Sort By

TNFR (Soluble-80 kDa) Human ELISA Kit (Invitrogen™)

TNFR (Soluble-80 kDa) Human ELISA Kit for ELISA

Soluble TNF-RII Recombinant Human Protein (Gibco™)

Recombinant Soluble TNF-RII is a bioactive protein intended for use in cell culture applications. sTNF-RII is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.

TNFR2 Monoclonal Antibody (MR2-1), PE (Invitrogen™)

TNFR2 Monoclonal Antibody for Flow

TNF-RII Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human TNF-RII Simplex ProcartaPlex kit measures TNF-RII protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

TNFRII (Soluble) Human ELISA Kit (Invitrogen™)

TNFRII (Soluble) Human ELISA Kit for ELISA

TNFR2 (soluble) Monoclonal Antibody (897C 2G9 1H3) (Invitrogen™)

TNFR2 (soluble) Monoclonal Antibody for ELISA

TNFR2 Polyclonal Antibody (Invitrogen™)

TNFR2 Polyclonal Antibody for IHC (P)

TNFR2 Polyclonal Antibody (Invitrogen™)

TNFR2 Polyclonal Antibody for IHC (P)

TRAIL-R2 (DR5) Monoclonal Antibody (DR-5-01-1), FITC (Invitrogen™)

TRAIL-R2 (DR5) Monoclonal Antibody for Flow

TRAIL-R2 (DR5) Monoclonal Antibody (DR5-01-1), FITC (Invitrogen™)

TRAIL-R2 (DR5) Monoclonal Antibody for Flow

TNFR2 Monoclonal Antibody (22221.311) (Invitrogen™)

TNFR2 Monoclonal Antibody for Neu

TNFR2 (soluble) Polyclonal Antibody (Invitrogen™)

TNFR2 (soluble) Polyclonal Antibody for Western Blot

TRAIL-R2 (DR5) Polyclonal Antibody (Invitrogen™)

TRAIL-R2 (DR5) Polyclonal Antibody for Western Blot, IF, ICC, IHC (P), Flow

TRAIL-R2 (DR5) Polyclonal Antibody (Invitrogen™)

TRAIL-R2 (DR5) Polyclonal Antibody for Western Blot, IHC (P)

TNFR2 Polyclonal Antibody (Invitrogen™)

TNFR2 Polyclonal Antibody for Western Blot, IP