Product Literature

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Manual / Product Insert

User Guide: SuperSignal Enhan Molecular Weight Protein Ladder

Version: Jan. 2015
Catalog #

Product FAQ

What is the composition of the Spectra Multicolor Broad Range Protein Ladder?

Answer

The Spectra Multicolor Broad Range Protein Ladder is a mixture of 10 recombinant prestained proteins ranging from 10 kDa to 260 kDa, for use in gel electrophoresis and western blotting. Four different chromophores are bound to the proteins, producing a brightly colored ladder.

Answer Id: E11663

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Product FAQ

What is the composition of the Spectra Multicolor High Range Protein Ladder?

Answer

The Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300 kDa) for use as a high-MW standard in gel electrophoresis and western blotting. Three different chromophores are bound to the proteins, producing a brightly colored ladder.

Answer Id: E11664

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Product FAQ

What is the composition of the Spectra Multicolor Low Range Protein Ladder?

Answer

The Spectra Multicolor Low Range Protein Ladder is a prestained mixture of six recombinant proteins (1.7 to 40 kDa) for use in gel electrophoresis and western blotting. Three different chromophores are bound to the proteins, producing a brightly colored ladder.

Answer Id: E11665

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Manual / Product Insert

Protocol: BenchMark Pre-Stained Protein Ladder

Version: MAN0007901 Rev.B 18Mar2014
Catalog #

Product FAQ

Are any animal-derived proteins present in the Spectra Protein Ladders?

Answer

The Spectra Protein Ladders contain recombinant prokaryotic proteins and do not contain any animal-derived proteins.

Answer Id: E11666

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Product FAQ

Could a protein ladder be used on 6% Tris-glycine SDS-PAGE, although 4-20% gel is recommended? What effect would it have if the ladder is run on such a low concentration gel?

Answer

Protein ladders can be run on lower percentage gels than we recommend, however it should be expected that several bands of lower molecular weight proteins will run out of the gel if the gel is run until the dye front reaches the bottom of the gel. In case of shorter electrophoresis time it is also possible that some lower bands will not separate and will be covered by the dye front. In addition, lower molecular weight protein bands may look diffused. The lower percentage gels can be used in cases when the customer is interested in visualization of large proteins only.

Answer Id: E8791

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Manual / Product Insert

Product Info Sheet: iBright Prestained Protein Ladder

Version: MAN0016264 Rev. A.0 (01Mar2017)

Product FAQ

With the iBright Prestained Protein Ladder, I am unable to detect the 2 unstained protein bands. Why is this?

Answer

Primary antibodies with low starting concentrations may result in insufficient chemiluminescent detection of the western blot positive control bands. If the unstained 30 kDa and 80 kDa bands produce weak or no signal, spike the diluted primary antibody with the corresponding rabbit IgG or mouse IgG to a concentration of 1-5 µg/mL, prior to secondary antibody incubation. Follow with respective secondary (GAM/GAR) incubation to increase the intensity of western blot positive control bands in the iBright Prestained Protein Ladder.

Answer Id: E16156

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Manual / Product Insert

Protocol: BenchMark Unstained Protein Ladder

Version: MAN0007917 Rev.B 18Mar2014
Catalog #

Product FAQ

What are the storage conditions and shelf life for the MagicMark XP Western Protein Standard?

Answer

We recommend storing the MagicMark XP Western Protein Standard -20 degrees C in aliquots to avoid repeated freezing and thawing. It is stable for 4 months when properly stored.

Answer Id: E11739

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Product FAQ

What are the storage conditions and shelf life for the PageRuler Unstained Protein Ladders?

Answer

We recommend storing the PageRuler Unstained Protein Ladders at -20 degrees C where they are stable for a year.

Answer Id: E11684

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Product FAQ

What are the storage conditions and shelf life for the HiMark Prestained Protein Standard?

Answer

We recommend storing the HiMark Prestained Protein Standard at -20 degrees C. The ladder is stable for 4 months when properly stored.

Answer Id: E11667

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Manual / Product Insert

User Guide: Spectra Multicolor High Range Protein Ladder

Version: Jan. 2015
Catalog #