Documents & Support

Near-Infrared (NIR) spectroscopy is a spectroscopic method that uses the near-infrared region of the electromagnetic spectrum. It is based on overtones and combinations of bond vibrations in molecules. In NIR spectroscopy, the unknown substance is illuminated with a broad-spectrum (many wavelengths or frequencies) of near infrared light, which can be absorbed, transmitted, reflected, or scattered by the sample of interest. Light is absorbed in varying amounts by the sample at particular frequencies corresponding to the combinations and overtones of vibrational frequencies of some bonds of the molecules in the sample. The NIR instrument analyzes the spectra and provides rapid, multi-component analysis. Although NIR spectroscopy is not as chemically specific as Raman or FTIR spectroscopy, it can be very useful in probing bulk material with little or no sample preparation.

Visit the NIR Technology page (https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/spectroscopy-elemental-isotope-analysis-learning-center/molecular-spectroscopy-information/nir-technology.html) for more information.

Was this answer helpful?

In Raman spectroscopy, an unknown sample of material is illuminated with monochromatic (single wavelength or single frequency) laser light, which can be absorbed, transmitted, reflected, or scattered by the sample. Light scattered from the sample is due to either elastic collisions of the light with the sample's molecules (Rayleigh scatter) or inelastic collisions (Raman scatter). Whereas Rayleigh scattered light has the same frequency (wavelength) of the incident laser light, Raman scattered light returns from the sample at different frequencies corresponding to the vibrational frequencies of the bonds of the molecules in the sample.

If you wish to learn more about Raman spectroscopy, visit our online Raman Spectroscopy Academy (https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/spectroscopy-elemental-isotope-analysis-learning-center/molecular-spectroscopy-information/raman-technology.html), where you will find basic Raman tutorials, advanced Raman webinars on sample applications, and a helpful instrument guide.
If you wish to learn more about Raman spectroscopy, visit our online Raman Spectroscopy Academy, where you will find basic Raman tutorials, advanced Raman webinars on sample applications, and a helpful instrument guide.

Was this answer helpful?

Please download and install the most current version of the Qubit Firmware from our website: https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources.html

For Qubit Fluorometers 1.0, 2.0 and 3.0 go to this web link: https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources/previous-qubit-models.html

Was this answer helpful?

NIR is a spectroscopic method that uses the near-infrared region of the electromagnetic spectrum. NIR spectroscopy is based on overtones and combinations of bond vibrations in molecules. NIR is utilized because it is a proven technology that delivers clear results when it comes to asbestos inspection, testing, and screening. You can learn more about the technology by visiting our NIR Technology website page (https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/spectroscopy-elemental-isotope-analysis-learning-center/molecular-spectroscopy-information/nir-technology.html).

Was this answer helpful?

Fourier Transform NIR (FTNIR) systems have advantages in higher resolution, better wavelength accuracy, higher signal energy, and are more stable and repeatable. FTNIR systems do not require software standardization for method transfer between instruments. Also FTNIR systems are not affected by stray light which causes sampling challenges for dispersive NIR systems.

Was this answer helpful?

The TruNarc Handheld Narcotics Analyzer uses lab-proven Raman spectroscopy. Raman is a vibrational spectroscopy technique where a single wavelength laser is focused on a sample. The laser excites the bonds of a molecule, which generates measurable scattered light to identify the material in question. Raman is a highly effective method for reliable identification of an unknown substance based on its underlying chemistry.

Raman spectroscopy has been extensively deployed for use in homeland security applications, largely due to the high chemical specificity, with each chemical substance having a unique spectral fingerprint.

Was this answer helpful?

Partial Least Squares (PLS) is a statistical approach to quantitative analysis. PLS models correlate spectral variation with component concentration variation to create models for component predictions.

Was this answer helpful?

Most handheld NIR spectroscopy instruments, including the microPHAZIR AS Asbestos Analyzer (https://www.thermofisher.com/order/catalog/product/MICROPHAZIRAS), can be handled by non-technical operators with limited training. Point-and-shoot operation provides results in seconds. Workers screening material can easily be trained and do not need to be experts in NIR spectroscopy to make decisions based on the device's results.

Was this answer helpful?

Although Raman spectroscopy is typically considered a non-destructive technique, conditions such as exposure time, laser power, and the nature of the sample may lead to sample degradation. The energy transmitted by the laser depends on the duration of exposure and the wavelength. It may change the physical state and may destroy the sample.

Was this answer helpful?

No, the Qubit Flex Fluorometer allows you to select which wells will be used for the experiment. The standards and sample will only be read in the selected wells. If the option to select wells is not available on your Qubit Flex, please update to the current firmware version (download firmware from Technical Resources for Qubit Fluorometers). This feature was introduced in version 1.5.0.

Was this answer helpful?

Please to go https://kb.unitylabservices.com/?cid=12825&c=13142&cpc=iCia805H4XUv1Ub1aS0kPQGY1OLg7MkTjK for support on our molecular spectroscopy products.

Was this answer helpful?

Traditional laboratory material verification workflows require moving materials into a sampling room, opening and sampling them, documenting samples for tracking purposes, sending samples to the lab, and moving materials into quarantine while awaiting lab results. With handheld NIR spectroscopy, pharmaceutical and dietary supplement manufacturers can verify the identity of incoming materials in the warehouse in minutes and avoid the time and costs of lab testing. Supplied materials such as API, bulking agents, lubricants, and solvents must be qualified at the point of entry into the factory to ensure finished product integrity. Portable NIR analyzers such as microPHAZIR RX Analyzer take NIR spectroscopy out of the lab and into the plant. Handheld NIR instruments allow non-expert users to obtain reliable material identity verification in the warehouse.

Was this answer helpful?

In Raman spectroscopy, an unknown sample of material is illuminated with monochromatic (single wavelength or single frequency) laser light, which can be absorbed, transmitted, reflected, or scattered by the sample. Light scattered from the sample is due to either elastic collisions of the light with the sample molecules (Rayleigh scatter) or inelastic collisions (Raman scatter). Whereas Rayleigh scattered light has the same frequency (wavelength) of the incident laser light, Raman scattered light returns from the sample at different frequencies corresponding to the vibrational frequencies of the bonds of the molecules in the sample.

If you wish to learn more about Raman spectroscopy, visit our online Raman Spectroscopy Academy (https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/spectroscopy-elemental-isotope-analysis-learning-center/molecular-spectroscopy-information/raman-technology.html), where you will find basic Raman tutorials, advanced Raman webinars on sample applications, and a helpful instrument guide.

The TruScan RM Handheld Raman Analyzer is built with a state-of-the-art optical platform paired with a field-proven embedded chemometrics engine. The patented, multivariate residual analysis offers the most effective chemometric solution for material identification, with two spectral pre-processing options (1st and 2nd derivative), that is easy to operate in challenging environments and sampling conditions.

In addition to Raman technology, the TruScan RM Handheld Raman Analyzer has TruTools, an optional embedded chemometrics software package (https://assets.thermofisher.com/TFS-Assets/CAD/Specification-Sheets/TSRM-TruTools-SpecSheet-2016-FINAL.pdf), with which users can build advanced, customized qualitative and quantitative methods for complex material analysis problems. TruTools models allow discrimination of multiple components, discrimination of raw materials with minimal spectral differences (such as ethanol and methylated spirits), and discrimination of low API dosage tablets from placebos. With TruTools deployed on a TruScan RM Handheld Raman Analyzer, non-expert operators can run advanced chemometric analyses anywhere in the plant.

Was this answer helpful?