Manual / Product Insert

User Guide: Pierce Pull-Down Biotinylated Protein:Protein Interaction Kit

Version: Jan. 2015
Catalog #

Manual / Product Insert

User Guide: Pierce Far-Western Biotinylated-Protein:Protein Interaction Kit

Version: Jan. 2015
Catalog #

Manual / Product Insert

User Guide: Pierce Pull-Down PolyHis Protein:Protein Interaction Kit

Version: Jan. 2015
Catalog #

Product FAQ

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

Answer

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Answer Id: E8662

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Manual / Product Insert

ProtoArray Protein Microarray PPI Kits for Biotinylated Proteins

Version: Version D, 18 October 2006
Catalog #
  • PA011(Discontinued)
  • PA0121011(Discontinued)
  • PAH0524011(Discontinued)

Manual / Product Insert

ProtoArray Protein Microarray PPI Kits for Epitope-Tagged Proteins

Version: Version D, 18 October 2006
Catalog #
  • PA0121013(Discontinued)
  • PA013(Discontinued)
  • PAH0524013(Discontinued)

Product Literature

Thermo Scientific Avidin-Biotin Technical Handbook

Manual / Product Insert

User Guide: Pierce qIP Protein Interaction Tluc Assay Reagents

Version: Jan. 2015
Catalog #
  • 82015(Discontinued)
  • 82016(Discontinued)

Manual / Product Insert

User Guide: Pierce Magnetic c-Myc-Tag IP/Co-IP Kit

Version: MAN0016845 Rev, A.0 (23Feb2017)
Catalog #

Manual / Product Insert

User Guide: Pierce c-Myc Tag IP/Co-IP Kit

Version: Jan. 2015
Catalog #

Manual / Product Insert

User Guide: Pierce Anti-c-Myc Magnetic Beads

Version: Jan. 2015

Product FAQ

What types of Thermo Scientific Protein:Protein Interaction (PPI) kits are available?

Answer

There are Pull-Down Kits and Far-Western Kits. The Pull-Down Kits are very similar to an immunoprecipitation (IP), but instead of an antibody, a tagged bait protein [biotin (Cat. No. 21115) , GST (Cat. No. 21516) or 6xHis (Cat. No. 21277)] is used to bind the prey or target protein. This protein complex is then purified using an affinity column specific for the tagged bait protein. The Far Western Kits are similar to Western Blots, but again, instead of an antibody, a tagged non-immunoglobulin protein [biotin (Cat. No. 23500) or GST (Cat. No. 23505)] is used to bind to the prey or target protein for detection on the membrane.

Answer Id: E8659

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Product Literature

Protein-Protein Interaction Profiling on Invitrogen Protoarray™ High Density Protein Microarrays (application note)

Product FAQ

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

Answer

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Answer Id: E8660

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