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Pierce™ Anti-DYKDDDDK Magnetic Agarose (Thermo Scientific™)

Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as "FLAG", is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.

For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged proteins with elution buffer. The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purifications and screening of purification conditions.

Features include:
Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
High purity—optimized bind-wash-elute protocol enables high purity
High yield—special antibody conjugation method enables high yield
Rapid—entire purification protocol typically takes less than 40 mins
Economical—purification protocol allows multiple reuses
Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)

Characteristics of Pierce Anti-DYKDDDDK Magnetic Agarose:

Composition: anti-DYKDDDDK antibody covalently attached to a magnetic, highly crosslinked agarose support
Magnetization: ferrimagnetic with low remanence
Bead size: 10–40 µm
Bead concentration: 25% slurry in phosphate buffered saline, 0.01% Tween-20 detergent, 0.02% sodium azide, pH 7.2
Binding capacity: ≥3.2 mg DYKDDDDK-tGFP-His protein (~32 kDa)/mL settled beads

Pierce™ Ni-NTA Magnetic Agarose Beads (Thermo Scientific™)

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins. The beads are incubated with cell lysate containing His-tagged protein and then magnetically separated from the supernatant manually or through automation using an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Nonspecifically bound protein can be washed away before dissociating bound His-tagged protein with elution buffer. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.

Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >
Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >

Pierce Ni-NTA Magnetic Agarose Beads consist of highly crosslinked agarose beads embedded with magnetite and a covalently attached tetradentate nitrilotriacetic acid (NTA) chelator charged with divalent nickel ions. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added feature of magnetic handling. Magnetic agarose beads are a valuable tool for small-scale (~1 mg) purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications with agarose chromatography supports.

Features of Ni-NTA Magnetic Agarose Beads:
• High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
• High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
• Low non-specific binding—optimized purification protocol results in better tagged-protein purification
• Versatile—purify proteins using native or denaturing conditions
• Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

The high-performance, magnetite-containing, superparamagnetic magnetic agarose resin is validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.

Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.

Pierce™ Protein L Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein L Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification using manual or robotic magnetic separators.

Features of Protein L Magnetic Beads:

Selective—immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Low non-specific binding—stable, pre-blocked beads provide clean purification of antibody
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains. The beads are commonly used to purify monoclonal antibodies in cell culture supernatants supplemented with bovine serum as Protein L does not bind bovine IgG. The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.

Applications:
• Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads
• Purification of monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L IP and co-IP assays
• Purification of ScFv and Fab fragments containing kappa light chains

Thermo Scientific Pierce Protein L Magnetic Beads are typically used for purifying mouse and human antibodies containing kappa light chains from serum, cell culture supernatant or ascites. Protein L can bind a broader range of Ig classes than Protein A or Protein G, including IgG, IgM, IgA, IgE and IgD. Protein L binds strongly to human (kappa I, III and IV only), mouse (kappa I only), rat and pig immunoglobulins. It binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep immunoglobulins. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. The protocol for the Pierce Protein L beads has been optimized to allow for high recovery and high purity of isolated antibodies.

Pierce™ Magnetic ChIP Kit (Thermo Scientific™)

The Thermo Scientific Pierce Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR.

Features of the Magnetic ChIP Kit:

Rapid—obtain purified DNA that is ready for quantitative PCR in about 8 hours
Efficient and reproducible—micrococcal nuclease digestion and nuclear lysis are highly optimized
Sensitive—obtain results with as few as 10,000 cells (1 x 10^4)
Low background—Pierce Protein A/G Magnetic Beads are blocked in a non-DNA-containing reagent to minimize background
Complete—optimized positive control reagents are included: RNA polymerase II antibody and GAPDH promoter PCR primers

The Pierce Magnetic ChIP Kit contains sufficient reagents to perform 30 chromatin immunoprecipitation (ChIP) assays with appropriate controls using an optimized protocol. The kit includes reagents for cell lysis, capture of protein-DNA complexes, reversal of crosslinking, and DNA isolation. The blocked Pierce Protein A/G Magnetic Beads provide high binding capacity, low non-specific background, and work with many antibody species. These beads can be used manually with a magnetic stand or with automated platforms, such as the Thermo Scientific KingFisher Instruments. ChIP-validated and quality-guaranteed antibodies are also available for use with the Pierce Magnetic ChIP Kit.

Includes:
Kit contains reagents for chromatin preparation, IP, DNA purification, and positive controls (antibody and primers)

Requires:
In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument

Applications:
• Determine sites of specific protein-DNA interactions on genomic DNA by PCR or ChIP-Seq
• Monitor the effects of histone modifications or chemical agents on DNA binding

A successful ChIP assay requires a number of critical steps (crosslinking, chromatin preparation, immunoprecipitation) prior to detecting the target genomic DNA. Individually, each step in the ChIP protocol can be time-consuming to optimize. The Thermo Scientific Pierce Magnetic ChIP Kit simplifies the ChIP process, enabling accurate and reproducible results.

Protein-DNA complexes are first crosslinked in vivo with formaldehyde. The kit contains reagents to lyse cells and extract and solubilize the crosslinked complexes. The complexes are then incubated with a specific antibody and isolated using Pierce Protein A/G Magnetic Beads. After reversing crosslinks and digesting protein, the resulting DNA fragments are purified and are then ready for standard or quantitative PCR.

More Product Data
Analysis of androgen-dependent and -independent regulation of transcriptional activity

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Pierce™ Anti-c-Myc Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-c-Myc Magnetic Beads:

Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation
Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The Pierce Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Specifications:
Product Details:
Pierce Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0) 50 mM NaOH, or SDS-PAGE sample buffer, the protocol has been optimized for each of these conditions. Anti-c-Myc antibody can be used to detect c-Myc tagged protein by Western blot analysis.

Pierce™ Protein A/G Magnetic Agarose Beads (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein A/G Magnetic Agarose Beads provide a fast, convenient method for purification of immunoglobulins from serum, cell culture supernatant, or ascites. For antibody purification, the beads are incubated with the antibody sample and then magnetically separated from the supernatant. Nonspecifically bound serum or host cell protein can be washed away before dissociating bound antibody with elution buffer. The beads are removed from the solution manually using a magnetic stand or by automation using an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.

Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >
Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >

Pierce Protein A/G Magnetic Agarose Beads contain a 50.5 kDa Protein A/G recombinant fusion protein that is covalently attached to a magnetite-embedded agarose core particle. These beads are not simply a mixed immobilization of separate Protein A and Protein G polypeptides, nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads. The recombinant chimeric Protein A/G combines four IgG binding domains from Protein A and two binding domains from Protein G, making it a more general and convenient tool for purifying immunoglobulins.

Features of Protein A/G Magnetic Agarose beads:
• Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables IgG purification from nearly any mammalian species
• High capacity—sufficient for both routine and demanding purification procedures
• Low non-specific binding—optimized purification protocol results in better IgG purification
• Flexibility—convenience of IgG binding domains of both Protein A and Protein G on one bead
• Compatibility— beads are compatible with manual and automated applications
• Inert and stable—superior manufacturing method immobilizes Protein A/G by leach-resistant covalent bonds

The high-performance, magnetite-containing, superparamagnetic magnetic agarose beads are validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.

Our recombinant Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM, or serum albumin. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM, and murine serum albumin. Individual subclasses of mouse monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.

Pierce™ Protein A Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

Pierce™ Protein G Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein G Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein G Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein G, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein G coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein G Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein G Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein G can bind to antibodies from many different species, including mouse, human, rabbit, cow, goat and sheep. The protocol for the Pierce Protein G beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein G magnetic beads from other suppliers.

Pierce™ Anti-HA Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-HA Magnetic Beads:

Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation
Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Specifications:
Product Details:
Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.

Pierce™ Glutathione Magnetic Agarose Beads (Thermo Scientific™)

Thermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate. Pierce™ Glutathione Magnetic Agarose Beads are well suited for purifying GST fusion proteins from a soluble protein extract and are ideal for the purification of proteins expressed at low levels from diluted supernatants. The beads can be used in manual applications with a magnetic stand or automated applications with an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.

Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >
Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >

Features of Glutathione Magnetic Agarose Beads:
• High-performance beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS applications
• Stable affinity ligand—glutathione is covalently immobilized to particles, enabling leach-resistance and clean purification products
• High capacity—binding capacity is sufficient for both routine and demanding purification procedures

The high-performance, magnetite, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher™ 96 and KingFisher™ Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.

Glutathione S-transferase (GST) is a protein that is commonly added to the end of recombinant proteins to aid in their purification or detection. To make an affinity support, glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding. Addition of reduced glutathione in a physiologic buffer is sufficient to competitively displace and recover the GST fusion protein from the immobilized glutathione.

HisPur™ Ni-NTA Magnetic Beads (Thermo Scientific™)

Thermo Scientific HisPur Ni-NTA Magnetic Beads are high-capacity nickel-IMAC beads for affinity purification of His-tagged fusion proteins in manual or automated formats.

Features of HisPur Ni-NTA Magnetic Beads:

High capacity—equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding—the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast—protocol is completed in 1 hour
Scalable—process microliter to milliliter sample volumes
Versatile—purify proteins using native or denaturing conditions
Reagent compatible—can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats—protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni-NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput needs.

HisPur Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using B-PER Bacterial Protein Extraction Reagents. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the HisPur Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.

Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A/G Magnetic Beads:

High capacity—nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment
Low non-specific binding—stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix)
Flexibility—convenience of IgG binding domains of both Protein A and Protein G on one bead
Compatibility—beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments)
Assay consistency—magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes

These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G. This enables capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample. These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:
• IP and Co-IP experiments (see complete kit)
• Immunoprecipitation for analysis in non-reducing conditions
• Antibody purification

The recombinant Protein A/G that is immobilized onto the Pierce Magnetic Beads is a fusion of the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a convenient tool for investigating and purifying immunoglobulins. Thus, Pierce Magnetic Particles are not simply a mixed immobilization of separate Protein A and Protein G polypeptides, nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads.

Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™)

The Thermo Scientific Pierce Magnetic HA-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

Features of the HA-Tag Magnetic IP/Co-IP Kit:

Specific magnetic beads—covalently immobilized high-quality anti-HA monoclonal antibody enables high yields of immunoprecipitation products
Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding
Trouble-free elution—low-pH elution buffer ensures recovery of HA-tagged protein interaction complexes without antibody leaching contamination
Convenient and fast—complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hour
Versatile—magnetic beads are compatible with manual and automated magnetic separation workflows (e.g., Thermo Scientific KingFisher Instruments)

Unlike traditional IP procedures based on capture with Protein A/G beads, this kit uses magnetic beads containing pre-immobilized anti-HA antibody. These Pierce Anti-HA Magnetic Beads ensure specific binding of HA-tagged protein complexes from biological samples. Because the antibody is covalently attached to the beads, IP-targets are easily eluted and recovered without antibody contamination. The complete IP kit includes the magnetic beads, lysis/wash buffer, low-pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer for SDS-PAGE. Protocols are provided for both manual and automated magnetic separation workflows. Sufficient components are provided to perform 40 IP or co-IP assays.

The hemagglutinin (HA) peptide (YPYDVPDYA), derived from the human influenza virus HA protein, is one of several fusion protein tags used for recombinant protein expression. The Pierce Magnetic HA-Tag IP/Co-IP Kit uses a specific, high-affinity immobilized antibody (clone 2-2.2.14) for rapid purification of HA-tagged fusion proteins from bacterial and mammalian cell lysates, as well as from lysates prepared with the Pierce Human in vitro Translation Kits. The beads are incubated with a cell lysate containing HA-tagged protein, the fusion protein is captured, and the beads are subsequently washed and then eluted using low-pH elution buffer or non-reducing sample buffer. The protocol and buffers have been optimized for both IP and co-IP reactions, enriching for specific protein interaction complexes in the eluted samples. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.

For information about the binding capacity and other properties of the magnetic beads used in this kit, see Pierce Anti-HA Magnetic Beads (Part No. 88836).

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Pierce™ NHS-Activated Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce NHS-Activated Magnetic Beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments.

Features of NHS-Activated Magnetic Beads:

High capacity—at least four times higher binding capacity than NHS-activated magnetic beads from other suppliers
Easy to use—immobilize in a simple one-step reaction with minimal hands-on time
• Safe—no hazardous chemicals needed (e.g., sodium cyanoborohydride and cyanogen bromide)
Ligand-compatible—use to immobilize with nearly any primary amine-containing compound or affinity ligand
Low non-specific binding—the bead surface is pre-blocked and any nonreacted NHS-ester groups are fully quenched
Protocol-compatible—protein coupling to the beads and downstream applications can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)

The activated magnetic beads contain N-hydroxy-succinimide (NHS) functional groups that react with primary amines forming stable amide linkages. Once they are covalently attached, the immobilized proteins are highly resistant to leaching from the bead surface. When prepared beads are used in experiments, nonspecific binding is negligible because nonreacted NHS-ester groups are thoroughly blocked during the coupling procedure. Pierce NHS-Activated Magnetic beads can be coupled and processed either manually with a magnetic stand or with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:
• Immobilization of ligands for the purification of recombinant proteins
• Immobilization of antibodies for immunoprecipitations and co-immunoprecipitations free of antibody contamination in the eluates
• Immobilization of secondary antibody for the affinity purification of antibody subtypes from ascites, serum and cell culture supernatant

Pierce NHS-Activated Magnetic Beads offer a convenient way to conjugate any desired protein to a magnetic bead surface. The process does not require hazardous chemicals or lengthy reaction schemes. The beads are first incubated with protein for 1 to 2 hours in an amine free buffer at pH 7 to 9 to allow for covalent coupling through NHS-ester chemistry. The beads are subsequently washed and then any remaining active NHS-ester groups are quenched. The amine reactive chemistry and subsequent quench are completed in 3 to 4 hours. Coupled protein does not leach from the bead surface. In addition, the beads exhibit very low non-specific binding due to effective quenching and a proprietary blocking agent that coats the surface of the base particle. Following conjugation the prepared magnetic beads are typically used in affinity purification procedures.

Pierce™ ChIP-grade Protein A/G Magnetic Beads (Thermo Scientific™)

Magnetite- and protein-coated polymer beads at 10 mg/mL in water with sodium azide. Sufficient For: Use in approx. 250 typical ChIP assays

More Product Data
Analysis of androgen-dependent and -independent regulation of transcriptional activity

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