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What is the lowest amount of RNA that can be amplified using the Arcturus Paradise Plus RNA Extraction and Isolation Kit? Product FAQ

Answer

With this kit, you can amplify as little as 5 ng of RNA isolated from formalin-fixed, paraffin embedded (FFPE) tissues.

Answer Id: E9845

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Do you offer isolated, purified RNA? Product FAQ

Answer

Yes, please visit this website (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/purified-rna.html) for our FirstChoice total RNA isolated from human tissues, as well as from E. coli and human cell lines.

Answer Id: E7824

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Brochure: RNA isolation and purification (North America) Product Literature

Brochure: RNA isolation and purification (non-North America) Product Literature

User Guide: Arcturus® RiboAmp® HS PLUS (RNA Amplification Kit) Manual / Product Insert

  • Pub. No.: e4c0f0b2294bbb5eb537b2fc915322c96f49b50c
  • Version: 1267200 Rev. F (Feb. 2011)
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I would like to isolate microRNA, and I see that you offer several kits for this. Which one would you recommend for fresh tissue samples? For frozen tissue? For cells? Product FAQ

Answer

Please use the selection guide (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/micro-rna-and-small-rna-isolation.html) to compare our kits for miRNA isolation.

Answer Id: E7885

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I am interested in isolating mRNA. Can I use one of your kits to isolate mRNA directly from my starting sample or do I need to isolate total RNA and enrich for mRNA? Product FAQ

Answer

Please use the selection guide (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/mrna-extraction.html) to view the kits we offer for mRNA purification, as well as the differences between them.

Answer Id: E7883

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What is the difference between the RiboPure Blood Purification Kit (Cat. No. AM1928) and Mouse RiboPure Blood RNA Isolation kit (Cat. No. AM1951)? Product FAQ

Answer

The former (Cat. No. AM1928) is for isolation of RNA from human blood and the latter (Cat. No. AM1951) is for isolation of RNA from mouse and rat blood. Both kits have the same columns but differ in protocol and the wash 1 buffer, wash 2/3 buffer, and sodium acetate solutions. We do not recommend using the mouse kit for human blood samples. The mouse kit accommodates the difference in viscosity of mouse/rat vs. human blood and also the higher mouse and rat blood RNA yield. The mouse kit allows recovery of either total RNA containing small RNAs or total RNA depleted of small RNAs based on which protocol in the manual was followed. With the RiboPure Blood Purification Kit, using the standard protocol in the manual, you can isolate total RNA depleted of small RNAs, but with an alternate protocol, you can isolate total RNA containing small RNAs. Alternatively, if you need small RNAs alone, then you should follow the above alternate protocol for isolation of total RNAs containing small RNAs, and then follow Section IVA in the mirVana miRNA Isolation Kit manual (Cat. No AM1560).

Answer Id: E7844

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Can I use the Cells-to-CT kits with plasma samples? Product FAQ

Answer

We have not tested the compatibility of Cells-to-CT kits with plasma samples and would not recommend using this kit for plasma samples. Please refer to the following table to select an RNA purification kit based on your sample type: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction.html.

Answer Id: E20248

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Where can I find scripts for the MagMAX Express-96 instrument? Product FAQ

Answer

The MagMAX Express-96 protocol scripts can be found on the MagMAX Express protocol page (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction/magmax-technology/magmax-particle-processors/magmax-instrument-protocols-scripts.html). If the specific script you are looking for is not located here or on the product page for the MagMAX kit, please contact Technical Support at techsupport@thermofisher.com.

Answer Id: E20312

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Where can I find the MagMAX Express-96 scripts for the animal health MagMAX kits? Product FAQ

Answer

If the .kf2 scripts are not already loaded on the instrument, you can find them here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction/magmax-technology/magmax-particle-processors/magmax-instrument-protocols-scripts.html). The scripts for the MagMAX CORE Nucleic Acid Purification Kit can be found here (https://www.thermofisher.com/order/catalog/product/A32702).

Answer Id: E16391

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What can I use to extract DNA from FFPE (formalin fixed paraffin embedded) samples? Product FAQ

Answer

We offer 2 kits: RecoverAll Total Nucleic Acid Isolation Kit for FFPE and MagMAX FFPE DNA/RNA Ultra Kit Read more about the differences between these kits here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/dna-extractions-working-with-ffpe-samples.html).

Answer Id: E7673

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What is the column cut-off size for the RiboPure RNA Purification Kits? Product FAQ

Answer

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

Answer Id: E7840

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Do I have to purify RNA before the qPCR reaction? Product FAQ

Answer

It is most common to purify the RNA from your sample before proceeding with the next steps in a qPCR reaction. However, depending on your sample type, you may be able to skip the purification process and go directly from a lysate to the reverse transcription step. When used with compatible cell lines, Cells-to-CT kits allow you to streamline your workflow. You can find more information on how this works here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction/cells-to-ct-kits.html?icid=fr-cellstoct-main%20http:/www.thermofisher.com/us/en/home/life-science/dna-rna-purification).

Answer Id: E7481

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I am getting the same amplification in my no-RT controls as in my samples. What does this mean? Product FAQ

Answer

It means that you are probably detecting genomic DNA (gDNA) in your sample. It could be the method you are using to prepare your RNA. Our website has information and products to help you eliminate genomic DNA contamination. Also, if you are using an assay ID with the suffix _s, it will, by definition, detect gDNA. Assays ending with the suffix _s are designed within a single exon. If there is gDNA present in the sample, you must treat with DNase.

Answer Id: E2784

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