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Real-time PCR (also known as quantitative PCR or qPCR) is the simultaneous amplification and detection/quantification of nucleic acids using the polymerase chain reaction (PCR). qPCR is a versatile technique anda powerful tool to support your research applications .

These Quant-iT products provide the maximum amount of dye in packaging that is amenable to highly automated quantitation environments. These are the best choice for quantitating more than 2,000 samples at atime.

The Quant-iT Kits, 1,000 assay size, provide the ultimate in convenience for DNA, RNA or protein quantitation in microplates. These are the best choice for quantitating 20–2,000 samples at a time. See our line of automation-ready Thermo Scientific microplate readers For quantitation of more than...

PCR (polymerase chain reaction) is an invaluable tool for molecular biology research. It is used in laboratories around the world in a wide array of applications such as cloning, gene expression analysis, genotyping, sequencing, and mutagenesis.

The USB product line for the life sciences is now integrated into either our Applied Biosystems or ThermoScientific brand portfolios, enhancing our wide range of products and kits for popular molecular biology applications.

Applied Biosystems genetic analysis systems are a trusted standard for Sanger sequencing and fragment analysis by capillary electrophoresis—proven through decades of results, including the first sequencing of the human genome and the discovery of genes implicated in diseases like cystic fibrosis.

Together, Ambion and Applied Biosystems (AB) provide researchers with a complete family of products that takes experiments from sample preparation through target detection and analysis. For limited samples and small sample sizes, RNA can be extracted from numerous small tissue samples with one of...

It’s true. We tested Applied Biosystems TaqMan Gene Expression Assays with TaqMan Fast Advanced Master Mix and PowerUp SYBR Green Master Mix on the Bio-Rad CFX96 and CFX384 Real-Time PCR Detection systems. Check out the results below.

Thermo Fisher Scientific has developed a manufacturing process using Single Use System (SUS)-based technology with extensive quality control testing to drastically reduce the risk of DNA contaminates in the PCR reagents for commercial supply.

The direct PCR approach delivers convenience for DNA amplification by allowing PCR directly from samples without prior DNA purification. Significant savings in time and cost can be achieved in applications such as plant and animal genotyping.

Fluorescent labeling of biomolecules enables researchers to detect specific components of complex biological assemblies with exquisite sensitivity in applications ranging from imaging and flow cytometry, to western blots.

Laser capture microdissection (LCM) is an extraordinarily gentle method ideal for isolating single cells or small numbers of cells for your research. We offer research-use reagents and kits for the complete LCM workflow—from identifying and selecting cells to isolating and purifying RNA and DNA.

The process for mRNA research and discovery requires flexible products and technologies to meet many of your diverse needs. To effectively facilitate mRNA discovery, Thermo Fisher Scientific offers a variety of products that span the entire portfolio from target gene identification and synthesis,...

Solid tumor research often includes analysis of the tumor and the use of techniques such as immunohistochemistry, DNA/RNA isolation, qRT-PCR, and next-generation sequencing and the application of these techniques to a variety of sample types.

We offer an extensive line of SYBR dye products to meet your needs for both real-time PCR and nucleic acid staining applications. Real-time polymerase chain reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time).