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Pierce™ SDS-PAGE Sample Prep Kit (Thermo Scientific™)

The Thermo Scientific Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

Features of the SDS-PAGE Sample Prep Kit:

Eliminates gel artifacts caused by incompatible contaminants—removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis
Compatible with the BCA protein assay—allows protein quantitation of the processed sample before gel-loading
Enriches dilute protein solutions—concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes
Powerful and flexible—process 2 to 350 µL of sample containing up to 70 µg of protein; recover 75 to 85% of the total protein in 50 µL of elution buffer
Convenient, complete kit—includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Includes:
• PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

Applications:
• Inclusion bodies solubilized in guanidine·HCl
• Samples containing low-pH buffers, thiocyanates or urea
• Proteins precipitated in ammonium sulfate
• Concentration of dilute protein solutions

The SDS-PAGE Sample Prep Kit uses a unique resin composed of modified diatomaceous earth that binds protein in the presence of DMSO. The simple protocol involves combining 2 to 350 µL of sample, containing up to 70 µg of protein, with 20 µL of the PAGEprep Resin in DMSO (supplied). Proteins in the sample bind to the resin in 3 to 5 minutes, after which the nonbound contaminating chemicals are washed away. Finally, the purified proteins are recovered from the resin in 50 µL of Elution Buffer. The recovered protein sample is then ready to mix with the supplied 5X Sample Loading Buffer for gel loading (or another sample loading buffer). The procedure also allows for dilute protein samples to be concentrated up to six-fold, enabling more protein to be loaded per well. In addition, the Thermo Scientific Pierce BCA Protein Assay (Part No. 23225) is compatible with the Elution Buffer and may be used to determine final protein concentration before gel loading.

For reliable SDS-PAGE analysis, protein samples must be prepared in a buffer free of interfering substances and at protein concentrations adequate for analysis. Many interfering substances, which interfere with typical sample buffers for polyacrylamide gel electrophoresis (SDS-PAGE), are difficult to remove by traditional sample preparation methods. For example, protein samples containing 6M guanidine-HCl will precipitate when mixed with Laemmli buffer for SDS-PAGE, causing the sample to run poorly on a gel. Fortunately, samples containing a wide range of interfering chemicals, such as chaotropic agents, detergents, lipids, pH extremes and salts, can be cleaned up within minutes using the SDS-PAGE Sample Prep Kit.

Related Products
Pierce™ SDS-PAGE Sample Prep Kit

Sodium Dodecyl Sulfate (SDS), Lauryl (Thermo Scientific™)

Thermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE).

Features of Sodium Dodecyl Sulfate (Lauryl):

• Popular anionic detergent for a variety of protein methods
• Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE)
• Common component of cell lysis buffers

This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. The formulation is a mixture of several different alkyl sulfate chain lengths (C10 to C18).

Properties of SDS (values for pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate:
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless to slightly yellow.
• Chain length: C12 >60%; C14 = 20 to 35%; C16 <10%; C10 and C18 <1% each

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Sodium Dodecyl Sulfate (SDS), C12
Sodium Dodecyl Sulfate (SDS), 20% Solution

Novex™ Tricine SDS Running Buffer (10X) (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.

Pierce™ 20X Tris-HEPES-SDS Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X Tris-HEPES-SDS Buffer is a space-saving stock solution used for preparing the Tris-HEPES-SDS running buffer required for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise and Pierce Precast Gels.

Features of Thermo Scientific Pierce 20X Tris-HEPES-SDS Buffer:

Tris-HEPES-SDS buffer—diluted 20-fold in water, the solution yields 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Tris-HEPES-SDS Buffer makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8, when diluted to 1X with water. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

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BupH™ Tris-HEPES-SDS Running Buffer

20X Bolt™ MES SDS Running Buffer (Invitrogen™)

Bolt® buffers and sample reducing agents are optimized for use with Bolt® Bis-Tris Plus gels, and are available in a variety of formats. Note: Bolt® gel run times may vary depending upon the type of running buffer used.

About Bolt® Bis-Tris Plus Gels
Bolt® Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE® Bis-Tris gels, Bolt® Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt® gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt® gels to obtain optimal results for your day-to-day protein separation needs. Bolt® Bis-Tris Plus gels come in four gel types and multiple well formats. A Mini Gel Tank is required for use of all Bolt® gels.

