Fluorescent western blotting is growing in popularity because it allows the ability to perform multiplex detection, where multiple proteins can be detected at the same time. Historically, the instrumentation available for fluorescent detection was not able to offer the sensitivity required by many...
Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications . Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes.
View all available protein ladders Find the right protein ladder for your application and gel system. Use the interactive tool below to see our recommended protein ladders migration in the different gel chemistries.
Chemiluminescent detection for western blotting is popular because it offers several advantages over other detection methods. Using chemiluminescence allows multiple exposures to be made, which enables optimization of signal to noise.
Reliable transfer of high molecular weight (HMW) proteins (i.e., >150 kDa) from a gel to membrane during western blotting is a common challenge. Many factors affect the efficiency at which HMW proteins transfer.
Thermo Scientific DyLight microscale protein labeling kits contain NHS ester-activated derivatives of our high-performance DyLight dyes designed specifically for labeling small quantities of antibodies or proteins.
Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs., View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
HABA is a dye with absorbance and avidin-binding properties that help enable the quantitation of biotin in a sample. When HABA is bound to avidin, the complex absorbs light at 500 nm (e = 34,000/M cm).
Explore: Protein electrophoresis products Download: Protein Electrophoresis Handbook, Protein electrophoresis is a standard laboratory technique by which charged protein molecules are transported through a solvent by an electrical field.
Get the latest information on the analysis of biotherapeutic proteins such as monoclonal antibodies (mAbs) in their intact form. UHPLC is ideal for identifying modifications such as charge variants, as well as higher order structures through aggregation.
Here is a short description of markers and reagents that are more commonly used for DNA analysis. DNA molecular weight markers Ambion offers basic DNA molecular weight markers for routine analysis of end point PCR, restriction digests, or other analyses on native agarose gels.
FGF, also known as fibroblast growth factor, represents a family of approximately two dozen heparin-binding proteins that play pivotal roles in numerous cellular processes related to development and repair of tissues including brain, skin, and lungs.
The attachment of fluorescent dyes to proteins and other biomolecules is a common strategy used to better understand protein structure and function. These fluorescent proteins can be used in a broad range of applications including immunochemistry, fluorescence in situ hybridization (FISH), cell...
Fluorescent western blotting provides accurate, quantitative, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot. We offer optimized reagents, buffers and fluorescent conjugated antibodies so you can get the most from your fluorescent western blots.
Explore our protein gel options. Choose from precast polyacrylamide gel electrophoresis (PAGE) chemistries designed for specific applications including broad range, high, or low molecular weight separations, SDS-PAGE, native PAGE, or IEF.