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In 1976, when treating dimethyl sulfoxide (DMSO) with trifluoroacetic anhydride (TFAA) below -50°C in dichloromethane, D.Swern and co-workers discovered that trifluoroacetoxydimethylsulfonium trifluoroacetate was formed, which reacted rapidly with primary and secondary amines.

In 1894, German chemist Emil Knoevenagel reported that diethyl malonate and formaldehyde condensed in the presence of diethylamine to form a bis-adduct. He later discovered that the same type of bis-adduct was produced when formaldehyde and other aldehydes were condensed with ethyl benzoylacetate or...

(±)-Propranolol hydrochloride (C 16 H 21 NO 2 · HCl), a nonselective beta-blocker, is representative of a class of drugs that target the beta receptor. It is prescribed medication for treatment of hypertension, severe headaches, and cardiac related dysrhythmia and ischemia.

In 1903, German chemist Bernhard Tollens observed that the reaction between acetophenone and formaldehyde in the presence of ammonium chloride led to the formation of a tertiary amine. In 1917, German chemist Carl Mannich also prepared a tertiary amine from antipyrine using the same conditions, and...

Introduction Hydrazides Alkoxyamines (aminooxy compounds) Reductive amination, Sulfhydryl-to-Carbohydrate Crosslinkers Hydrazide Biotinylation Reagents Carbohydrate Oxidant Aniline (GlycoLink coupling catalyst) Crosslinker Selection Tool Crosslinkers at a Glance, Aldehydes (RCHO) and ketones (RCOR')...

Biotinylation is widely used to enable isolation, separation, concentration, and further downstream processing and analysis of biomolecules. Proper biotinylation of the target molecule is essential to ensure high binding efficiency to the Invitrogen Streptavidin-Coupled Dynabeads.

Heterocyclic compounds, or heterocycles, are cyclic compounds that have atoms of at least two different elements as members of the ring structure.Organic heterocycles contain one or more carbon atoms and, based on their electronic structure, are classified as saturated, unsaturated, and aromatic.

We are dedicated to your success.Get back on track. View our expert recommendations for commonly encountered problem scenarios related to your protein assays and quantitation experiments. View the relevant questions below:, Browse our FAQ database for more information ›, Beginning your experiment?

Introduction to covalent immobilization Immobilization chemistries Amine-reactive supports Sulfhydryl-reactive supports Carbonyl-reactive immobilization methods Carbonyl-reactive supports Active hydrogen immobilization methods, Covalent Immobilization Supports Crosslinker Selection Guide Crosslinker...

Common ELISA formats., Introduction to enzyme probes Common enzyme probe reporters and chromogenic substrates Enzyme reporters for probes Types of substrates Enzyme labeling, Chemiluminescent Western Blot Detection Enzyme Substrates for ELISA Luciferase Assays, Enzymes are used to detect targets of...

Overview Summary of nucleic acid labeling methods Enzymatic methods for nucleic acid labeling Chemical methods for nucleic acid labeling Recommended reading, Pierce Biotin 3’ End DNA Labeling Kit LightShift Chemiluminescent EMSA Kit Terminal Deoxynucleotidyl Transferase, recombinant Pierce RNA 3’...

Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs., View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.

Douglas A. Hayworth, Ph.D.; Greg T. Hermanson, B.S., Protein biology research as we know it would not be possible without the variety of chromatography resins, beads, membranes, plastic surfaces and other solid or porous materials to immobilize proteins and affinity ligands.

Pesticide residues testing has to ensure compliance, with regard to maximum residues levels (MRLs) or tolerance levels of pesticides in foods. Analytical laboratories are expected to detect, quantitate and identify hundreds of different pesticides with diverse physicochemical properties in hundreds...

Qdot 525 probe is an excellent green-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~525 nm, Qdot 525 probe can be excited using a 488 nm laser line or standard FITC filter.