BupH™ Tris-HEPES-SDS Running Buffer (Thermo Scientific™)

Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

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Pierce™ 20X Tris-HEPES-SDS Buffer

Novex™ Tris-Glycine SDS Sample Buffer (2X) (Invitrogen™)

Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended Buffers: By choosing the appropriate Novex® pre-mixed buffer, you can create either native, denaturing or reducing running conditions with any Novex® Tris-Glycine Gel.

7300 System SDS v1.4 Software Upgrade Kit (Applied Biosystems™)

This software upgrades earlier versions of the 7300 SDS Software to provide maximum capability and reliability. Powerful and user-friendly, the 7300 Sequence Detection Software includes:
• Simple plate setup wizard is streamlined for each application – option to setup before or after the completing the run
• Correct plate-setup mistakes after the run without losing collected data.
• One-click graphical export to PowerPoint or JPG for top notch presentations
• Flexible system can be used as a plate reader or a regular thermal cycler if needed
• Real-time amplification monitoring and flexibility to add additional PCR cycles during a run
• Lamp-lifetime monitoring ensures highest possible instrument performance

For Research Use Only. Not for use in diagnostics procedures.

Novex™ Tricine SDS Sample Buffer (2X) (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.

7300 System SDS v1.4 Upgrade Kit with RQ Study (Applied Biosystems™)

This software includes an upgrade for the 7300 SDS Software to provide the capability and reliability of the most recent version. Powerful and user-friendly, the 7300 Sequence Detection Software includes:
• Simple plate setup wizard is streamlined for each application – option to setup before or after the completing the run
• Correct plate-setup mistakes after the run without losing collected data
• One-click graphical export to PowerPoint or JPG for top notch presentations
• Flexible system can be used as a plate reader or a regular thermal cycler if needed
• Real-time amplification monitoring and flexibility to add additional PCR cycles during a run
• Lamp-lifetime monitoring ensures highest possible instrument performance

Additionally, the 7300 System SDS RQ Study Software, a gene expression analysis software package with powerful data viewing capabilities, is included. Data from up to 10 96-well RQ plates can be simultaneously analyzed. In an RQ study, you can:
• Select the endogenous control and the calibrator sample.
• Select the control type when applicable.
• Set baseline and threshold values and RQ Min⁄Max
Confidence Levels.
• Omit individual wells or sample replicates.

The user is able to view post-run amplification in three different formats, while gene expression plots show expression levels of target samples relative to the calibrator.

For Research Use Only. Not for use in diagnostics procedures.

NuPAGE™ MOPS SDS Running Buffer (20X) (Invitrogen™)

NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels only. NuPAGE® MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with NuPAGE® Novex® Bis-Tris gels. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Get consistent, convenient, high-quality results
Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution and require simple dilution with deionized water before use.

Related links

Compare protein migration patterns with different combinations of gels and running buffers.

Find NuPAGE® Novex® Bis-Tris gels.

Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675 (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.

NuPAGE™ MES SDS Running Buffer (20X) (Invitrogen™)

NuPAGE® MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels only. NuPAGE® MES SDS Running Buffer is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with NuPAGE® Novex® Bis-Tris gels. Use of MES buffer allows proteins to run faster than when using MOPS buffer.

Get consistent, convenient, high-quality results
Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution and require simple dilution with deionized water before use.

Related links

Compare protein migration patterns with different combinations of gels and running buffers.

Find NuPAGE® Novex® Bis-Tris gels.

NuPAGE™ Tris-Acetate SDS Running Buffer (20X) (Invitrogen™)

NuPAGE® Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins, in their denatured state, on NuPAGE® Novex® Tris-Acetate gels. NuPAGE® Novex® Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Novex® Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.

Formulation
NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended buffers
Run NuPAGE® Novex® Tris-Acetate gels with NuPAGE® Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE® Sample Preparation Reagents with these gels. The use of NuPAGE® Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native Running and Sample Buffers should be used with NuPAGE® Novex® Tris-Acetate gels.

Easy-to-use format
Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.

SDS Spectral Calibration Kit (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™ dyes on the ABI PRISM 7700® Sequence Detection System.

The SDS Spectral Calibration kit includes 8 tubes of pure dye standards for the Applied Biosystems® ABI PRISM 7700® Sequence Detection System.

• Individual tubes containing eight dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™) are provided for quick and easy calibration of the ABI PRISM 7700® Sequence Detection System
• Individual components provide flexibility in dye standards setup



• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration


This kit enables users to calibrate the ABI PRISM 7700® Sequence Detection System quickly and easily. The system automatically collects the dye standard's spectral information as part of the instrument installation procedure. The system's application algorithm then uses the calibration information to ensure accurate data analysis.