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Application Note: Analysis of Trace Antimony in Water: Complying with Chinese Standard Methods for Atomic Absorption Spectroscopy Product Literature

What is the difference between the Quant-iT PicoGreen DNA, Quant-iT DNA, and Qubit DNA assays? Product FAQ

Answer

The Qubit Fluorometer contains highly optimized algorithms that calculate the concentration of the sample using either the Qubit assays or the Quant-iT DNA assays. The Quant-iT PicoGreen DNA assay may be adapted to the Qubit Fluorometer using the MyQubit firmware. The performance of all of these assays is similar.

The Quant-iT PicoGreen DNA assay is the most established assay and the most general-purpose (http://tools.thermofisher.com/content/sfs/manuals/PicoGreen-dsDNA-protocol.pdf). It requires the dilution of the standard DNA and buffer but can be adapted for use with either cuvettes, microplates, or the NanoDrop 3300.

The Quant-iT DNA assays provide a ready-to-use buffer and pre-diluted standard DNA for analyzing a large number of samples (>20 samples) using a 96-well microplate with no further adaptation.

The Qubit assays (https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-assays/myqubit.html) are intended for low throughput (<20 samples), and are only used on the Qubit Fluorometer.

Answer Id:: E8129

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High-pressure fluorescence correlation spectroscopy. Citations & References

  • Authors: Müller JD, Gratton E
  • Journal: Biophys J
  • PubMed ID: 14507734
Catalog #
  • F1300
  • R6479(Discontinued)

Probing ligand protein binding equilibria with fluorescence fluctuation spectroscopy. Citations & References

  • Authors: Chen Y, Müller JD, Tetin SY, Tyner JD, Gratton E
  • Journal: Biophys J
  • PubMed ID: 10920037
Catalog #
  • F1300
  • C183(Discontinued)

What are some common methods for analyzing protein-protein interactions? Product FAQ

Answer

Here are some common methods for analyzing protein-protein interactions:

- Co-immunoprecipitation (Co-IP)
- Pulldown assays
- Yeast two-hybrid screening
- Crosslinking
- Label transfer
- Immunofluorescence/fluorescence resonance energy transfer (FRET)
- Far-western analysis
- Surface plasmon resonance: relates binding information to small changes in refractive indices of laser light reflected from gold surfaces to which a bait protein has been attached. Changes are proportional to the extent of binding. Special labels and sample purification are not necessary, and analysis occurs in real time.
- NMR (nuclear magnetic resonance): Method that can provide insights into the dynamic interaction of proteins in solution.
- Mass Spectrometry: Used in concert with affinity-based methods (such as co-IPs) to isolate binding partners and complexes and to identify the component proteins using standard mass spectral methods (e.g., MALDI-TOF and mass searching of bioinformatics databases). Heavy (deuterated) crosslinkers can be used in conjunction with mass spectroscopy to elucidate details about protein-protein interactions.
- X-ray crystallography: crystallization of the interacting complex allows definition of the interaction structure.

Answer Id:: E15599

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The photon counting histogram in fluorescence fluctuation spectroscopy with non-ideal photodetectors. Citations & References

  • Authors: Hillesheim LN, Müller JD
  • Journal: Biophys J
  • PubMed ID: 12944307
Catalog #

Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Citations & References

  • Authors: Imbeaud S, Graudens E, Boulanger V, Barlet X, Zaborski P, Eveno E, Mueller O, Schroeder A, Auffray C
  • Journal: Nucleic Acids Res
  • PubMed ID: 15800207

Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Citations & References

  • Authors: Imbeaud S; Graudens E; Boulanger V; Barlet X; Zaborski P; Eveno E; Mueller O; Schroeder A; Auffray C
  • Journal: Nucleic Acids Research

GenotypePhenotype correlations in multiple sclerosis: HLA genes influence disease severity inferred by (1)HMR spectroscopy and MRI measures Citations & References

  • Authors: Okuda, DT; Srinivasan, R; Oksenberg, JR; Goodin, DS; Baranzini, SE; Beheshtian, A; Waubant, E; Zamvil, SS; Leppert, D; Qualley, P; Lincoln, R; Gomez, R; Caillier, S; George, M; Wang, J; Nelson, SJ; Cree, BAC; Hauser, SL; Pelletier, D
  • Journal: BRAIN
Catalog #

Determination of nitrite in waters by microplate fluorescence spectroscopy and HPLC with fluorescence detection. Citations & References

  • Authors: Büldt A, Karst U
  • Journal: Anal Chem
  • PubMed ID: 10450150
Catalog # M20490(Discontinued)

How can I prepare my exosome sample (isolated using the Total Exosome Isolation reagent) for analysis by mass spectroscopy? Product FAQ

Answer

The Total Exosome Isolation reagent allows recovery of the entire exosome population from the sample, in contrast to ultracentrifugation protocols, which recover significantly less material. Thus, the pellet is larger. The purity of exosomes is very similar for cell media samples (reagent vs ultra); for more challenging samples such as serum (which are more viscous and have much higher protein content, etc.) the reagent recovers exosomes at a lower purity (few microvesicles and large protein complexes co-precipitate) compared to ultracentrifugation. Having said this, you can obtain enough material for all standard types of downstream analysis, such as qRT-PCR or Western blot with either method.

Answer Id:: E7397

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Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application. Citations & References

  • Authors: Petersen NO, Höddelius PL, Wiseman PW, Seger O, Magnusson KE
  • Journal: Biophys J
  • PubMed ID: 8241393
Catalog # M8997

Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used. Citations & References

  • Authors: Gennerich A, Schild D
  • Journal: Biophys J
  • PubMed ID: 11106632
Catalog #

Continuous measurement of mitochondrial pH gradients in isolated hepatocytes by difference ratio spectroscopy. Citations & References

  • Authors: Thomas PJ, Gaspers LD, Pharr C, Thomas JA
  • Journal: Arch Biochem Biophys
  • PubMed ID: 1898020
Catalog #

Absolute Quantification of RNA Molecules Using Fluorescence Correlation Spectroscopy with Certified Reference Materials. Citations & References

  • Authors: Sasaki A, Yamamoto J, Kinjo M, Noda N
  • Journal: Anal Chem
  • PubMed ID: 30109932
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