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Sensititre Electronic Pipette Product Overview Product Literature

Fit for Purpose ClipTip Pipette Tips Product Line Certificate Product Literature

Fit for Purpose ART Pipette Tips Product Line Certificate Product Literature

Fit for Purpose Finntip Pipette Tips Product Line Certificate Product Literature

Product Brochure: Nunc Serological Pipettes Product Literature

Product Sheet: Genexus Pipette Tips Manual / Product Insert

  • Pub. No.: 72517069a822ad6f0fee5b3c829ad58dcda132c8
  • Version: A.0
Catalog # A40266

Product Brochure: F1-ClipTip Pipetting System Brochure Product Literature

Product Brochure: Finnpipette Finntip Good Laboratory Pipetting Guide Product Literature

Product Brochure: Finnpipette Digital Pipettes: Classic style for timeless dependability and durability Product Literature

What are the differences in the protocols between the different versions of ExoSAP-IT reagent? Product FAQ

Answer

All versions of ExoSAP-IT reagent follow a simple one-pipette step protocol where 2 µl ExoSAP-IT reagent is added to 5 µl PCR product followed by incubation at 37°C for enzyme activation, then 80°C for inactivation. The incubation times differ as follows:
-ExoSAP-IT reagent (original formulation): 15 minutes at 37°C or 15 minutes at 80°C
-HT ExoSAP-IT Fast reagent: 7 minutes at 37°C or 7 minutes at 80°C
-ExoSAP-IT Express reagent: 4 minutes at 37°C or 1 minute at 80° C

Answer Id: E14405

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What are the differences between the ExoSAP-IT PCR cleanup reagents? Product FAQ

Answer

All of our ExoSAP-IT reagents have a similar function for PCR product cleanup, but differ in the areas listed below:

ExoSAP-IT Express PCR Product Cleanup
• Protocol time: 5 minutes
• Format: Single tube*, 8-tube strips, 96-well plate*
• Throughput level: Low to high; Recommended for processing any sample size
• Platform: Single- or multi-channel pipette, automated liquid handling platforms
• Freezes at -20°C: No
• Stability: -20°C for up to 2 years

ExoSAP-IT PCR Proudct Cleanup (our original formulation)
• Protocol time: 30 minutes
• Format: Single tube
• Throughput level: Low to mid; Recommended for processing 1-96 samples at a time
• Platform: Single-channel pipette
• Freezes at -20°C: No
• Stability: -20°C for up to 2 years

HT ExoSAP-IT Fast High-Throughput PCR Product Cleanup (for automated liquid handlers)
• Protocol time: 14 minutes
• Format: Single tube, 8-tube strips, 96-well plate
• Throughput level: High; Recommended for processing ≥96 samples at a time
• Platform: Automated liquid handling platforms
• Freezes at -20°C: Yes
• Stability: -20°C for up to 2 years; once thawed, stable at 4°C for 1 month and RT for 2 days

*Optional tracking dye available (Cat. No. 75002)

Answer Id: E21101

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How robust is the Nunc UpCell surface? Product FAQ

Answer

The UpCell surface is stable when standard cell culture conditions are applied, but scratching of the surface, for example, with a pipette tip, should be avoided, as this can impair the temperature-responsive properties of the product.

Answer Id: E17502

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I used the mirVana qRT-PCR miRNA Detection Kit and got no products in the no-template control (NTC) reaction. What could have happened? Product FAQ

Answer

Here are possible causes and solutions:

- Product from amplification of PCR primer-dimer: we recommend routinely running a no-template control reaction for each mirVana qRT-PCR Primer Set in the experiment. In successful miRNA amplification reactions, a single ~90 bp product is synthesized, which results in detection of a single product by melt curve analysis on a real-time thermal cycler. Smaller products (end-point) or products with lower Tm (real-time) may be observed in some no-template control reactions; these result from primer-dimer formation. They are typically only detectable late in the cycling protocol (>30 cycles). (Product from primer-dimer can also be amplified in no-RT control reactions.)
- Reagent contamination with PCR product: if a product of ~90 bp is observed in the no-template control reaction, there is a good chance that one or more of the PCR reagents, pipettors, or benchtops has been contaminated with PCR products. Unfortunately the only way to remedy contaminated reagent(s) is to replace them.
To avoid PCR contamination, clean the lab bench and the pipettors routinely with a DNA decontamination reagent such as DNAZap solution (Cat. No. AM9890). Always use barrier tips to pipette PCR reagents, and store completed PCRs in a different location than the PCR reagents.

Answer Id: E9757

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The A450 values that I got for the standard curve in your ELISA kit are lower than the example values shown in the product manual. Why? Product FAQ

Answer

There are 2 main causes of poor ELISA standard curves. First, the recommended method for solubilizing the kit standard may not have been followed. The standard should be reconstituted according to the directions indicated on the label, using the standard diluent provided in the kit. No other diluent should be used. The vial should then be swirled or mixed gently and then allowed to sit for 10 minutes at room temperature to ensure complete solubilization. This concentrated standard solution should be used within 1 hour of reconstitution. Also, it should be mixed gently again before preparing the dilutions in standard diluent according to the instructions provided in the product manual. Leftover standard can usually be stored frozen in small aliquots, unless specified otherwise in the product manual.

The second common reason for poor standard curves is that the HRP conjugate was not diluted correctly. The 100X HRP conjugate solution contains 50% glycerol, which makes it very viscous and difficult to pipet accurately. Here is what we suggest to solve this problem: First, let the vial of HRP conjugate come to room temperature. Then, stir it gently with a clean pipet tip to make sure that it is homogeneous. Use only the separate HRP conjugate diluent provided in the kit to dilute it, and follow the dilution instructions provided in the manual.

The key to diluting the HRP conjugate is to make sure that it is pipetted correctly. You should test that your pipettor accurately aspirates and dispenses the volume of the conjugate-glycerol mixture that is required. If possible, this pipettor should be calibrated so it is accurate and reliable. When you aspirate the viscous conjugate solution, it may take 5-10 seconds for the desired amount to enter the pipet tip. Before transferring the conjugate to the appropriate HRP diluent, make sure that the outside of the pipet tip is dry by wiping it with a lab tissue (e.g., Kimwipes tissue), taking care to ensure that the contents inside the tip do not get absorbed by the tissue. Pipet the conjugate into the diluent, and then rinse out the tip by pipetting up and down several times. It is important to get every last bit of conjugate out of the tip. Next, seal the container and mix it gently but thoroughly by rocking it or turning it upside down. This is crucial because the glycerol carries the conjugate quickly down to the bottom of the tube. If the diluted conjugate is not mixed adequately, the concentration of the HRP conjugate will not be what is required.

Once the HRP conjugate is diluted and mixed gently but well, use it within 15 minutes. Remember that the HRP conjugate diluent is the only acceptable diluent for the HRP conjugate. The diluted HRP conjugate should not be saved because the HRP activity is labile, and it should never be stored and reused.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Answer Id: E12628

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What is the tracking dye in ExoSAP-IT Express with Tracking Dye? Product FAQ

Answer

It is an inert blue color dye for easy tracking of ExoSAP-IT Express reagent as it is pipetted into wells for PCR cleanup.

Answer Id: E14417

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