high-throughput screening (including coupling of receptors to the NFAT pathway) for agonists or antagonists with compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

LanthaScreen™ Tb-pSer (PKC) Antibody Kit

A general assay for the Protein Kinase C (PKC) family, the antibody detects the phosphorylation of the serine within the PKC substrate.

For use in combination with the Fluorescein-PKC substrate (PV3506) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein-PKC Substrate

A fluorescein-labeled peptide substrate for the Protein Kinase C (PKC) family.

For use in combination with the LanthaScreen® Tb-pSer (PKC Substrate) antibody (PV3560) to evaluate inhibitors in medium to high throughput screening applications.

Phospho-PKC alpha (Tyr657) Polyclonal Antibody (Invitrogen™)

Phospho-PKC alpha (Tyr657) Polyclonal Antibody for Western Blot

PKC alpha Polyclonal Antibody (Invitrogen™)

PKC alpha Polyclonal Antibody for Western Blot, IF, ICC, IHC (P), Flow

PKC Pan Polyclonal Antibody (Invitrogen™)

PKC Pan Polyclonal Antibody for Western Blot, IHC (P)

PKC alpha Polyclonal Antibody (Invitrogen™)

PKC alpha Polyclonal Antibody for Western Blot, IF, ICC, Flow, IP

Phospho-PKC alpha/beta-2 (Thr638, Thr641) Polyclonal Antibody (Invitrogen™)

Phospho-PKC alpha/beta-2 (Thr638, Thr641) Polyclonal Antibody for Western Blot, IP

PKC alpha Polyclonal Antibody (Invitrogen™)

PKC alpha Polyclonal Antibody for Western Blot, IHC (P)

Phospho-PKA alpha/beta (Thr197) Polyclonal Antibody (Invitrogen™)

Phospho-PKA alpha/beta (Thr197) Polyclonal Antibody for Western Blot

PKC alpha Polyclonal Antibody (Invitrogen™)

PKC alpha Polyclonal Antibody for Western Blot