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What is the main purpose of TMT (Tandem Mass Tag) reagents? Product FAQ

Answer

TMT technology is used to perform multiplex quantitation of proteins extracted from cells and tissues. Rather than quantify single proteins (for example with an antibody), here, large numbers of proteins can be quantified in a single run.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E17287

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What is the main purpose of Tandem Mass Tag (TMT) reagents? Product FAQ

Answer

Tandem Mass Tag technology is used to individually label different protein samples so they can be combined into a single sample for LC-MS analysis. The major advantages of this workflow are higher sample throughput, less instrument analysis time, high precision of peptide quantitation among replicates, and fewer missing quantified proteins among different samples.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Answer Id: E18802

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What is the Pierce TMT11plex Yeast Digest Standard and what is its main purpose? Product FAQ

Answer

The Pierce TMT11plex Yeast Digest Standard is a lyophilized yeast peptide mixture of four congenic strains labeled with TMT11plex reagents. It serves as a standard for quality assessment of LC and MS data for samples labeled with Tandem Mass Tag (TMT) reagents.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E17281

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User Guide: Iodoacetyl Tandem Mass Tag (iodoTMT) Reagents Manual / Product Insert

  • Version: MAN0011795 Rev.B (08Feb2016)
Catalog #

What type of mass spectrometer can I use for Tandem Mass Tag (TMT) analysis? Product FAQ

Answer

We recommend using high-resolution Orbitrap Tribrid (e.g., Fusion, Lumos, Eclipse), Orbitrap Exploris (e.g., 240, 480), or Q Exactive (e.g,. Plus, HF, HFX) series of instruments.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Answer Id: E18803

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Which standard do you recommend using for Tandem Mass Tag (TMT) workflows? Product FAQ

Answer

We recommend using our Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for LC-MS method optimization and for assessing system suitability for TMT-labeled samples.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Answer Id: E18818

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Instructions: TMT Mass Tagging Reagents Manual / Product Insert

  • Version: MAN0015866 Rev. A.0 (27Sep2016)
Catalog #
  • 90309
  • 90308(Discontinued)

Flyer: Tandem Mass Tag call for applications Product Literature

What is the difference between Relative and Absolute Quantitation? Which type of quantitation is used for proteomic samples? Product FAQ

Answer

Relative quantitation compares the amount of an analyte in different samples. Absolute quantitation measures the actual amount of an analyte in a sample using a known standard at different concentrations. Label-free, SILAC, and TMT (Tandem Mass Tag) workflows are used for relative quantitation of protein samples. Heavy (i.e., AQUA) peptides are used as standard for absolute quantitation of target proteins.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Answer Id: E18801

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Flyer: 4th annual Thermo Scientific Tandem Mass Tag Research Award Product Literature

Flyer: 5th Annual Thermo Scientific Tandem Mass Tag Research Awards Product Literature

After running the Pierce TMT11plex Yeast Digest Standard, I observe low signal to noise ratio and poor quantitation accuracy for the Tandem Mass Tag (TMT) reporter ions. What do you recommend? Product FAQ

Answer

Here are some recommendations:

- Clean and recalibrate the mass spectrometry instrument using one of our Pierce Calibration Solutions (https://www.thermofisher.com/search/browse/category/us/en/600654).
- Verify settings for liquid chromatography (LC) acquisition methods.
- Fractionate TMT-labeled samples using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat. No. 84868) to reduce sample complexity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Answer Id: E18824

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Relative Quantitation of Protein Digests Using Tandem Mass Tags and Pulsed-Q Dissociation (PQD) Product Literature

User Guide: aminoxyTMT Mass Tag Labeling Reagents Manual / Product Insert

  • Version: MAN0011892 Rev.B (08Feb2016)
Catalog #

Application Note: Relative Quantitation of Protein Digests Using Tandem Mass Tags and Pulsed-Q Dissociation (PQD) Product Literature

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