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A comparison of fluorescamine and o-phthaldialdehyde as effective blocking reagents in protein sequence analyses by the Beckman sequencer. Citations & References

  • Authors: Bhown AS, Cornelius TW, Volanakis JE, Bennett JC
  • Journal: Anal Biochem
  • PubMed ID: 6614468
Catalog #
  • F20261(Discontinued)
  • F2332(Discontinued)
  • P2331MP(Discontinued)
  • F2345

Can I use samples treated with RNAlater reagent for protein analysis? Product FAQ

Answer

RNAlater reagent will denature proteins; therefore, protein obtained from samples stored in RNAlater reagent are suitable for applications such as Western blotting or gel electrophoresis, but not for applications that require native protein.

Answer Id:: E6649

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A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly. Citations & References

  • Authors: Ohn T, Kedersha N, Hickman T, Tisdale S, Anderson P,
  • Journal: Nat Cell Biol
  • PubMed ID: 18794846
Catalog #

Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification. Citations & References

  • Authors: Dias WB, Cheung WD, Wang Z, Hart GW,
  • Journal: J Biol Chem
  • PubMed ID: 19506079

Isotope-tagged crosslinking reagents. A new tool in mass spectrometric protein interaction analysis. Citations & References

  • Authors: Muller, D.R. et al.
  • Journal: Anal Chem
  • PubMed ID: 0

The cytoplasmic tail dileucine motif LL572 determines the glycosylation pattern of membrane-type 1 matrix metalloproteinase. Citations & References

  • Authors: Ludwig T, Theissen SM, Morton MJ, Caplan MJ,
  • Journal: J Biol Chem
  • PubMed ID: 18955496

A novel manual method for protein-sequence analysis. Citations & References

  • Authors: Chang JY, Creaser EH
  • Journal: Biochem J
  • PubMed ID: 822842
Catalog # D1520

What is the Lumio recognition sequence in the Expressway Lumio Expression and Detection System? Product FAQ

Answer

The Lumio recognition sequence is a small 6 amino acid sequence of Cys-Cys-Pro-Gly-Cys-Cys. The Lumio detection reagent binds this recognition sequence with high specificity and affinity, causing a bright fluorescent signal for real-time protein production analysis and immediate in-gel protein detection.

Answer Id:: E9637

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Can I use the No-Stain Protein Labeling Reagent with an orange or red dye (i.e., will the signal appear in channel 2, 3, 4 or 5, not just channel 1 in the iBright imager)? Product FAQ

Answer

Channels 3, 4 and 5 on the iBright imager can be used for multiplexing without interference while using the No-Stain Protein Labeling Reagent. Channels 1 and 2 on the iBright imager will show interference. Fluorophores for multiplexing that are compatible with the No-Stain Protein Labeling Reagent are listed here, under the “Secondary Abs” tab (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/total-protein-normalization.html).

Answer Id:: E17978

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What do antibody pair kits contain, how many samples can I process, and how can I find a list of antibody pair kits by target? Product FAQ

Answer

Antibody pair kits contain capture antibody, detection antibody, recombinant standard and HRP conjugate. Each contains enough reagents to process forty 96-well plates. A list of Antibody Pair Kits (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) is available by target.

Answer Id:: E12615

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What do Reagent Sets contain, how many samples can I process, and how can I check if a Reagent Set is available for my target? Product FAQ

Answer

Reagent Sets contain Capture Antibody, Detection Antibody, Recombinant Standard, HRP Conjugate, TMB Substrate and Stop Solution. Each contains enough reagents to process five 96-well plates. Reagent Sets are included in the main list of ELISA (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) kits (search by “Reagent Set”).

Answer Id:: E12614

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Following the release of the iBlot 2 Gel Transfer Device, are consumables for the original iBlot Gel Transfer Device still available? Product FAQ

Answer

Yes, all original iBlot stacks are still available for purchase. You can find them in the original iBlot Gel Transfer Device Reagents and Resources (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system/reagents-resources-original-iblot-gel-transfer-device.html). However, please note that they are not compatible with the iBlot 2 Gel transfer Device.

Answer Id:: E7039

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Brochure: Sanger sequencing—Generate high-quality data with our proven workflow Product Literature

I need more ProcartaPlex Streptavidin-PE for my assay. Can I purchase this item separately? Product FAQ

Answer

Yes, ProcartaPlex Streptavidin-PE (Cat. No. EPX-SAPE-000) is available as a stand-alone item. We offer most of our PorcartaPlex buffers and reagents as stand-alone items. A complete list of what is currently available can be found on our website here: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/luminex-multiplex-assays/procartaplex-immunoassays/procartaplex-supplemental-products.html.

Answer Id:: E16833

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What is the detection range for the BCA Protein Assay? Product FAQ

Answer

For the standard protocol, the detection range is 20-2,000 µg/ml. For the enhanced test tube protocol, the detection range is 5-250 µg/ml. The Micro BCA Protein Assay Reagent will detect 0.5-20 µg/ml of protein in the test tube assay and 1-20 µg/ml for the microplate assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Answer Id:: E8338

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