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TNF Recombinant Human Protein (Gibco™)

Recombinant Human Tumor Necrosis Factor-α (TNF) is a bioactive protein intended for use in cell culture applications. TNF causes cytolysis and cytostasis of many tumor cell lines. TNF has a wide spectrum of activities, including chemotaxis of neutrophils, alteration of the endothelium, inhibition of anticoagulatory mechanisms, and promotion of angiogenesis.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Tumor Necrosis Factor-α is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Tumor Necrosis Factor-α (TNF) specific activity is determined by the dose dependent cytotoxic effect on L929 cells in the presence of actinomycin D.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

TNF alpha Human ELISA Kit (Invitrogen™)

TNF alpha Human ELISA Kit for ELISA

TNF alpha Human ELISA Kit, Ultrasensitive (Invitrogen™)

TNF alpha Human ELISA Kit, Ultrasensitive for ELISA

TNF alpha Recombinant Polyclonal Antibody (3HCLC) (Invitrogen™)

TNF alpha Recombinant Polyclonal Antibody for Western Blot

Human TNF alpha Recombinant Protein (Invitrogen™)

Human TNF alpha Recombinant Protein for Western Blot, IP, ELISA, Ctrl

TNF alpha Mouse ELISA Kit, High Sensitivity (Invitrogen™)

TNF alpha Mouse ELISA Kit, High Sensitivity for ELISA

TNF alpha Monoclonal Antibody (1F3F3D4), eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for ELISA

TNF alpha Antibody Cocktail, Biotin, eBioscience™ (Invitrogen™)

TNF alpha Cocktail Antibody for ELISA

TNF alpha Polyclonal Antibody, Biotin, eBioscience™ (Invitrogen™)

TNF alpha Polyclonal Antibody for ELISA

TNF beta Human Instant ELISA™ Kit (Invitrogen™)

TNF beta Human Instant ELISA™ Kit for ELISA

TNF alpha Monoclonal Antibody (MAb11), eFluor 450, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF alpha Monoclonal Antibody (2TNF-H33), Biotin (Invitrogen™)

TNF alpha Monoclonal Antibody for Western Blot, ELISA

TNF alpha Monoclonal Antibody (MP6-XT22), FITC, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

TNF-alpha Porcine ProcartaPlex™ Simplex Kit (Invitrogen™)

The Porcine TNF-alpha Simplex ProcartaPlex kit measures TNF-alpha protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Pig

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

TNF beta Monoclonal Antibody (359-81-11), APC, eBioscience™ (Invitrogen™)

TNF beta Monoclonal Antibody for Flow

TNF alpha Mouse Uncoated ELISA Kit with Plates (Invitrogen™)

TNF alpha Mouse Uncoated ELISA Kit with Plates for ELISA

TNF Recombinant Mouse Protein (Gibco™)

Recombinant Mouse Tumor Necrosis Factor-α (TNF) is a bioactive protein intended for use in cell culture applications. TNF causes cytolysis and cytostasis of many tumor cell lines. TNF has a wide spectrum of activities, including chemotaxis of neutrophils, alteration of the endothelium, inhibition of anticoagulatory mechanisms, and promotion of angiogenesis.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Mouse Tumor Necrosis Factor-α is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Mouse Tumor Necrosis Factor-α (TNF) specific activity is determined by the dose dependent cytotoxicity of L929 cells.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Human TNF beta (TNFb) Recombinant Protein (Invitrogen™)

Human TNF beta Recombinant Protein for Western Blot, IP, ELISA, Ctrl

TNF alpha Mouse ProcartaPlex™ Simplex Kit, High Sensitivity (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

TNF-beta Monkey ProcartaPlex™ Simplex Kit (Invitrogen™)

The Non-Human Primate TNF-beta Simplex ProcartaPlex kit measures TNF-beta protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Monkey

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

TNF alpha Polyclonal Antibody, Biotin (Invitrogen™)

TNF alpha Polyclonal Antibody for Western Blot, ELISA

TNF alpha Mouse ELISA Kit (Invitrogen™)

TNF alpha Mouse ELISA Kit for ELISA

TNF alpha Monoclonal Antibody (TN3-19.12), PE, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

Mouse TNF alpha ELISA Standard Recombinant Protein, eBioscience™ (Invitrogen™)

Mouse TNF alpha ELISA Standard Recombinant Protein for ELISA, Ctrl

TNF beta Human ELISA Kit (Invitrogen™)

TNF beta Human ELISA Kit for ELISA

CD120a (TNF Receptor I) Monoclonal Antibody (TBP-1), eBioscience™ (Invitrogen™)

CD120a (TNF Receptor I) Monoclonal Antibody for ELISA

TNF alpha Polyclonal Antibody (Invitrogen™)

TNF alpha Polyclonal Antibody for Western Blot

TNF alpha Monoclonal Antibody (MP6-XT22), PerCP-eFluor 710, eBioscience™ (Invitrogen™)

TNF alpha Monoclonal Antibody for Flow

CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™ (Invitrogen™)

CD120a (TNF Receptor I) Monoclonal Antibody for Neu, FN

TNF alpha Monoclonal Antibody (2TNF-H34A) (Invitrogen™)

TNF alpha Monoclonal Antibody for Western Blot, ELISA