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TNFSF13B Recombinant Human Protein, hIgG1-Fc Tag

Tumor Necrosis Factor Super Family 13 subunit beta (TNFSF13B), recombinant human/mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the soluble form of human TNFSF13B (Q9Y275-1) (Ala 134-Leu 285) fused to the Fc region of human IgG1 at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized recombinant human TNFSF13B at 1 µg/ml (100 µl/well) can bind human TNFRSF17
. The EC50 of human TNFRSF17 is 0.07 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

TNFSF13B Monoclonal Antibody (OTI7F12), TrueMAB™ (OriGene)

TNFSF13B Monoclonal Antibody for Western blot

BAFF Antibody (9H23L24), ABfinity™ Rabbit Monoclonal (Invitrogen™)

BAFF Monoclonal Antibody for Western Blot

BAFF Human Instant ELISA™ Kit (Invitrogen™)

BAFF Human Instant ELISA™ Kit for ELISA

BAFF Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human BAFF Simplex ProcartaPlex kit measures BAFF protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

BAFF Recombinant Human Protein (Gibco™)

Recombinant BAFF is a bioactive protein intended for use in cell culture applications. Recombinant BAFF is intended for use in cell culture applications. BAFF is important in B-cell maturation and survival.

BAFF Antibody (12HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

BAFF Oligoclonal Antibody for Western Blot, IHC (P)

BAFF Mouse ProcartaPlex™ Simplex Kit (Invitrogen™)

The Mouse BAFF Simplex ProcartaPlex kit measures BAFF protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

BAFF Polyclonal Antibody (Invitrogen™)

BAFF Polyclonal Antibody for Western Blot, ICC

BAFF Monoclonal Antibody (Buffy-1), FITC (Invitrogen™)

BAFF Monoclonal Antibody for Western Blot, IF, ICC, Flow

BAFF (soluble) Monoclonal Antibody (Buffy 2) (Invitrogen™)

BAFF (soluble) Monoclonal Antibody for Western Blot, IHC (P), Flow

BAFF (soluble) Monoclonal Antibody (Buffy-1) (Invitrogen™)

BAFF (soluble) Monoclonal Antibody for Western Blot, IF, ICC, Flow

BAFF Monoclonal Antibody (2B) (Invitrogen™)

BAFF Monoclonal Antibody for ELISA

BAFF Polyclonal Antibody (Invitrogen™)

BAFF Polyclonal Antibody for Western Blot, IF, ICC

BAFF Polyclonal Antibody (Invitrogen™)

BAFF Polyclonal Antibody for Western Blot