Product FAQ

For how long is Sodium Pyruvate (100 mM) stable at 2-8 degrees C after opening the bottle?

Answer

When stored properly at 2-8 degrees C, the product is stable for 12 months from date of manufacture.

Answer Id: E17411

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Product FAQ

What is the purpose of sodium pyruvate in media?

Answer

Sodium pyruvate serves as an additional energy source for cells in culture. It is often added to low-glucose formulations (1.0 g/L glucose) and is sometimes added to higher-glucose formulations as well. Cells can become “hooked” on sodium pyruvate however, and if it is withdrawn suddenly from the media, they may experience a short lag in growth.

Answer Id: E11882

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Product FAQ

Can I store Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) at -20 degrees C?

Answer

Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) has a shelf life of 18 months from date of manufacture when stored at 2-8 degrees C. In general, we do not recommend storing this product at -20 degrees C due to the potential issue of proteins precipitating from the solution upon thawing.

Answer Id: E17417

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Product FAQ

What is the advantage of using Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) Supplement in cultures?

Answer

ITS-A Supplement is designed as a supplement for RPMI- 1640 and Earle's Minimal Essential Medium (EMEM), and will enhance the growth of various adherent cell types at FBS concentrations < 4%.

Answer Id: E13277

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Product FAQ

How is your sodium pyruvate supplied?

Answer

Sodium pyruvate (Cat. No. 11360070) is supplied as a liquid supplement for cell culture media at 100 mM (100X) and a final concentration of 11,004 mg/L.

Answer Id: E11883

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Media Formulation

Neurobasal™-A Medium (1X) liquid

Catalog #
    0050128DJ(Discontinued)

Product FAQ

I am using one of your lentiviral vectors and am getting a low lentiviral titer. Can you offer some troubleshooting tips?

Answer

Possible causes include:

- low transfection efficiency; Use a high-quality plasmid prep, 293FT cells under passage 16, ensure removal of Geneticin during transfection, ensure correct DNA:lipid ratio, and that cells are plated at the correct confluency
- transfected cells are not cultured in medium containing sodium pyruvate; this reagent provides an extra energy source for cells
- viral supernatant harvested too early; viral supernatants can generally be collected 48-72 hrs post-transfection
- viral supernatant too dilute; concentrate virus using CsCl purification
- viral supernatant frozen and thawed multiple times; 3 times should be the maximum freeze/thaw
- gene of interest is large; viral titers decrease as size of insert increases, inserts larger than 5.6 kb are not recommended
- rearrangement in the LTR region of the epxression construct plasmid DNA; use Stb3 cells for transformatin of the lentiviral construct
- poor choice of titering cell line; use HT1080 cells or similar cell line
- Polybrene reagent is not included during transduction; transduce lentiviral construct into cells in the presence of Polybrene reagent
- Lipofectamine reagent handled incorrectly; ensure proper storage and mix gently before use
- Use fluorescence micrscopy to check titer with HiPerform FastTiter lentivirus

Answer Id: E9338

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Product FAQ

Does Leibovitz L-15 medium contain sodium bicarbonate?

Answer

No, Leibovitz L15 medium (liquid or powder) does not contain sodium bicarbonate (the formulation for each catalog number is published online). Developed for the support of cell growth in non CO2-equilibrated environments, L-15 formulations were developed without a sodium bicarbonate buffer system. L-15 medium is buffered by phosphates and free-base amino acids (L-arginine primarily, but also L-histidine and L-cysteine). Galactose and sodium pyruvate are used to replace glucose in order to reduce the production of acidic metabolites.

Answer Id: E13259

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Product FAQ

What precautions should I take with my 293FT cells to ensure high quality lentivirus production?

Answer

• Use low passage 293FT cells. Do not use 293FT cells beyond passage 20. Freeze down many aliquots and grow for 2–4 passages prior to transfection.
• Passage cells in complete D-MEM containing G418 (500 µg/mL). Supplement the media with "non-essential" amino acids and sodium pyruvate (0.1 mM MEM Non-Essential amino acids and 1 mM MEM Sodium Pyruvate). Use Gibco FBS (Cat. No. 16000-044).
• Plate cells at a density of 5 x 10e6 per 100 mm dish. Cell density is very important. Make sure that the cells are growing well before re-plating prior to the day of transfection. Avoid overgrowth of 293FT cells when passaging.
• When plating for transfection the next day, do not add G418 to the media.

Answer Id: E6402

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Manual / Product Insert

LanthaScreen c-Fos HeLa Cell-based Assay Protocol

Version: 14 May 2008
Catalog #
  • K1579(Discontinued)

Manual / Product Insert

GeneBLAzer® CMV-bla CHO-K1 Postive Control Cell Line Protocol

Version: Rev B 12/10

Manual / Product Insert

GeneBLAzer PPAR delta UAS-bla HEK 293T Cell Based Assay Protocol

Version: 8 November 2010
Catalog #

Manual / Product Insert

LanthaScreen™ STAT5 TF-1 T694/T699 Validation Packet

Version: version 1.1
Catalog #