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TPO (Thrombopoietin), Recombinant Human Protein (Gibco™)

Recombinant Human Thrombopoietin (TPO) is a bioactive protein intended for use in cell culture applications. TPO is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, which is the product of the myeloproliferative leukemia virus oncogene. Recombinant Human Thrombopoeitin (TPO) is one of the many GIBCO® recombinant bioactive proteins available from Life Technologies. This is the 10 microgram size.

High purity—minimal interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—GIBCO® proteins bioassayed with GIBCO® media

High purity recombinant bioactive protein
Recombinant Human Thrombopoietin (TPO) is one of more than 250 GIBCO® recombinant proteins available from Life Technologies. To help ensure GIBCO® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to help ensure a biologically active protein.

High biological activity
In-house activity testing of Recombinant Human Thrombopoeitin (TPO) includes cell proliferation, cytotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, and protease assays. Recombinant Human Thrombopoietin (TPO) specific activity is determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Proven compatibility
GIBCO® proteins are bioassayed with GIBCO® media. Since 1962, GIBCO® has been a consistent provider of high quality media, reagents, and sera for reliable cell culture.

We also offer a selection of growth factors for use in cell therapy applications through our GIBCO® Cell Therapy Systems (CTS™) product line.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related links
See our selection of recombinant GIBCO® growth factors.
Learn more about the GIBCO® Cell Therapy Systems (CTS™) product line.

Thrombopoietin Human ELISA Kit (Invitrogen™)

Thrombopoietin Human ELISA Kit for ELISA

TPO Recombinant Mouse Protein (Gibco™)

Recombinant TPO is a bioactive protein intended for use in cell culture applications. TPO is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene.

Thrombopoietin Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Thrombopoietin Simplex ProcartaPlex kit measures thrombopoietin protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit for more information, including a comprehensive list of individual protein targets.


Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Thrombopoietin Mouse ELISA Kit (Invitrogen™)

Thrombopoietin (TPO/THPO) Mouse ELISA Kit for ELISA

Thrombopoietin Monoclonal Antibody (1B11) (Invitrogen™)

Thrombopoietin Monoclonal Antibody for Western Blot, Flow

Thrombopoietin Polyclonal Antibody (Invitrogen™)

Thrombopoietin Polyclonal Antibody for Western Blot

Thrombopoietin Polyclonal Antibody (Invitrogen™)

Thrombopoietin Polyclonal Antibody for Western Blot

Thrombospondin 1 Polyclonal Antibody (Invitrogen™)

Thrombospondin 1 Polyclonal Antibody for Western Blot, IF, ICC, IP