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CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™ (Invitrogen™)

CD120a (TNF Receptor I) Monoclonal Antibody for Neu, FN

TNF alpha Monoclonal Antibody (E10011) (Invitrogen™)

TNF alpha Monoclonal Antibody for Western Blot, ELISA

TNF alpha Human Matched Antibody Pair (Invitrogen™)

TNF alpha Human Matched Antibody Pair for ELISA

TNF alpha Recombinant Rabbit Monoclonal Antibody (17H1L4) (Invitrogen™)

TNF alpha Recombinant Monoclonal Antibody for Western Blot

TNF alpha Rat ELISA Kit (Invitrogen™)

TNF alpha Rat ELISA Kit for ELISA

TNF alpha Mouse Uncoated ELISA Kit (Invitrogen™)

TNF alpha Mouse Uncoated ELISA Kit for ELISA

Rat TNF alpha Recombinant Protein (Invitrogen™)

Rat TNF alpha Recombinant Protein for Western Blot, IP, ELISA, Ctrl

TNF alpha Monoclonal Antibody (MP6-XT3), Functional Grade, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Neu, FN

TNF alpha Monoclonal Antibody (MAb11), Alexa Fluor 488, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF Superfamily 15-Plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human TNF Superfamily 15-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 15 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
4-1BBL; APRIL; BAFF; CD30L; EDA-1; FasL; GITRL; CD40L; NGF beta; TNF alpha; TNF beta; LIGHT; RANKL; TRAIL; TWEAK

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] 4-1BBL: 168.95-692000 pg/mL; APRIL:72.53-297100 pg/mL; BAFF: 2.64-10800 pg/mL; CD30L: 185.1-758300 pg/mL; EDA-1: 28.25-28925 pg/mL; FasL: 2.2-9000 pg/mL; GITRL: 3.31-13575 pg/mL; CD40L: 5.15-21100 pg/mL; NGF beta: 8.47-34700 pg/mL; TNF alpha: 6.86-28100 pg/mL; TNF beta: 2.47-10125 pg/mL; LIGHT: 1.27-5200 pg/mL; RANKL: 7.71-31600 pg/mL; TRAIL: 1.99-8150 pg/mL; TWEAK: 37.6-154000 pg/mL

Assay Sensitivity
See product documentation

TNF alpha Monoclonal Antibody (MP6-XT22), PE-eFluor 610, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF beta Polyclonal Antibody (Invitrogen™)

TNF beta Polyclonal Antibody for Western Blot, IHC (P)

TNF (TNFa) Recombinant Human Protein

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Tumor necrosis factor alpha (TNF-α) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein is expressed from a DNA sequence encoding the soluble form of human TNF (NP_000585.2) (Val 77-Leu 233) retaining the initial Met at the N-terminus.

N-terminal Sequence Analysis: Met.

Activity: measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is typically 25-200 pg/mL, corresponding to a specific activity of 5 x 106 to 4 x 107 units/ mg.

Formulation: lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-α is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is primarily produced as a type I I transmembrane protein arranged in stable homotrimers, and a soluble trimeric form of TNF-α is released via proteolytic cleavage by the metalloprotease TACE/ ADAM17. Two receptors mediating the TNF-α function have been identified, the ubiquitous TNF RI from which most signaling are derived, and the hematopoietic cell-restricted TNF RII. Upon the TNF-α trimers binding, TNF receptors also form trimers, undergo a conformational change, and enable the adaptor protein TRADD to bind to the death domain, and thus initiates the signal pathways such as the NF-kB, Jak/STAT, and the MAPK pathways, as well as the death signaling. As a major mediator of apoptosis, inflammation and immunity, TNF-α has been implicated in the pathogenesis of a variety of human diseases including autoimmune diseases, insulin resistance, and cancer.

You may also be interested in the following TNF-α products:
ELISA Kits
Primary Antibodies
Pathway Analysis Kits

TNF alpha Monoclonal Antibody (TN3-19.12), eFluor 660, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

CD120a (TNF Receptor I) Monoclonal Antibody (H398), eBioscience™ (Invitrogen™)

CD120a (TNF Receptor I) Monoclonal Antibody for Flow, FN