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A light stabilizer (Tinuvin 770) that elutes from polypropylene plastic tubes is a potent L-type Ca(2+)-channel blocker. Citations & References

  • Authors: Glossmann H, Hering S, Savchenko A, Berger W, Friedrich K, Garcia ML, Goetz MA, Liesch JM, Zink DL, Kaczorowski GJ
  • Journal: Proc Natl Acad Sci U S A
  • PubMed ID: 8415734
Catalog # D7441(Discontinued)

I am using the Pierce Direct Magnetic IP/Co-IP Kit. The magnetic beads formed an aggregate during the coupling process; how do I avoid this? Product FAQ

Answer

The proteins on the bead surface may adhere to the plastic tube and to each other. To avoid this, after the quenching step, add a mild surfactant at a final concentration of 0.05% (v/v) ( e.g., Tween-20 Detergent, Cat. No 85113) to the washes

Note: Do not use detergent in the coupling step.

Answer Id:: E13054

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I am interested in performing Luminex assays using BioSource kits, and I have a Luminex xMAP system. Besides the kits and system, what other reagents and equipment will I need? Product FAQ

Answer

The following is a list of general lab supplies that are required for running BioSource immunoassays on the Luminex xMAP system:
1) Vacuum manifold
2) Sonicating water bath
3) Orbital shaker
4) Vortexer
5) Repeating and/or multi-channel pipetter (not required, but recommended)
6) Calibrated adjustable precision pipettes, with disposable plastic tips
7) Glass/plastic tubes and racks for preparing reagents
8) Graduated cylinder and container for preparing wash solution
9) Aluminum foil
10) Deionized or distilled water.

Answer Id:: E5180

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Media for Automation (MFA) Tubes [EN] Product Literature

Lot # 7269040010 Certificate

Catalog # DS3111-0025

Lot # 7241018010-COC Certificate

Catalog # DS3111-0029

Lot # 7241027010-COC Certificate

Catalog # DS3111-0017

Lot # 7209667010-COC Certificate

Catalog # DS3111-0016

Lot # 7118928020-COC Certificate

Catalog # DS3111-0018

Lot # 7197220010-COC Certificate

Catalog # DS3111-0030

Lot # 660251-COC Certificate

Catalog # DS3111-0018

Lot # 7123020060-COC Certificate

Catalog # DS3111-0018

Lot # 7188338010 Certificate

Catalog # DS3111-0018

Which plastic consumables should I use on my real-time PCR (qPCR)/thermal cycler (PCR) instrument? Product FAQ

Answer

Please select your real-time PCR (qPCR) or thermal cycler (PCR) instrument model from the drop-down menu in the qPCR and PCR Plastics Selection Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html) to see the list of all validated plastic consumables (plates, tube strips, single tubes, caps, seals, adhesive films, and accessories) we offer that are compatible with your instrument.

Answer Id:: E18748

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What types of plastics can I use with the ProFlex PCR System? Product FAQ

Answer

The plates and tubes will be specific to the block type. Please refer to our PCR Tubes, Plates & Accessories Plastics Selection Guide (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.

Answer Id:: E7356

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