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Can you tell me more about the Total Exosome RNA & Protein Isolation Kit? Product FAQ

Answer

The Total Exosome RNA & Protein Isolation Kit (Cat. No. 4478545), developed specifically for exosome samples, uses an initial acid-phenol:chloroform extraction followed by a final purification over a glass-fiber filter column to provide a robust initial RNA purification. After the acid-phenol extraction, ethanol is added to the aqueous phase and then passed through a filter cartridge containing the glass-fiber filter, which immobilizes the RNA. The filter is washed, and the RNA is eluted with a low ionic-strength solution.

The kit recovers all RNA longer than approximately 10 nt through several kb, although the majority of RNA contained in purified exosomes ranges in size from 20-300 nt. The kit also contains an additional protocol for enrichment of short RNA (less than 200 nt), but we recommend the total RNA isolation protocol in order to maximize recovery of all RNA, including vario+us mRNA, rRNA, and ncRNA fragments. This kit also provides an option to recover protein from the same sample through the use of the Exosome Resuspension Solution. The kit can be used to isolate RNA and protein from exosomes purified from any sample type, using either the Total Exosome Isolation reagents or any other protocol such as ultracentrifugation.

Answer Id:: E7466

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I'd like to isolate RNA and protein from the same sample. Do you have a kit for this? Product FAQ

Answer

Yes, you can use TRIzol Reagent or the mirVana PARIS RNA and Native Protein Purification Kit.

Answer Id:: E7829

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Purification of adult hepatic progenitor cells using green fluorescent protein (GFP)-transgenic mice and fluorescence-activated cell sorting. Citations & References

  • Authors: Fujikawa T; Hirose T; Fujii H; Oe S; Yasuchika K; Azuma H; Yamaoka Y
  • Journal: Journal of Hepatology 2004 2:162-170
Catalog #
  • 4318739(Discontinued)
  • 4327058(Discontinued)
  • 4327059(Discontinued)

2020 Protein Expression User Group Presentation Part I: From Synthetic Gene to Protein Purification Product Literature

Brochure: Protein Digestion for Peptide Mapping and Quantitation: Overview of SMART Digest and SMART Digest ImmunoAffinity Kits Product Literature

SMART Digest Soluble Trypsin Kit – Smarter Protein Digestion FAQ Product Literature

User Guide: pHrodo iFL Microscale Protein Labeling Kits Manual / Product Insert

  • Version: MAN0017102 Rev A.0 (21 June 2017)
Catalog #

SMART Digest and SMART Digest ImmunoAffinity (IA) Kit Technical Guide – Smarter Protein Preparation Product Literature

User Guide: GeneChip 3' IVT PLUS Reagent Kit Manual / Product Insert

  • Version: B.0
Catalog #

User Guide: GeneChip WT Pico Reagent Kit Manual / Product Insert

  • Version: B.0
Catalog #

Does the DRR (DNase Binding Reagent) from RapidOut DNA Removal Kit bind to other proteins? Is the kit suitable for removal of other proteins from the reaction mixture? Product FAQ

Answer

We have tested the kit for DNase I removal only. Although DRR can bind to other proteins, we cannot provide any recommendations for removal of protein contaminants from the enzymatic mixtures. If the RNA sample contains a considerable amount of protein contaminants, modify the RNA purification protocol to reduce protein contamination.

Answer Id:: E8856

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