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Product Bulletin: TaqMan® Gold RT-PCR Kit: Real-Time PCR Reagents Manual / Product Insert

  • Version: AB
Catalog #
  • N8080228(Discontinued)
  • 4304441(Discontinued)
  • 402930(Discontinued)
  • 4304439(Discontinued)
  • 402876
  • 402823-LSG
  • 4367218-LSG

Can I create my own calibration plates? Product FAQ

Answer

You can create your own background calibration plate using the directions in the ViiA 7 Real-Time PCR System Maintenance Guide (Appendix C) http://tools.thermofisher.com/content/sfs/manuals/4442661C.pdf. For all other calibration plates, you can purchase a calibration kit, or the individual plates, as required (http://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/real-time-calibration-kits/viia-7-system-calibration-kits.html).

Answer Id:: E7187

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Brochures & Specifications: TaqMan® Gold RT-PCR Kit: Real-Time PCR Reagents: Product Bulletin (English ) Product Literature

Is there a TaqMan PCR Core Reagents kit for the Fast Real-Time PCR System? Product FAQ

Answer

No. The only Fast reagents that we offer are the TaqMan Fast Universal PCR Master Mix (2X) for single plex use, and Fast Advanced Master Mix for greater sensitivity and multiplexing use.

Answer Id:: E2348

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Is my real-time PCR instrument compatible with HRM? Product FAQ

Answer

Among our Applied Biosystems real-time PCR instruments, the following are compatible with HRM:

- 7500 Fast Real-Time PCR System
- 7900HT Fast Real-Time PCR System
- StepOne and StepOnePlus Real-Time PCR Systems
- ViiA 7 Real-Time PCR System
- QuantStudio 6 and 7 Real-Time PCR Systems
- QuantStudio 12K Flex Real-Time PCR System
- QuantStudio 3 and 5 Real-Time PCR Systems

Answer Id:: E7562

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Event-specific Detection Methods for Genetically Modified Maize MIR604 Using Real-time PCR Citations & References

  • Authors: Kim, JH; Kim, HY
  • Journal: FOOD SCIENCE AND BIOTECHNOLOGY
Catalog #

Comparative evaluation of real-time PCR and conventional RT-PCR during a 2 year surveillance for influenza and respiratory syncytial virus among children with acute respiratory infections in Kolkata, India, reveals a distinct seasonality of infection Citations & References

  • Authors: Agrawal, AS; Sarkar, M; Chakrabarti, S; Rajendran, K; Kaur, H; Mishra, AC; Chatterjee, MK; Naik, TN; Chadha, MS; Chawla-Sarkar, M
  • Journal: JOURNAL OF MEDICAL MICROBIOLOGY
Catalog #

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan® real-time RT-PCR assays for respiratory pathogens Citations & References

  • Authors: Yang, G; Erdman, DD; Tondella, ML; Fields, BS
  • Journal: JOURNAL OF VIROLOGICAL METHODS
Catalog #

My amplification curves have a funny shape in my qPCR experiment. What is causing this? Product FAQ

Answer

There are several reasons that amplification could be delayed. Please see the information in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/abnormal-amplification-curves/amplification-occurs-later.html) for more details.

Answer Id:: E7505

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What can I do if the amplification of my target gene is later than expected for my qPCR experiment? Product FAQ

Answer

There are several reasons that amplification could be delayed. Please see the information in our Real-Time Troubleshooting Tool for more details (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/abnormal-amplification-curves/amplification-occurs-later.html).

Answer Id:: E7504

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I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this? Product FAQ

Answer

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

Answer Id:: E7506

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How do I perform the HRM calibration on my real-time PCR instrument? Product FAQ

Answer

The HRM calibration is a 3-step procedure. In some instrument software, the calibration process has been streamlined to a single-step procedure (applies to the ViiA7 Real-Time PCR System and QuantStudio Real-Time PCR Systems).

For the HRM calibration, the first step is a PCR reaction to generate the HRM dye plate. The reaction plate contains a DNA template, a primer pair, and the PCR mix with HRM dye. The second step is dye calibration using the PCR plate from step 1. The last step is a melt curve run, again using the same plate. For details, please refer to the HRM guides for each instrument:

- 7500 Fast Real-Time PCR System (with software version 1.4 or 2.0.4 and below) (https://tools.thermofisher.com/content/sfs/manuals/cms_050347.pdf)
- 7500 Fast Real-Time PCR System (with software version 2.0.5 and above) (https://tools.thermofisher.com/content/sfs/manuals/4457847.pdf)
- 7900HT Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/cms_050347.pdf)
- StepOne and StepOnePlus Real-Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/4457847.pdf)
- ViiA 7 Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/4443531.pdf)
- QuantStudio 6 and 7 Real-Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/4489823.pdf)
- QuantStudio 3 and 5 Real Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/MAN0014394_HighResMeltExperiment_GSG.pdf)
- QuantStudio 12K Flex Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/4470692A.pdf)

Answer Id:: E7572

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Unique effects of KIT D816V in BaF3 cells: Induction of cluster formation, histamine synthesis, and early mast cell differentiation antigens Citations & References

  • Authors: Mayerhofer, M; Gleixner, KV; Hoelbl, A; Florian, S; Hoermann, G; Aichberger, KJ; Bilban, M; Esterbauer, H; Krauth, MT; Sperr, WR; Longley, JB; Kralovics, R; Moriggl, R; Zappulla, J; Liblau, RS; Schwarzinger, I; Sexl, V; Sillaber, C; Valent, P
  • Journal: JOURNAL OF IMMUNOLOGY
Catalog #

Up-regulation of survivin mRNA might be a marker for non-invasive detection of non-small cell lung cancer rather than for prognosis Citations & References

  • Authors: Warnecke-Eberz, U; Baldus, SE; Bollschweiler, E; Hoelscher, AH; Metzger, R
  • Journal: ANTICANCER RESEARCH
Catalog #

Molecular mechanisms of the antiangiogenic and antitumor effects of mycophenolic acid Citations & References

  • Authors: Domhan, S; Muschal, S; Schwager, C; Morath, C; Wirkner, U; Ansorge, W; Maercker, C; Zeier, M; Huber, PE; Abdollahi, A
  • Journal: MOLECULAR CANCER THERAPEUTICS
Catalog #
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