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Application Note: Comprehensive Protein Glycosylation Comparison of an Innovator Monoclonal Antibody to a Candidate Biosimilar by HILIC UHPLC Analysis Product Literature

Molecular characterization and expression analysis of the Rab GTPase family in Vitis vinifera reveal the specific expression of a VvRabA protein Citations & References

  • Authors: Abbal, P; Pradal, M; Muniz, L; Sauvage, FX; Chatelet, P; Ueda, T; Tesniere, C
  • Journal: JOURNAL OF EXPERIMENTAL BOTANY 2008 9:2403-2416

Data Sheets: Standardized Assays and Reagents for GeneChip Expression Analysis Product Literature

Drug-induced alterations to gene and protein expression in intestinal epithelial cell 6 cells suggest a role for calpains in the gastrointestinal toxicity of nonsteroidal anti-inflammatory agents. Citations & References

  • Authors: Raveendran NN, Silver K, Freeman LC, Narvaez D, Weng K, Ganta S, Lillich JD
  • Journal: J Pharmacol Exp Ther 2008; (325):2 389-399
  • PubMed ID: 18281595

Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-phthaldialdehyde/2-mercaptoethanol reagent. Citations & References

  • Authors: Lee KS, Drescher DG
  • Journal: J Biol Chem (1979) 254:6248-6251
  • PubMed ID: 109447
Catalog # P2331MP(Discontinued)

Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2′-deoxycytidine, in esophageal squamous cell carcinoma Citations & References

  • Authors: Arai, M; Imazeki, F; Sakai, Y; Mikata, R; Tada, M; Seki, N; Shimada, H; Ochiai, T; Yokosuka, O
  • Journal: ONCOLOGY REPORTS 2008 2:405-412

Rapid staining methods for analysis of deoxyribonucleic acid and protein in mammalian cells. Citations & References

  • Authors: Crissman HA, Oka MS, Steinkamp JA
  • Journal: J Histochem Cytochem (1976) 24:64-71
  • PubMed ID: 56392
Catalog #

Can streptavidin conjugated WesternDot reagents be used for northern or Southern blot analysis? Product FAQ

Answer

This may be possible, but has not been validated. A biotinylated probe should be detected with any of our streptavidin labeled Qdot probes (such as our Qdot 625 streptavidin conjugate, Cat. No. Q22063) using standard northern or Southern blotting protocols.

Answer Id:: E10872

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N-isopropyliodoacetamide in the reduction and alkylation of proteins: use in microsequence analysis. Citations & References

  • Authors: Krutzsch HC, Inman JK
  • Journal: Anal Biochem (1993) 209:109-116
  • PubMed ID: 8465942

Gene transcription analysis of Saccharomyces cerevisiae exposed to neocarzinostatin protein-chromophore complex reveals evidence of DNA damage, a potential mechanism of resistance, and consequences of prolonged exposure. Citations & References

  • Authors: Schaus S E; Cavalieri D; Myers A G;
  • Journal: Proc Natl Acad Sci U S A (2001) 98:11075-11155
  • PubMed ID: 11562456
Catalog # 10481018(Discontinued)

What is the basic protocol for using M-PER Mammalian Protein Extraction Reagent with suspension cells? Product FAQ

Answer

Add M-PER Reagent to the cell pellet that has been washed once in PBS. Use at least 1 mL of M-PER Reagent for each 100 mg (~100 µL) of wet cell pellet. For example, 2 × 106 HeLa cells is equal to ~20 mg of cells and requires 200 µL of M-PER Reagent. Shake mixture gently for 10 minutes. Remove cell debris by centrifugation at ~14,000 × g for 15 minutes. Transfer the supernatant to a new tube for analysis.

Answer Id:: E13402

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High level expression of the major antigenic African swine fever virus proteins p54 and p30 in baculovirus and their potential use as diagnostic reagents. Citations & References

  • Authors: Oviedo JM; Rodriguez F; Gomez-Puertas P; Brun A; Gomez N; Alonso C; Escribano JM
  • Journal: Journal of Virological Methods 1997 1:27-35
Catalog #

A method for the isolation of covalent DNA-protein crosslinks suitable for proteomics analysis. Citations & References

  • Authors: Barker S, Murray D, Zheng J, Li L, Weinfeld M,
  • Journal: Anal Biochem (2005) 344:204-215
  • PubMed ID: 16091282
Catalog # S12010
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