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Accurately quantifying total protein concentration is a key step in most experiments and workflows involving isolation, separation, and analysis of proteins. We offer a variety of assay reagents, kits, and standards for protein quantitation by fluorescent or colorimetric detection with fluorometers,...

Most colorimetric protein assay methods can be divided into two groups based on the type of chemistry involved: those involving protein-copper chelation with secondary detection of the reduced copper and those based on protein-dye binding with direct detection of the color change associated with the...

Fluorescence-based protein quantification detection methods provide superior sensitivity which means you use less of your protein samples for quantitation and have more samples available for your experiment.

The Qubit Protein Assay Kits are designed to make protein quantification easy while providing accurate quantification. Qubit protein assays require only 10–15 minutes of room temperature incubation, eliminating long incubation periods or exposure to elevated temperatures.

Precast gels, running buffers, molecular weight markers, and gel stain for sample preparation, loading, and analysis when studying proteins by electrophoresis (SDS-PAGE), Kits, reagents, and devices, including iBlot 2 dry blotting system, SuperSignal chemiluminescent substrates, and iBind system for...

Protein concentration quantitation is an integral part of any laboratory workflow involving protein extraction, purification, labeling or analysis. Pierce Protein Assays provide a wide range of options for accurate protein concentration determination based on assay time, sensitivity, compatibility,...

Learn about methods and technologies for identifying and measuring proteins. Understand protein quantitation reagents, ELISA and other immunoassays, protein interaction and activity assays, enzyme reporter assays, protein microarrays, and immunoprecipitation and pull-down tools.

For Research Use Only. Not for use in diagnostic procedures., View all What protein assays do you offer for total protein quantitation? I need to measure the concentration of a peptide, which protein assay can I use? Why is the choice of protein standard important?

With most protein assays, sample protein concentrations are determined by comparing their assay responses to that of a dilution-series of standards whose concentrations are known. Protein samples and standards are processed in the same manner by mixing them with assay reagent and using a...

No one reagent can be considered to be the ideal or best protein assay method. Each method has its advantages and disadvantages. Identify, compare and choose the best protein assay using the protein assay interactive product selector.

We are dedicated to your success.Get back on track. View our expert recommendations for commonly encountered problem scenarios related to your protein assays and quantitation experiments. View the relevant questions below:, Browse our FAQ database for more information ›, Beginning your experiment?

For Research Use Only. Not for use in diagnostic procedures., View all Which antibodies have you tested in a TaqMan® Protein Assay? How do I choose an antibody for a TaqMan® Protein Assay? Do I have to use a biotinylated antibody? What if I can’t find one? How do I biotinylate my antibody?

Whether you are detecting or quantifying your protein using a colorimetric or fluorescent-based assay, find valuable support information ranging from choosing the right assay with background information about the assay s we offer, to setting up of the assay and help with troubleshooting the results.

Optimize your protein assays and quantitation experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Having problems with your...

TaqMan® Protein Assays are an adapted form of PLA™, a proximity ligation assay technology [1,2] that combines antibody-protein binding with detection of the reporter nucleic acid by real-time PCR. We have optimized this technique for use with crude cell and tissue lysates and employ TaqMan® Assays...