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What is the difference between the Pierce Bradford Protein Assay Kit and the Pierce Bradford Plus Protein Assay Kit? Product FAQ

Answer

Pierce Bradford Plus Protein Assay Kit has all the advantages of the regular Pierce Bradford Protein Assay plus the added benefit of having a more linear standard curve. The added linearity of the standard curve translates to higher accuracy in the protein assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E13424

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Which Thermo Scientific protein assay is the best or the most reliable? Product FAQ

Answer

The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Answer Id: E8341

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What BCA protein assay kits do you recommend for NanoDrop 2000/2000c Spectrophotometers (Cat. Nos. ND-2000, ND-2000C)? Product FAQ

Answer

NanoDrop 2000/2000c Spectrophotometers (Cat. Nos. ND-2000, ND-2000C) are compatible with most commercial BCA protein assay kits. We offer a number of BCA protein assay kits, including, but not limited to, the BCA Protein Assay Kit (Cat. No. J63283.QA), Micro BCA Protein Assay Kit (Cat. No. 23235, and Pierce BCA Protein Assay Kit (Cat. No 23225, 23227).

To identify, compare, and choose the best protein assay for your sample type and application, you can use our interactive Protein Assay Selection Guide.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E21948

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Do I need to make a standard curve each time I perform the BCA Protein assay? Product FAQ

Answer

For optimal accuracy, make a standard curve each time a protein assay is performed. Measure standards in duplicate for the test tube assay and in triplicate for the plate assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Answer Id: E8335

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Does the Qubit Protein Assay work well in the presence of detergents? Product FAQ

Answer

The Qubit Protein Assay is compatible with very small amounts of detergent. See “Contaminants Tolerated by the Qubit Protein Assay” Table 2 on page 6 of the Qubit Protein Assay Kit product manual (https://tools.thermofisher.com/content/sfs/manuals/Qubit_Protein_Assay_UG.pdf) for specific amounts.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E20737

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Are there any reagents/chemicals I should avoid in my protein assay sample buffer? Product FAQ

Answer

Each protein assay method has its particular sensitivities. Generally, the following substances should be avoided for the given protein assay method:

  • BCA and Micro BCA Assays: reducing agents and chelators, and strong acids and bases
  • BCA Reducing Agent Compatible Assay: chelators 
  • 660 nm Assay: ionic detergents
  • Pierce Bradford Protein Assay Kit and Pierce Bradford Plus Protein Assay Kit: detergents
  • Modified Lowry Assay: detergents, reducing agents, and chelators 


Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E15579

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Does Y-PER Yeast Protein Extraction Reagent (Cat. No. 78990) yield samples that are compatible with the Pierce BCA Protein Assay Kit (Cat. No. 23225)? Product FAQ

Answer

Yes, Y-PER Yeast Protein Extraction Reagent is compatible with our Pierce BCA Protein Assay Kit. Please see our Protein assay compatibility table in this Tech Tip (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E18964

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What proteins do you offer as standards for your protein assays? Product FAQ

Answer

We offer BSA and BGG as protein standards for protein assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E15554

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Which protein assays are dye-binding based chemistries? Product FAQ

Answer

Pierce Bradford Protein Assay Kit and Pierce Bradford Plus Protein Assay Kit are variations on the use of Coomassie G-250 dye as a colorimetric reagent for the detection and quantitation of total protein first reported by Bradford in 1976. The Thermo Scientific 660 nm Protein Assay is a dye-based reagent that offers the same convenience as Coomassie-based assays while overcoming several of their disadvantages. In particular, the 660 nm Assay is compatible with most detergents and produces a more linear response curve (the detailed assay chemistry is proprietary). Our fluorometric protein assays are also based on dye binding chemistries.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E15550

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Which protein assay is my lysis buffer compatible with? Product FAQ

Answer

Each method has its particular sensitivities. Apart from the information presented in the product-specific instruction booklet, we offer an expanded protein assay compatibility table in this Tech Tip.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E15575

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Is the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible, 0.125-10 mg/mL (included in the Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit and available separately as Cat. No. A56979) compatible with other protein assays? Product FAQ

Answer

The Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible, 0.125-10 mg/mL is currently only recommended for use with the Pierce Dilution-Free Rapid Gold BCA Protein Assay. We have not validated its use with other assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Answer Id: E22200

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I have a Quant-iT protein assay kit and would like to use it on the Qubit fluorometer. Can I do this? Product FAQ

Answer

Yes, the Quant-iT Protein Assay Kit (https://tools.thermofisher.com/content/sfs/manuals/Quant_iT_Protein_Assay_UG.pdf) has directions for performing this application.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E15568

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How does the Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible compare with the original Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236)? Product FAQ

Answer

The Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible (Cat. No. A55866) includes the same assay reagent as the Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236), formerly called the Pierce Coomassie Plus (Bradford) Assay Kit. The only difference between the two products is the BSA protein standard format. While the Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236) includes the Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209), the Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible (Cat. No. A55866) includes BSA protein standards that are pre-diluted and packed in a convenient, multichannel pipette-friendly format. This format provides the following advantages:

-No protein standard dilution step needed
-High-throughput (multichannel pipette compatible)
-Blank tube provided for background correction
-Reduced hands-on assay time
-Reduced chances for pipetting errors

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Answer Id: E22213

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What protein assays do you offer for total protein quantitation? Product FAQ

Answer

We offer several types of protein assays including the: BCA Assay, BCA-RAC (Reducing Agent Compatible) Assay, Micro BCA Assay, 660 nm Protein Assay, Pierce Bradford Plus Protein Assay Kit, Pierce Bradford Protein Assay Kit, Modified Lowry Assay, colorimetric and fluorometric Peptide Assays, CBQCA kit, EZQ kit, Quant-iT kits, NanoOrange, and the Qubit kits.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E15539

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How does the Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards compare with the original Pierce BCA Protein Assay Kit (Cat. No. 23225, 23227)? Product FAQ

Answer

The Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards (Cat. No. A55864, A55865) includes the same assay reagents (BCA Protein Assay Reagent A and BCA Protein Assay Reagent B) as the Pierce BCA Protein Assay Kit (Cat. No. 23225, 23227). The only difference between the two products is the BSA protein standard format. While the Pierce BCA Protein Assay Kit (Cat. No. 23225, 23227) includes the Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209), the Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards (Cat. No. A55864, A55865) includes BSA protein standards that are pre-diluted and packed in a convenient, multichannel pipette-friendly format. This format provides the following advantages:

-No protein standard dilution step needed
-High-throughput (multichannel pipette compatible)
-Blank tube provided for background correction
-Reduced hands-on assay time
-Reduced chances for pipetting errors

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Answer Id: E22210

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