Documents & Support

The central European or UK power cord for the Power Blotter System/Power Blotter XL System does not work in Switzerland. Do you offer a Swiss power cord? Product FAQ

Answer

Yes, you may purchase the Universal Cable Kit (Cat. No. 4377117).

Answer Id:: E17217

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Can I use the Power Blotter System/Power Blotter XL System with my own homemade transfer buffer? Product FAQ

Answer

Yes, the Power Blotter System/Power Blotter XL System is an open system and you can use your own transfer buffer. However, keep in mind that your own transfer buffer will result in a prolonged transfer time (˜60 min), whereas a fast transfer (5-10 min) can be achieved using the Power Blotter 1-Step Transfer Buffer (5X) or the Pierce 1-Step Transfer Buffer.

Answer Id:: E17207

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Is it possible to do gel staining with the Power Blotter System/Power Blotter XL System? Product FAQ

Answer

Even though the Power Blotter System/Power Blotter XL System does not have the staining program, this can be introduced. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.

Answer Id:: E17219

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Does the Power Blotter System/Power Blotter XL System allow fast transfer? Product FAQ

Answer

Yes, utilization of the Power Blotter 1-Step Transfer Buffer (5X) allows fast transfer (within 5 min for low MW proteins/within 7 min for mixed range MW proteins/within 10 min for high MW proteins). The Power Blotter can also be used with the Pierce 1-Step Transfer Buffer (Cat. No. 84731, 84731X5, or 84742) for fast transfer.

Answer Id:: E17208

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What is the difference between the Power Blotter System and the Power Blotter XL System? Product FAQ

Answer

The Power Blotter System (Cat. No. PB0012) comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter Cassette (Cat. No. PB0002). The Power Blotter System can transfer one midi or up to two mini gels at the same time. The Power Blotter XL System (Cat. No. PB0013) on the other hand comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter XL Cassette (Cat. No. PB0003). The Power Blotter XL System can transfer up to two midi or four mini gels at the same time.

Answer Id:: E17220

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My PowerEase 300W Power Supply stopped working with an alarm and the screen displays "Leakage". Can you please help? Product FAQ

Answer

This is most likely due to a ground leak detected during the run. Check the electrophoresis system for improper grounding and restart the power supply by turning the Power switch off and on.

Answer Id:: E11010

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Can I use PVDF and nitrocellulose membranes for transfer of gels using the Power Blotter System/Power Blotter XL System? Product FAQ

Answer

Yes, both PVDF and nitrocellulose membranes are compatible with the Power Blotter System/Power Blotter XL System. You can use 0.2 µm and 0.45 µm pore size membranes. Our Power Blotter Pre-cut Membranes and Filters (PVDF and nitrocellulose) come with 0.45 µm pore size membranes. Our Power Blotter Select Transfer Stacks (PVDF and nitrocellulose) come with 0.2 µm pore size membranes.

Answer Id:: E17214

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What is the power consumption of the Thermal Printer for iBright Imaging Systems? Product FAQ

Answer

In stand-by mode, the power consumption of the Thermal Printer for iBright Imaging Systems is 10 Watts, and during the printing process, it is 115 Watts.

Answer Id:: E18212

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My ZOOM Dual Power Supply stopped running with an alarm and the screen displays shows "LEAKAGE". What is the problem? Product FAQ

Answer

This indicates that a ground leak was detected during the run. Check the electrophoresis system for improper grounding. Restart the power supply by turning the Power switch off and on.

Answer Id:: E11054

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I am getting a "Change in Load" Error on the PowerEase 90W Power Supply? Why is this? Product FAQ

Answer

Here are likely reasons why this might happen:

- Electrophoresis systems were added or removed during a run
- Buffer leaking in a connected system
- Excessive temperature increase
- Excessive buffer evaporation
- Loose connection in a connected system
- Current set too low

Answer Id:: E11023

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What are the power supply considerations for the ZOOM IPGRunner system? Product FAQ

Answer

The ZOOM IPGRunner System should be used with an external DC power supply designed for IEF and electrophoresis applications. This power supply must:

*Be isolated from the ground so that the DC output is floating.
*Be programmable, with a 4-protocol minimum.
*Be able to operate at low current (<1 mA) as IEF is performed at very low current.
Note: Many power supplies automatically shut off when the current drops below 1 mA. You will need a power supply capable of overriding the low current shut-off feature.

The electrical leads of the ZOOM IPGRunner Lid are recessed and may not fit into some power supply units. To allow connection of the ZOOM IPGRunner power leads with certain power supplies, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).

Answer Id:: E10686

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I want to prepare my own transfer stack for use with the Power Blotter System/Power Blotter XL System. Can I use any filter paper? Product FAQ

Answer

Yes, but you need to ensure that the total thickness of the filter paper stack on either side of the gel/membrane does not exceed 1.8 mm.

Answer Id:: E17209

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Can I transfer EMSA gels using the Power Blotter System/Power Blotter XL System? Product FAQ

Answer

Yes. You may use the same method that was used with the G2 Fast Blotter: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/transfer-emsa-gels-using-g2-fast-blotter.html

Answer Id:: E17212

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