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May I store antibodies or other proteins labeled with the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) in solutions containing sodium azide? Product FAQ

Answer

We do not recommend storing antibodies or other proteins labeled with the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) in solutions containing sodium azide, as pHrodo Deep Red is not stable in the presence of sodium azide. Consider using an alternative, non-azide-based anti-microbial.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E22097

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What applications are the LanthaScreen reagents appropriate for? Product FAQ

Answer

Terbium-labeled phosphospecific antibodies have been applied to assays for over 150 kinases. Antibodies specific to epitope tags such as 6-His or GST have been applied to nuclear receptor assays. Terbium labeled streptavidin is optimal for developing protease assays using biotinylated fluorescein labeled peptides. Ubiquitin assays for E3s have been developed using labeled ubiquitin proteins and anti-epitope tags, and deubiquitination assays can be performed using our DUB substrate. See the website (thermofisher.com/lanthascreen) for data and examples.

Answer Id: E20847

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I need to label my tissue section with two mouse primary antibodies. Do I have to make direct conjugates of the antibodies to use them? Product FAQ

Answer

Check the isotype of the two antibodies to see if they are different. If so, we offer isotype-specific goat anti-mouse secondary antibodies that can be used together with minimal cross-reactivity. If the two mouse antibodies are of the same isotype, then you would need to make direct conjugates, such as with our antibody labeling kits or APEX, and SiteClick kits.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E6461

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FluoReporter FITC Protein Labeling Kit Manual / Product Insert

  • Version: 03-29-2010
Catalog # F6434

Can I use 50 μg of protein with Fluorescent Protein Labeling Kits? Product FAQ

Answer

No. We recommend using 1 mg of protein with Fluorescent Protein Labeling Kits. For smaller protein sample sizes, we recommend using Microscale Protein Labeling kits which are optimized for 20-100 µg of protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E21443

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Pacific Orange Protein Labeling Kit Manual / Product Insert

  • Version: 10-12-2005
Catalog # P30016(Discontinued)

Quick Ref: Protein Labeling Kits Manual / Product Insert

  • Version: MAN0009630 Rev A (11 January 2017)
Catalog #
  • P30016(Discontinued)
  • A10171(Discontinued)

Important customer notification - purification resin in the Antibody and the Protein Labeling kits Manual / Product Insert

  • Version: A.0
Catalog #

User Guide: Protein Labeling Kits (For Alexa Fluor, Pacific Blue, Fluorescein-EX, and Oregon Green 488) Manual / Product Insert

  • Version: A.0
Catalog #

Biotin-XX Microscale Protein Labeling Kit Manual / Product Insert

  • Version: B30757 B30758 (06-26-2006)
Catalog #

DSB-X Biotin Protein Labeling Kit Manual / Product Insert

  • Version: 10-10-2001
Catalog # D20655

Which LanthaScreen antibody should I use? Product FAQ

Answer

For tyrosine kinase assays, we offer four different terbium-labeled anti-phosphotyrosine antibodies. When using fluorescein-labeled poly-GAT or poly-GT, performance using the Tb-labeled PY20 antibody is excellent. When using specific peptide substrates, performance may vary between the different varieties of anti phosphotyrosine antibodies, and as such we recommend evaluating the assay with a selection of antibodies in order to find the antibody that gives optimal performance. However, for tyrosine kinase assays, any of the anti-phosphotryrosine antibodies will likely perform well.

For serine/threonine kinases, we provide a reactivity chart at thermofisher.com/lanthascreen which shows the performance of various kinases using suitable substrate/antibody pairs.

Answer Id: E20854

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Can you offer some tips for choosing a secondary antibody? Product FAQ

Answer

Secondary antibodies may be either too specific (e.g., recognize only one host species of primary antibody) or too general (e.g., recognize whole IgG and any fragments thereof). In most cases, these limitations can be overcome by carefully designing the experimental system and choosing the appropriate secondary antibody. The following considerations are useful to help choose a secondary antibody:

Determine the host species of the primary antibody (e.g., mouse anti-tubulin).
Select an appropriate host species for the secondary antibody-you will need a secondary antibody that is raised in a species different from the host species of the primary antibody (e.g., goat anti-mouse IgG).
Consider cross-reactivity or specificity issues of the secondary antibody.
Highly cross-absorbed-for multiple-labeling applications or when using samples with endogenous antibodies.
Specificity-binds to correct fragments, classes, or chains of the primary antibody.
Use an appropriate detection or purification method.
Label-appropriately conjugated to the correct enzyme, tag, or fluorophore for the chosen detection method.
Ability to bind to Protein A, Protein G, or Protein L-make sure the secondary antibody chosen has sufficient affinity for the molecules used upstream or downstream (i.e., Protein A-coated microplates).
Consider requirements of the supplied secondary antibody.
Supplied state-sterile liquid or lyophilized, suspended in PBS or Tris buffer, contains carrier proteins such as gelatin or albumin or the addition of stabilizers such as sucrose or microbial inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E12596

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Novel Solid-phase Antibody Labeling Using APEX Antibody Labeling Kits Manual / Product Insert

  • Version: B30757 B30758 09-18-2009
Catalog #
  • B30010
  • F6347
  • F10240
  • F6434
  • Z25155
  • O10241
  • A20180
  • A20184
  • A20185
  • A20182
  • A20183
  • A20186
  • A20187
  • A20170
  • A20173
  • A20171
  • A20172
  • A10236
  • A10237
  • A10238
  • S30046
  • S30045
  • A10239
  • D20655
  • A30006
  • A30007
  • A30008
  • A30009
  • F30751
  • P30013
  • P30014
  • Z25080(Discontinued)
  • F20650(Discontinued)
  • Z25405(Discontinued)
  • Z25009(Discontinued)
  • Z25605(Discontinued)
  • Z25204(Discontinued)
  • Z25104(Discontinued)
  • Z25602(Discontinued)
  • Z25206(Discontinued)
  • Z25106(Discontinued)
  • Z25200(Discontinued)
  • Z25221(Discontinued)
  • Z25342(Discontinued)
  • Z25045(Discontinued)
  • Z25001(Discontinued)
  • Z25400(Discontinued)
  • B30756(Discontinued)
  • Z25022(Discontinued)
  • Z25341(Discontinued)
  • P30016(Discontinued)
  • Z25170(Discontinued)
  • Z25607(Discontinued)
  • Z25310(Discontinued)
  • Z25210(Discontinued)
  • Z25257(Discontinued)
  • Z25256(Discontinued)
  • Z25251(Discontinued)
  • Z25110(Discontinued)
  • T10244(Discontinued)
  • Z25252(Discontinued)
  • Z25010(Discontinued)
  • Z25280
  • A-10235
  • Z25180
  • Z-25160
  • A-10171
  • Z-25003
  • Z-25020
  • Z-25042
  • Z-25100
  • Z25606
  • Z25301
  • Z25460
  • Z25121
  • Z25270
  • Z-25070
  • Z25090
  • Z-25113
  • Z-25454
  • Z-25031
  • Z-25154
  • Z-25254
  • Z-25205
  • A-20181
  • Z-25207
  • Z25350
  • Z25470
  • Z25030

User Guide: pHrodo iFL Microscale Protein Labeling Kits Manual / Product Insert

  • Version: MAN0017102 Rev A.0 (21 June 2017)
Catalog #
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