Find useful tips and resources for rapid and high yield production of proteins in a mammalian expression system. View our FAQs related to setting up of the experiment for high-yield transient or stable protein expression, including transient expression of functional membrane proteins.
Find information about total RNA, LMW-enriched RNA and preparation for miRNA analysis.
Learn More Fluorescent Protein Labeling
Find reagents and kits for fluorescent protein labeling with Alexa Fluor dyes, FITC, Texas Red, Qdot nanocrystals, and more.
Classic click reactions involve a copper-catalyzed azide–alkyne cycloaddition  to label and detect molecules of interest in cells or tissues. A drawback of this approach is that copper ions can harm cells, reduce the fluorescence of fluorophores, and impair protein function. Our new Click-iT® DIBO reagents employ copper-free click chemistry reactions that capture or detect azide-substituted targets, including our metabolic analogs of proteins and many of their posttranslationally modified forms. The key to copper-free click chemistry is the DIBO (dibenzocyclooctyne) core of the dye-containing derivative. The strain in the eight-membered ring allows the reaction with azide-modified molecules to occur in the absence of catalysts, enabling the study of the surface of live cells, and preventing copper-induced damage of fluorescent proteins such as GFP in fixed and permeabilized cells.
Access useful troubleshooting suggestions for protein thermal shift assays including instrument setup, data analysis and software support.
Learn More Recombinant Proteins Support—Getting Started
Get tips and helpful information for setting up your experiment using our recombinant proteins (including SINO recombinant proteins) for various applications, including cell growth, differentiation, and cell signaling. View our FAQs for reconstitution, storage, and handling of recombinant proteins, and for their use in growth and differentiation of various stem cells.
Learn More Overview of Protein Labeling
This article discusses general categories of labeling of proteins, nucleic acids and other biomolecules.
Learn More Protein Labeling and Crosslinking
We offer a portfolio of protein crosslinking, modifying and labeling products, including amine-to-amine and amine-to-sulfhydryl crosslinkers, biotinylation reagents, and peg labeling products.
Learn More Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support—Getting Started
Get tips and helpful information related to your protein immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and pulldown experiments.
Study macroautophagy with Premo fluorescent-based sensors
This article describes why a researcher might choose a reagent that includes PEG, along with an overview of PEG products that are offered.
Learn More Microplate Assays for Protein Quantitation
Quantitating protein samples is an important step before gel electrophoresis or Western blot analysis, and for measuring bound versus free protein levels in protein binding assays. The choice of assay format is often based on other components in the sample mixture. NanoOrange® is compatible with reducing agents and is sensitive enough that many contaminants can be diluted out below threshold levels. CBQCA is compatible with lipoproteins or lipid protein mixtures.
Learn More Carbodiimide Crosslinker Chemistry
For coupling to proteins or other molecules that contain carboxyl groups.
Learn More Avidin-Biotin Interaction
A review of the Avidin-biotin interaction used for protein detection, isolation and purification, including content on streptavidin and Neutravidin Protein and common research applications.
Learn More Fluorescein (FITC)
See the characteristics of fluorescein and the range of antibody conjugates, kits, and other products related to this fluorophore.