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Why are my PCR tubes crushed/deformed after my PCR run? Product FAQ

Answer

The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:

Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.

Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).

Answer Id:: E7643

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What is the difference between the MicroAmp Optical Tubes (P/N801-0933) and the ABI PRISM Optical Tubes (P/N 4316567)? Is there any difference in the quality of the plastic used for the two different types? Product FAQ

Answer

There is no difference between these tubes.The MicroAmp Optical Tubes (P/N N801-0933) and the ABI PRISM Optical Tubes (P/N 4316567) are both made of a high-grade polypropylene. The tubes are frosted on the bottom and the caps provide for enhanced light transfer. Using non-optical tubes can give rise to significant attenuations in signal and therefore will lead to potential decreases in sensitivity in real-time assays.

Answer Id:: E2573

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I recently purchased a new bag of Qubit Assay Tubes (Cat. No. Q32856) and they look different than the tubes I received before. Are they okay to use? Product FAQ

Answer

We have changed the manufacturer of the Qubit Assay Tubes and the new tubes are slightly longer and more opaque than the original tubes. The new tubes have been tested in each of the Qubit instruments, with all of the Qubit assays, and using the fluorescent dyes, fluorescein and Alexa Fluor 647, to validate that there are no changes to the data quality when compared with the original Qubit tubes. The new tubes met or exceeded the current specifications for both the core and extended ranges for each of the Qubit assays, and were able to achieve the same limit of detection (LOD) as the original Qubit tubes for each of the Qubit assays and the fluorescent dyes tested.

Answer Id:: E15592

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I have other microfuge tubes available in the lab, including FACS tubes. Can I use the PrimeFlow RNA Assay with other microfuge or FACS tubes? Product FAQ

Answer

The use of other microfuge tubes or polystyrene FACS tubes during the hybridization steps of the PrimeFlow RNA protocol may result in significant cell loss and weaker signal. The microfuge tubes provided with the PrimeFlow RNA Assay have been validated to minimize cell loss and maximize signal. We highly recommend that you only use the provided microfuge tubes. However, cells may be fixed and permeabilized in bulk using polypropylene conical tubes (e.g. Fisher Scientific Cat. No. 14-959-70C). A modified protocol for the use of polystyrene, 96-well plates is also available in Appendix 7 of the PrimeFlow RNA Assay User Manual.

Answer Id:: E14480

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What is contained in Genexus Primer Pool Tubes (Cat. No. A40262)? Product FAQ

Answer

The Genexus Primer Pool Tubes kit contains 50 empty assemblies (2 tubes/assembly) of the custom tubes required to deliver primer oligos into the automated library preparation reaction. Each assembly is serialized for traceability and can be used with Ion AmpliSeq- and Ion AmpliSeq HD-based assays. Pipet an aliquot of panel primers from a custom panel or Ion AmpliSeq On-Demand Panel to run the assay on the Genexus Integrated Sequencer. The kit also contains a bag of 100 caps.

Note:
- Assays using Ion AmpliSeq chemistry have primer pools that are loaded in capped tubes in position 1 of the primer pool tube carriers. Empty uncapped tubes are loaded in position 2.
- Assays using Ion AmpliSeq HD chemistry have FWD and REV primer pools that are loaded in capped tubes in both positions of the primer pool tube carrier.

Answer Id:: E18186

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How do I load tube strips on the StepOne and StepOnePlus Real-Time PCR Systems? Product FAQ

Answer

Follow these instructions for proper loading of tubes, tube strips, and plates:

- Put the tubes or tube strips in the tray with the adapter on the 96-well support base.
- If using a plate, put the plate directly on the 96-well support base.
- Pipet your reactions into the tubes/tube strips or the wells on the plate.
- Seal the tube/tube strips with optical flat caps.
- If using a plate, seal the plate with either optical adhesive film or flat caps.

For optimal performance on the StepOnePlus instrument with partial loads:
- Load at least 16 tubes and arrange them in adjacent columns of 8 tubes, using rows A through H, or adjacent rows of 8 tubes, using columns 3 through 10.
For optimal performance on the StepOne instrument, load at least 4 tubes in the sample block.

Answer Id:: E7204

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What kind of tubes do I need to buy for the Invitrogen Qubit Fluorometer? Product FAQ

Answer

Use thin-wall, clear 0.5 mL PCR tubes such as Invitrogen Qubit Assay Tubes (http://www.thermofisher.com/order/catalog/product/Q32856) or Axygen PCR-05-C tubes (Fisher Scientific, Part Number 14-222-292). Other types of tubes can have autofluorescence or a frosted area for writing and may interfere with the assay.

Answer Id:: E10262

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Do you need to change the Neon electrolytic buffer tubes when changing cells or constructs? Product FAQ

Answer

The electrolytic buffer tubes are disposables and you should change the tubes when changing cells in order to avoid cross contamination. With regard to plasmid DNA, the answer depends on the sensitivity of your downstream assay. If slight plasmid cross-contamination does not affect your assay, you may not have to change the tubes. Please perform pilot experiments to determine whether it works for you. However, we still recommend changing the electrolytic buffer. In case you run out, the tubes can be purchased separately (Catalog number: MPT100).

Answer Id:: E5517

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Do you have any specific recommendation on the type of non-stick tubes to use for diluting the Poly-A RNA Control Stock? Product FAQ

Answer

The following tubes were used in-house during product development of this Poly-A RNA Control Kit, and we have not tested any other tubes at this time:
Non-stick RNase-free 0.5 mL microfuge tubes, Ambion, Cat. No. 12350
Non-stick RNase-free 1.5 mL microfuge tubes, Ambion, Cat. No. 12450

Answer Id:: E14385

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Is there a good stopping point in the workflow for the MagMAX for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus Blood RNA Tubes (Cat. No. 4451893)? Product FAQ

Answer

The user guide for our MagMAX for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus Blood RNA Tubes, states that you can stop after the RNA resuspension step by storing the resuspended crude RNA pellet on ice for up to 4 hours.
Please see page 18 of the following user guide: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/cms_084749.pdf.

Answer Id:: E18925

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Can I immerse Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid nitrogen (liquid phase) for storage? Product FAQ

Answer

Of the Nunc Biobanking and Cell Culture Cryogenic Tubes, we recommend the internally threaded design, incorporating a silicon gasket, for liquid nitrogen immersion. At ultra low temperatures, any sealing material becomes inflexible. A theoretical risk of leakage is present when any mechanical closure is used. To facilitate leakproof storage, mechanically closed containers must be hermetically sealed in a protective envelope.

The Nunc cryo products includes Nunc CryoFlex Tube Wrap, a heat sealable polyethylene tubing designed for cryogenic tube encasement. The use of cryogenic tubes for freezing in liquid nitrogen (liquid phase) storage without CryoFlex tubing poses a safety hazard.
WARNING: Do not use Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid phase of liquid nitrogen unless correctly sealed in Nunc CryoFlex Tube Wrap (Cat. No. 343958). Improper use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up, resulting in possible explosion or biohazard release. Use appropriate safety procedures as outlined in the Nalge Nunc International Cryopreservation Manual when handling and disposing of the vials.

Answer Id:: E17756

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