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Can you isolate exosomes from embroyonic stem cell (ESC) culture medium? What type of media and culture conditions do you recommend? Product FAQ

Answer

While we have not tested ESC, for those media that we have tested, the number of secreted exosomes and isolation protocols are very similar. When growing cells, use chemically defined media or exosome-depleted FBS. Exosomes can be collected at 24 hours. 1-5 mL of media is a good starting point to isolate exosomes. This should be enough for several real-time PCR or Western blotting experiments. Sequencing and some other downstream analyses may require more starting material, and for this, we would recommend 30 mL cell culture medium to start.

Answer Id: E7415

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Cell Culture Media - MarrowMAX Product Literature

Cell Culture Media and Reagent Product Literature

Can I use StemPro HSC Expansion Medium (Prototype) without cytokines or growth factors? Product FAQ

Answer

This media system is formulated without cytokines or growth factors, but cytokines and/or growth factors should be added immediately prior to use. For hematopoietic stem cell culture, we recommend adding the following cytokines at the final concentration indicated: SCF (100 ng/mL), FLT-3L (100 ng/mL), TPO (100 ng/mL), IL-3 (50 ng/mL), and IL-6 (20 ng/mL).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E16774

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How should I prepare my stem cells when I use Lipofectamine Stem Transfection Reagent? Product FAQ

Answer

Proliferating stem cells in culture need room to expand. We recommended plating cells so they will be 30-60% confluent at the time of transfection. For additional details, please refer to the Lipofectamine Stem Transfection Reagent protocol pertinent to your cell type and media on the product page (https://www.thermofisher.com/order/catalog/product/STEM00008?ICID=search-product), under Documents, Product Literature.

Answer Id: E15208

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Embryonic Stem Cell Culture Product Literature

Exosome CD63 (protocol for western blot) Manual / Product Insert

  • Pub. No.: 4696aed8b3bddf96d89883e506299510d5010fd5
  • Version: Rev 1.0 (2 June 2013)
Catalog # 10606D

Exosome CD63 (protocol for flow cytometry) Manual / Product Insert

  • Pub. No.: 2ec9d76e785774d7b12b3ca8925cd91264b50e7a
  • Version: Rev 1.0 (2 June 2013)
Catalog # 10606D

Total Exosome Isolation (from cell culture media) Manual / Product Insert

  • Pub. No.: ab902263560e96e43150ca68f668ab3d507dc4ce
  • Version: 1.0
Catalog # 4478359

BenchStable Cell Culture Media - Green Fact Sheet Product Literature

What aspects of cell culture media can be customized? Product FAQ

Answer

The Gibco Media Configurator allows you to add, remove, and adjust the concentration of components. In addition, you can select from a range of packaging, QC tests, and either cGMP or non-cGMP manufacturing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11845

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Is there a fast way to reorder Gibco cell culture media? Product FAQ

Answer

Yes. If you are reordering the same formulation, in the same quantity and packaging, simply use the Quick Order link found at the top of every page of thermofisher.com, or visit your Order History page and click on the Reorder box.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11858

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With Lipofectamine Stem Transfection Reagent, how can I improve the transfection efficiency in my stem cells? Product FAQ

Answer

To obtain the highest percent of stem cells transfected with Lipofectamine Stem Transfection Reagent, we highly recommend that you follow cell culture conditions that promote formation of a monolayer versus clumps of cells. If the density of your stem cells is greater than 50% or they are being grown as colonies in other media, more Lipofectamine Stem Transfection Reagent may improve transfection efficiency. For additional details, please refer to the Lipofectamine Stem Transfection Reagent protocol pertinent to your cell type and media on the product page (https://www.thermofisher.com/order/catalog/product/STEM00008?ICID=search-product), under Documents, Product Literature.

Answer Id: E15216

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How much serum should I add to my media? Product FAQ

Answer

The serum concentration will vary with the cell line and basal medium used. Please go here to see our recommended sera supplementations for tested cell lines (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/advanced-d-mem-and-mem/recommended-sera-supplementations.html).

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Answer Id: E11862

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Article: Next Generation Cell Culture Media and Feeds System Product Literature

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