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Sodium Dodecyl Sulfate (SDS), C12 (Thermo Scientific™)

Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.

Features of Sodium Dodecyl Sulfate (C12):

• Popular anionic detergent for protein electrophoresis and solubilization
• Component of many cell lysis buffer formulations
• Specially purified formulation is >98% C12 alkyl sulfate
• Contains low levels of hexadecyl sulfate (C16), which inhibits protein renaturation

Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. It is a component of many cell lysis buffer formulations, including RIPA buffer. Typical SDS or lauryl sulfate preparations are mixtures of several different chain lengths (C10 to C18). However, protein renaturation and other specialized methods require very specific surfactant and buffer conditions. This particular formulation of SDS is highly purified to eliminate nearly all secondary chain lengths besides C12.

Properties of Sodium Dodecyl Sulfate, (pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate (SDS), C12 (Part No. 28312):
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless and free of particulates.
• Chain length: C12 ≥98.0% if C16 ≤0.8%
• Report C10, C12, C14, C16, C18 concentrations.

Related Products
Sodium Dodecyl Sulfate (SDS), Lauryl
Sodium Dodecyl Sulfate (SDS), 20% Solution

7300 System SDS v1.4 Software Upgrade Kit (Applied Biosystems™)

This software upgrades earlier versions of the 7300 SDS Software to provide maximum capability and reliability. Powerful and user-friendly, the 7300 Sequence Detection Software includes:
• Simple plate setup wizard is streamlined for each application – option to setup before or after the completing the run
• Correct plate-setup mistakes after the run without losing collected data.
• One-click graphical export to PowerPoint or JPG for top notch presentations
• Flexible system can be used as a plate reader or a regular thermal cycler if needed
• Real-time amplification monitoring and flexibility to add additional PCR cycles during a run
• Lamp-lifetime monitoring ensures highest possible instrument performance

For Research Use Only. Not for use in diagnostics procedures.

BupH™ Tris-HEPES-SDS Running Buffer (Thermo Scientific™)

Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
Pierce™ 20X Tris-HEPES-SDS Buffer

Novex™ Tricine SDS Running Buffer (10X) (Invitrogen™)

Novex Tricine SDS Running Buffer (2X) is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Recommended buffers: Use with tricine sample buffer to obtain optimal separation with tricine SDS running buffer.

See all buffers and reagents for SDS-PAGE ›

20X Bolt™ MES SDS Running Buffer (Invitrogen™)

Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

NuPAGE™ Tris-Acetate SDS Running Buffer (20X) (Invitrogen™)

NuPAGE Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins in their denatured state on Tris-Acetate gels. NuPAGE Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer. NuPAGE Tris-Acetate gels can also be run with Novex Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.

Recommended buffers
Run NuPAGE Tris-Acetate gels with NuPAGE Tris-Acetate SDS Running Buffer and to ensure good sample reduction and band resolution, use NuPAGE LDS Sample Buffer. For native running conditions, the Tris-Glycine Native running and sample buffers should be used.

See all available buffers and reagents available for SDS-PAGE

7300 System SDS v1.4 Upgrade Kit with RQ Study (Applied Biosystems™)

This software includes an upgrade for the 7300 SDS Software to provide the capability and reliability of the most recent version. Powerful and user-friendly, the 7300 Sequence Detection Software includes:
• Simple plate setup wizard is streamlined for each application – option to setup before or after the completing the run
• Correct plate-setup mistakes after the run without losing collected data
• One-click graphical export to PowerPoint or JPG for top notch presentations
• Flexible system can be used as a plate reader or a regular thermal cycler if needed
• Real-time amplification monitoring and flexibility to add additional PCR cycles during a run
• Lamp-lifetime monitoring ensures highest possible instrument performance

Additionally, the 7300 System SDS RQ Study Software, a gene expression analysis software package with powerful data viewing capabilities, is included. Data from up to 10 96-well RQ plates can be simultaneously analyzed. In an RQ study, you can:
• Select the endogenous control and the calibrator sample.
• Select the control type when applicable.
• Set baseline and threshold values and RQ Min⁄Max
Confidence Levels.
• Omit individual wells or sample replicates.

The user is able to view post-run amplification in three different formats, while gene expression plots show expression levels of target samples relative to the calibrator.

For Research Use Only. Not for use in diagnostics procedures.

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

Novex™ Tricine SDS Sample Buffer (2X) (Invitrogen™)

Novex Tricine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using tricine gels. Novex Tricine SDS Sample Buffer is specifically formulated for optimal electrophoresis of small proteins and peptides. The sample buffer is formulated with Coomassie Blue G and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie Blue G gives a sharp dye front with Tricine SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2 minutes for optimal results.

Recommended buffers: Use tricine running buffers with tricine sample buffers to obtain the benefits of this gel system.

See all buffers and reagents for SDS-PAGE ›

20X Bolt™ MOPS SDS Running Buffer (Invitrogen™)

Bolt MOPS SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris Plus gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris Plus gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 (Invitrogen™)

NuPAGE Tris-Acetate SDS Buffer Kit is designed for separation of medium to large size proteins on Tris-Acetate gels. This kit includes the following buffers:
• NuPAGE Tris-Acetate SDS Running Buffer (20X, Cat. No. LA0041)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

See all available buffers and reagents available for SDS-PAGE

7300 System SDS RQ Study Software (Applied Biosystems™)

The 7300 System SDS RQ Study Software is a gene expression analysis software package with powerful data viewing capabilities. Data from up to 10 96-well RQ plates can be simultaneously analyzed. In an RQ study, you can:
• Select the endogenous control and the calibrator sample.
• Select the control type when applicable.
• Set baseline and threshold values and RQ Min⁄Max
Confidence Levels.
• Omit individual wells or sample replicates.

The user is able to view post-run amplification in three different formats, while gene expression plots show expression levels of target samples relative to the calibrator.

For Research Use Only. Not for use in diagnostics procedures.

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

Sodium Dodecyl Sulfate (SDS), Lauryl (Thermo Scientific™)

Thermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE).

Features of Sodium Dodecyl Sulfate (Lauryl):

• Popular anionic detergent for a variety of protein methods
• Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE)
• Common component of cell lysis buffers

This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. The formulation is a mixture of several different alkyl sulfate chain lengths (C10 to C18).

Properties of SDS (values for pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate:

• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless to slightly yellow.
• Chain length: C12 >60%; C14 = 20 to 35%; C16 <10%; C10 and C18 <1% each

Related Products
Sodium Dodecyl Sulfate (SDS), C12
Sodium Dodecyl Sulfate (SDS), 20% Solution

NuPAGE™ MES SDS Running Buffer (20X) (Invitrogen™)

NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE