Our Gibco growth factors and Invitrogen recombinant proteins are produced in a range of expression systems including mammalian, E. coli, insect, and baculovirus to meet different cell culture application needs. Offering proven compatibility with Gibco cell culture media, these growth factors and recombinant proteins are designed to deliver superior results with less protein and with minimized interference from proteins or other contaminants.
TNF Recombinant Human Protein (Gibco™)
• High purity—no interference from other proteins or contaminants
• High biological activity—more results with less protein
• Proven compatibility—Gibco® proteins bioassayed with Gibco® media
High Purity Recombinant Bioactive Protein
Recombinant Human Tumor Necrosis Factor-α is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.
High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Tumor Necrosis Factor-α (TNF) specific activity is determined by the dose dependent cytotoxic effect on L929 cells in the presence of actinomycin D.
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.
See our selection of recombinant Gibco® growth factors.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
TNF alpha Human Uncoated ELISA Kit with Plates (Invitrogen™)
Human TNF alpha (TNFa) Recombinant Protein (Invitrogen™)
TNF Recombinant Rat Protein (Gibco™)
TNF alpha Mouse ProQuantum Immunoassay Kit (Invitrogen™)
TNF alpha Rat Uncoated ELISA Kit with Plates (Invitrogen™)
TNF alpha Monoclonal Antibody (MAb11), eBioscience™ (Invitrogen™)
TNF alpha Human Uncoated ELISA Kit (Invitrogen™)
Human TNF beta (TNFb) Recombinant Protein (Invitrogen™)
TNF alpha Porcine ELISA Kit (Invitrogen™)
TaqMan™ Array Human TNF Superfamily Pathway (Applied Biosystems™)
• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan® probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan®Assay Quality, New Format
TaqMan® Array Plates contain the same high quality TaqMan® Gene Expression Assays (PCR primers and TaqMan® probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan®Assays supplied in the traditional liquid format and assays dried into a TaqMan® Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.
Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan®Assay in each well: enough for one 20 µl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan®Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.
Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs
Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan® Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan®Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.
Simple to Use
Each TaqMan® Array Plate well contains a single TaqMan®Assay for one 20 µl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.
TaqMan® Array Plates have been optimized for use with TaqMan® Gene Expression Master Mix, but are also compatible with TaqMan® Universal PCR Master Mix. For studies using cultured cells, TaqMan® Array Plates are also compatible (and recommended) with the TaqMan® Gene Expression Cells-to-Ct™ Kit. TaqMan® Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems® 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 µl reaction volumes.