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TaqMan™ Array, Human TNF Superfamily Pathway, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human TNF Superfamily Pathway 96-well Plate contains 92 assays to TNF Superfamily pathway associated genes and 4 assays to candidate endogenous control genes.

TNF alpha Porcine ELISA Kit (Invitrogen™)

TNF alpha Porcine ELISA Kit for ELISA

TNF alpha (Total) Human ELISA Kit (Invitrogen™)

TNF alpha (Total) Human ELISA Kit for ELISA

Feline TNF Alpha Recombinant Protein (Invitrogen™)

Feline TNF Alpha Recombinant Protein for Western Blot, IP, ELISA

TNF Recombinant Human Protein, Liquid Form (Gibco™)

Recombinant Human Tumor Necrosis Factor-α (TNF) is a bioactive protein intended for use in cell culture applications. TNF causes cytolysis and cytostasis of many tumor cell lines. TNF has a wide spectrum of activities, including chemotaxis of neutrophils, alteration of the endothelium, inhibition of anticoagulatory mechanisms, and promotion of angiogenesis.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Tumor Necrosis Factor-α is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Tumor Necrosis Factor-α (TNF) specific activity is determined by the dose dependent cytotoxic effect on L929 cells in the presence of actinomycin D.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF alpha Polyclonal Antibody (Invitrogen™)

TNF alpha Polyclonal Antibody for Western Blot, ELISA

TNF-alpha Rat ProcartaPlex™ Simplex Kit (Invitrogen™)

The Rat TNF-alpha Simplex ProcartaPlex kit measures TNF-alpha protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

CD120b (TNF Receptor II) Monoclonal Antibody (TR75-54), eBioscience™ (Invitrogen™)

CD120b (TNF Receptor II) Monoclonal Antibody for Western Blot, Flow, IP, ELISA, FN

TNF alpha Monoclonal Antibody (MAb11), PE-Cyanine7, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF alpha (Total) Monkey ELISA Kit (Invitrogen™)

TNF alpha (Total) Monkey ELISA Kit for ELISA

TNF alpha Human Instant ELISA™ Kit (Invitrogen™)

TNF alpha Human Instant ELISA™ Kit for ELISA

Soluble TNF-RI Recombinant Human Protein (Gibco™)

Recombinant Human Soluble TNF-Receptor Type I (sTNF-RI) is a bioactive protein intended for use in cell culture applications. sTNF-RI is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-κB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High purity recombinant bioactive protein
Recombinant Human Soluble TNF-Receptor Type I is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High biological activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Soluble TNF-Receptor Type I (sTNF-RI) specific activity is determined by measuring the inhibition of TNF-α mediated cytotoxicity in murine L929 cells in the presence of 0.25 ng/ml TNF-α.

Proven compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

TNF alpha Monoclonal Antibody (MAb11), PerCP-Cyanine5.5, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow