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Babu Antharavally, Ph.D.; Xiaoyue Jiang, Ph.D.1; Robert Cunningham, Ph.D.; Ryan Bomgarden, Ph.D.; Yi Zhang, Ph.D.1; Rosa Viner, Ph.D.1; John C. Rogers, Ph.D., Mass spectrometry (MS) has become a prominent technique in biological research for the identification, characterization, and quantification...

Learn about methods and technologies for peptide and protein analysis by high performance liquid chromatography and mass spectrometry (LC/MS). Understand sample prep tools, mass and isotope labeling strategies for quantitative proteomics, and the basics of MS data and analysis.

We offer a wide range of products for protein mass spectrometry. Our discovery quantitation reagents and kits enable higher multiplexing using SILAC NeuCode technology or TMT11plex isobaric labeling. Achieve relative or absolute quantitation for targeted applications by using SureQuant Targeted MS...

View all What kind of substances in my sample would interfere with mass spectrometry analysis? What is the best way to remove small contaminants from my sample for mass spectrometry analysis? Can I use protein desalting columns for cleanup of peptide samples?

Proper sample preparation is a critical step in the proteomics workflow because the quality and reproducibility of these steps significantly impact the separation and identification capabilities of mass spectrometers.

Introduction to protein sample preparation Lysate preparation Protein denaturation, reduction, alkylation and digestion Peptide enrichment and clean-up Recommended reading, SILAC Metabolic Labeling Systems SILAC Quantitation Workflow Modification Reagents for Proteins and Peptides MS and HPLC...

View all What is the difference between Relative and Absolute Quantitation? Which type of quantitation is used for proteomic samples? What is the main purpose of Tandem Mass Tag (TMT) reagents? What type of mass spectrometer can I use for Tandem Mass Tag (TMT) analysis?

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios., View the relevant questions below:, Browse our FAQ database for more information ›, Having problems with your experiment?

Sample preparation remains one of the largest bottlenecks associated with mass spectrometry (MS) analysis, and consistent and reproducible sample preparation can make the difference between a successful analysis and a failed outcome.

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios., View the relevant questions below:, Browse our FAQ database for more information ›, Having problems with your experiment?

Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs., View the relevant questions below:, Browse our FAQ database for more information ›, Having problems with your experiment?

Gas chromatography mass spectrometry (GC-MS) sample preparation is performed on smaller and more volatile samples including environmental pollutants, industrial byproducts, food contaminants, pesticides, and metabolites of illicit and designer drugs.

Thermo Scientific™ MS-grade crosslinkers are high-quality reagents that are available in multiple packaging options and sizes. We offer extensive technical expertise and support for various applications, as well as validation of these products in workflows using Thermo Scientific mass spectrometers.

For Research Use Only. Not for use in diagnostic procedures., View all What protein assays do you offer for total protein quantitation? I need to measure the concentration of a peptide, which protein assay can I use? Why is the choice of protein standard important?

All samples require some form of preparation prior to study or analysis by mass spectrometry to remove detergents, reduce the complexity of the sample when focusing on specific proteins and/or tag proteins for identification/quantitation.