Citations & References

Stem cell media culture of melanoma results in the induction of a nonrepresentative neural expression profile.

  • Authors: Anaka M, Freyer C, Gedye C, Caballero O, Davis ID, Behren A, Cebon J
  • Journal: Stem Cells 2012; (30):2 336-343
  • PubMed ID: 22102544

Product FAQ

What protocol is recommended for delivery of Cas9 protein:guide RNA complex to my PSCs cultured in StemFlex Medium?

Answer

We recommend use of the Neon Electroporation device for electroporation of PSCs with Cas9 protein:guide RNA complex following the protocol guidance in the following demonstrated protocol (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/PG1584-PJT1313-COL31227-Demonstrated-Protocol-Performing-CRISPR-Cas9-FHR.pdf); see the section entitled “Knockout by electroporation of RNP using the Neon Transfection System.” We have seen that the Neon electroporation protocols 7 and 14 provide optimal indel formation while maintaining cell survival. However, the electroporation conditions may need to be optimized for your pluripotent cell line.

Answer Id: E14762

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Product FAQ

I use TesR Medium as a PSC culture medium. Is PSC Definitive Endoderm Induction Medium compatible with this medium?

Answer

Optimal results are obtained by using Essential 8 Medium as a pluripotent stem cell medium, however mTesR1 or TeSR-E8 can be used as substitute, if desired.

Answer Id: E9385

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Citations & References

Effects of Cell Cycle Regulators on the Cell Cycle Synchronization of Porcine induced Pluripotent Stem Cells.

  • Authors: Kwon DJ, Hwang IS, Kwak TU, Yang H, Park MR, Ock SA, Oh KB, Woo JS, Im GS, Hwang S,
  • Journal: Dev Reprod (2017) 21:47-54
  • PubMed ID: 28484743
Catalog #

Citations & References

CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.

  • Authors: Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schäfer BW
  • Journal: PLoS One 2011; (6):5 e19506-e19506
  • PubMed ID: 21602936

Citations & References

Characterization and transplantation of induced megakaryocytes from hematopoietic stem cells for rapid platelet recovery by a two-step serum-free procedure

  • Authors: Chen, TW; Hwang, SM; Chu, IM; Hsu, SC; Hsieh, TB; Yao, CL
  • Journal: EXPERIMENTAL HEMATOLOGY 2009 11:1330-1339

Citations & References

Enhanced MDR1 Expression and Chemoresistance of Cancer Stem Cells Derived from Glioblastoma

  • Authors: Nakai, E; Park, K; Yawata, T; Chihara, T; Kumazawa, A; Nakabayashi, H; Shimizu, K
  • Journal: CANCER INVESTIGATION 2009 9:901-908
Catalog #
  • 4318739(Discontinued)
  • 4327058(Discontinued)
  • 4327059(Discontinued)

Product Literature

Pluripotent stem cell guidebook—key products and services for PSC research

Citations & References

Isolation and characterization of side population stem cells in articular synovial tissue.

  • Authors: Teramura T, Fukuda K, Kurashimo S, Hosoi Y, Miki Y, Asada S, Hamanishi C
  • PubMed ID: 18549504

Citations & References

Isolation, characterization, and differentiation of human multipotent dermal stem cells.

  • Authors: Li L, Fukunaga-Kalabis M, Herlyn M
  • Journal: Methods Mol Biol 2013; (989): 235-246
  • PubMed ID: 23483399

Citations & References

Isolation and characterization of side population stem cells in articular synovial tissue

  • Authors: Teramura, T; Fukuda, K; Kurashimo, S; Hosoi, Y; Miki, Y; Asada, S; Hamanishi, C

Product Literature

Serum-free supplement for neural stem and progenitor cells

Product Literature

Superior NSC expansion with serum-free medium

Product Literature

Brochure: Gibco Essential 8 Media