SDS

Pluronic® F-68 10% (100X)

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Product FAQ

What is Pluronic F-68 Non-ionic Surfactant (100X) dissolved in?

Answer

It is a 10% solution of Pluronic F-68 in distilled water.

Answer Id: E17429

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Product FAQ

Do I need to remove Anti-Clumping Agent and Pluronic F-68 from the medium while growing Expi293 cells? How about during transfection of these cells?

Answer

You can have Anti-Clumping Agent and Pluronic F-68 in the medium while growing Expi293 cells. You need to remove Anti-Clumping Agent at least 2 passages prior to transfection of Expi293 cells because it interferes with transfection. You can add it back to the medium after transfection. Pluoronic F-68 can be present during transfection as it does not interfere with transfection.

Answer Id: E17481

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Citations & References

The fate of Pluronic F-68 in chondrocytes and CHO cells.

  • Authors: Gigout A, Buschmann MD, Jolicoeur M,
  • Journal: Biotechnol Bioeng (2008) 100:975-987
  • PubMed ID: 18393312
Catalog #

Product FAQ

I am purifying my protein expressed in insect cells by ammonium acetate precipitation and unable to get a precipitate. What could be the problem?

Answer

If you were using SF-900 II SFM, that is incompatible with the ammonium acetate precipitation method. Our SF-900 II Serum-Free Medium contains the block co-polymer non-ionic surfactant Pluronic F-68, which has been found to interfere with ammonium acetate precipitation. If you are putting the un-concentrated supernatant over the column, this should not cause a problem. If the supernatant has been concentrated, the Pluronic acid will need to be removed using a column.

Answer Id: E9457

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Product FAQ

Why are shear forces a consideration in culturing my insect cells?

Answer

Suspension culture techniques generate mechanical shear forces. Factors that contribute to the total shear stresses experienced by cells in suspension culture include the size and type of impellers within stirred vessels, the size and velocity of bubbles in airlift or sparged bioreactors, and the resulting turbulent action at the culture surface.

During suspension cell culture, most insect cell lines require shear force protection. Although serum concentrations between 5% and 20% in medium appear to provide some protection from shear forces, we recommend that all suspension cultures, whether serum-free or serum-supplemented, be supplemented with a shear force protectant such as Pluronic F-68 (Most commercially available media will contain Pluronic F-68).

Answer Id: E3046

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Product FAQ

Does FreeStyle 293 Expression Medium contain any components that would interfere with concentrating secreted proteins from HEK 293 cells via ammonium sulfate precipitation?

Answer

Yes, the FreeStyle 293 Expression Medium contains Pluronic F-68 and lipids that would interfere with ammonium sulfate precipitation. Dialysis or tangential flow filtration can be used to remove lipids and Pluronic F-68 from the medium. Then you can concentrate the medium with ammonium sulfate.

Answer Id: E17392

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Product FAQ

Which buffers are compatible with the Qdot Streptavidin Conjugates?

Answer

The Qdot 605 Streptavidin conjugates have stable emission in a number of buffers, across a range of pH. At working concentrations, the quantum yield and colloidal dispersion of these materials have been found to be remarkably stable across pH 6-9 (not investigated outside this range) in Tris, HEPES, phosphate, and borate buffers. The Qdot 605 Streptavidin conjugate is stable and non-aggregated in buffered NaCl up to 200 mM at working concentrations. Higher salt concentrations may result in microscopic precipitation, but do not appear to cause bulk precipitation of the materials at working dilutions. In addition, a number of surfactants and additives such as Tween 20, Triton X-100, Pluronic F-68, NDSB-201, and EDTA, among others have been shown to maintain the fluorescence in 0.05% concentrations. In contrast, gelatin and dextran sulfate were both found to promote aggregation of the Qdot 605 Streptavidin conjugate at 0.05% concentrations, and should be avoided in labeling applications. In general, we recommend storage of the Qdot nanoparticle conjugate at the concentration at which it was shipped, rather than at a higher dilution. Storing materials at working dilution over longer periods of time may result in substantial performance loss. While we have not characterized the stability of the other Qdot conjugates in this variety of buffers, we anticipate similar levels of stability.

Answer Id: E12230

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Manual / Product Insert

Manual:User Guide: OpenArray AccuFill System(English)

Version: 31 JUL 2011

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