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RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein

RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.

RAF1/c-RAF Polyclonal Antibody (Bethyl Laboratories)

RAF1/c-RAF Polyclonal Antibody for Western Blot

RAF1/c-RAF Polyclonal Antibody (Invitrogen™)

RAF1/c-RAF Polyclonal Antibody for Western Blot, Flow

BRAF Recombinant Human Protein

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron.

BRAF [V599E] Recombinant Human Protein

BRAF acts upstream of MEK in the MAP kinase pathway and is involved in cell division, differentiation, and secretion. The BRAF mutant V599E is oncogenic and found in a variety of cancers.

Phospho-c-Raf pTyr340 / pTyr341 Antibody (Invitrogen™)

Phospho-c-Raf (Tyr340, Tyr341) Polyclonal Antibody for Western Blot, IHC

CellSensor™ AP-1-bla A375 Cell Line

The CellSensor® AP1-bla A375 cell line contains a beta-lactamase reporter gene under control of the AP1 response element stably integrated into A375 cells. A375 cells are human melanoma cancer cells that contain the endogenous B-Raf mutation V600E resulting in constitutive B-Raf kinase activity. The CellSensor® AP1-bla A375 cell line is a clonal population isolated by flow cytometry based on constitutive expression of beta-lactamase. This cell line has been validated with various small-molecule Raf inhibitors as well as B-Raf Stealth RNAi™ siRNA. This cell line has also been tested for assay performance under variable conditions, including DMSO concentration, cell number, compound incubation time, and substrate loading time and validated for Zfi-factor and IC50 concentrations of Raf1 Inhibitor I. The RAF gene family (RAF1, A-RAF, and B-RAF) encodes closely related serine/threonine protein kinases that are important effectors of Ras activation. Raf1 and A-Raf are rarely mutated, whereas mutations in B-Raf are common in human cancers, especially melanoma. B-Raf is mutated in about 70% of human melanomas, 35-70% of papillary thyroid carcinomas, and less commonly in lung and colorectal carcinomas. Mutations are mostly in the B-Raf kinase domain and, in melanomas, the vast majority are V600E missense mutations leading to activation of B-Raf kinase. The constitutive activity of B-Raf V600E can directly lead to the activation of the Mek/MAPK signaling pathway. Therefore, inhibition of B-Raf/Mek/MAPK signaling could be a potential way of treating melanomas and other tumors with mutant B-Raf.

LanthaScreen™ Tb-pMAP2K1 (pSer217/221) Antibody Kit

A member of the MAP Kinase family, MAP2K1 (mitogen-activated protein kinase kinase 1) is phosphorylated at Ser217/221 by a range of MAPK family members including Raf and B-Raf. For use in combination with the Fluorescein-MAP2K1 physiological protein substrate (PV4812) to evaluate inhibitors in medium to high throughput screening applications.

Phospho-c-Raf (Ser338, Tyr340) Polyclonal Antibody (Invitrogen™)

Phospho-c-Raf (Ser338, Tyr340) Polyclonal Antibody for Western Blot

Phospho-c-Raf (Ser259) Polyclonal Antibody (Invitrogen™)

Phospho-c-Raf (Ser259) Polyclonal Antibody for Western Blot, IF, ICC

Fluorescein-MAP2K1

Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members.

For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening applications.

Phospho-c-Raf (Ser621) Polyclonal Antibody (Invitrogen™)

Phospho-c-Raf (Ser621) Polyclonal Antibody for Western Blot

c-Raf Polyclonal Antibody (Invitrogen™)

c-Raf Polyclonal Antibody for Western Blot

Phospho-c-Raf (Tyr341, Ser339) Monoclonal Antibody (pS338[DPR/2/2A/3A]) (Invitrogen™)

Phospho-c-Raf (Ser338) Monoclonal Antibody for IHC (P)

Phospho-c-Raf (Ser301) Polyclonal Antibody (Invitrogen™)

Phospho-c-Raf (Ser301) Polyclonal Antibody for Western Blot