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- Authors: Bruno JG,
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- PubMed ID: 25437803
What is the ideal range of protein concentration needed for using Pro-Detect assay kits? Product FAQ
For sandwich assays, the range is 0.1-10 µg/mL of pure tagged protein.
Note: The lateral flow assays can detect outside of this range; however, intensity of the bands will be lower at concentrations higher and lower than the working range. It is not recommended to use at concentrations less than 0.01 µg/mL. In a mixture of proteins such as lysates, the target protein has to be in the detectable range (0.1-10 µg/mL) for the assay to be successful.
For competition assays, the range is 4-20 µg/ml.
Note: The competitive lateral flow assays can detect down to 1 µg/mL, but loss of band intensity may be less distinguished. As there is no upper limit, the lateral flow strip will continue to show positive results at concentrations greater than 20 µg/mL, visualized by complete loss of test lines (test lines closest to the sample will disappear first). It is not recommended to use at concentrations less than 1 µg/mL.
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